Innovative products for the molecular cloning workflow

The molecular cloning workflow requires multiple steps to obtain high-quality cloned DNA for your downstream applications. Help advance your molecular cloning workflow at every step and accelerate construction of recombinant clones.

Our wide range of cloning solutions address needs from routine to specialty applications, helping you find optimal reagents to achieve fast results with greater confidence. 


Molecular cloning workflow

Click on each step to explore the molecular cloning workflow and select products for your downstream applications that fit your research.

Find products for your cloning experiments by exploring 5 steps of the cloning workflow

Select from a variety of reagents and instruments to generate your fragment of interest from DNA, RNA, or synthetic fragments.

Up next: Step 2, Cloning ›

Discover reagents and techniques across the molecular cloning workflow, with solutions ranging from restriction enzymes to gene synthesis, designed for speed and simplicity to help you obtain high-quality cloned DNA for your next discovery.

Discover more about the protein expression workflow

Up next: Step 3, Transformation

The choice of competent cells for transformation depends upon the transformation methods, strain genotypes, plasmid characteristics, and desired applications. Explore a wide range of chemically competent and electrocompetent E. coli cells for low- and high-throughput cloning.

Up next: Step 4, Plasmid preparation ›

Once the bacterial cells are transformed with the vector and constructs selected, it is critical to recover plasmid DNA with maximum yield, purity, and integrity. Choose a method best suited to your workflow from column-based, magnetic bead-based, or automated solutions.

Up next: Step 5, Construct analysis ›

Choosing the right tools for your cloning analysis can significantly improve and accelerate results, enabling you to address downstream applications sooner. Select either the Nanodrop or Qubit benchtop instruments for your DNA sample quantification. Discover our innovative E-Gel Power Snap Electrophoresis systems, and explore our precast E-gel options to help accelerate your DNA analysis.

Select from a variety of reagents and instruments to generate your fragment of interest from DNA, RNA, or synthetic fragments.

Up next: Step 2, Cloning ›

Discover reagents and techniques across the molecular cloning workflow, with solutions ranging from restriction enzymes to gene synthesis, designed for speed and simplicity to help you obtain high-quality cloned DNA for your next discovery.

Discover more about the protein expression workflow

Up next: Step 3, Transformation

The choice of competent cells for transformation depends upon the transformation methods, strain genotypes, plasmid characteristics, and desired applications. Explore a wide range of chemically competent and electrocompetent E. coli cells for low- and high-throughput cloning.

Up next: Step 4, Plasmid preparation ›

Once the bacterial cells are transformed with the vector and constructs selected, it is critical to recover plasmid DNA with maximum yield, purity, and integrity. Choose a method best suited to your workflow from column-based, magnetic bead-based, or automated solutions.

Up next: Step 5, Construct analysis ›

Choosing the right tools for your cloning analysis can significantly improve and accelerate results, enabling you to address downstream applications sooner. Select either the Nanodrop or Qubit benchtop instruments for your DNA sample quantification. Discover our innovative E-Gel Power Snap Electrophoresis systems, and explore our precast E-gel options to help accelerate your DNA analysis.

Stylesheet for Classic Wide Template adjustments
Hero banner dark gradient overlay

開始進行基因選殖

我們提供了功能強大且多用途的Invitrogen選殖和表現載體、GeneArt基因合成與組裝工具,以及您實驗裡關鍵第一步驟所需要的分子生物學相關基礎產品。 不確定如何開始?了解更多資訊

基因選殖入門簡介          觀看我們的基因選殖技術指南

精選基因選殖專題目錄

僅需在室溫5分鐘反應的選殖流程,無須凝膠純化或PCR反應後修飾步驟以及 TOPO cloning的重組效率高達95%。

結合便利的Invitrogen線上訂購系統以及先進的GeneArt產品服務
採用Invitrogen Zero Blunt Cloning Kit達成超高效率的平末端選殖
透過Type IIs限制酶,對多個DNA插入片段進行非重組式選殖
以TOPO XL-2 Complete PCR Cloning Kit輕鬆進行長片段的基因選殖
Gateway基因重組選殖技術允許基因穿梭(gene shuttling),讓您幾乎可裝載至任何表現系統
TA選殖技術的基礎是利用胸腺嘧啶(thymine)和腺嘌呤(adenine)的互補性,以Invitrogen ExpressLink T4 DNA接合酶提高效能
根據應用需求、細胞株種類、包裝規格及轉化效率來選擇您的勝任細胞
通用型緩衝液系統搭載全新一代的限制酶和DNA修飾酶,簡化您的選殖流程
PCR酶適合於下列任一應用:高保真性、高特異性、長片段、GC含量高、基因分型,或者常規PCR

提供不同容量、包裝方式、修飾性和純度,滿足您的基因選殖需求

Thermo Scientific系列產品,包含FastDigest限制酶、CloneJET試劑盒、InsTAclone試劑盒、無須接合反應的選殖技術、鹼性磷酸酶等

選殖過程中可同時組裝最多15個片段,構築較長且無縫的殖株

在治療藥品的探索和開發階段,可全程對數據資料進行追踪和管理
協助您搜尋合成基因產物所需的工具、試劑盒或服務,包括客製化片段、基因組裝和基因突變的解決方案,以及從線上訂購經過優化的基因可輕鬆殖入您想要的載體

訂購指南

購買以下指定試劑,可享促銷價格 及 買二送一優惠,不可錯過! 只需使用折扣碼 RCLO13,優惠日期至 2020 年 3 月31 日。
了解更多 ›

NEW!

Catalog #NameSize2020 TWPRPromo Price
K1231CloneJET PCR Cloning Kit20 reactions6,6204,303
K1232CloneJET PCR Cloning Kit40 reactions12,8008,320
Catalog #NameSize2020 TWPRPromo Price
R0392IPTG, dioxane-free5 g8,9405,811
R0393IPTG, dioxane-free5 x 5 g27,20017,680
R0402X-Gal2 x 1 g17,20011,180
R0404X-Gal1 g10,9007,085
R0551Glycogen, RNA grade2 x 0.1 mL3,6102,347
R0561Glycogen, molecular biology grade2 x 0.25 mL3,1202,028
R0581Water, nuclease-free4 x 1.25 mL 850 553
R0582Water, nuclease-free30 mL1,5501,008
R0601DEPC-treated Water30 mL1,7801,157
R0603DEPC-treated Water5 x 1.0 mL 980 637
R0861DTT (dithiothreitol)5 g4,2302,750
R0862DTT (dithiothreitol)25 g17,20011,180
R0941X-Gal Solution, ready-to-use10 mL5,6903,699
R1021EDTA (0.5 M), pH 8.05 x 1.0 mL 960 624
R1171IPTG Solution, ready-to-use10 x 1.5 mL3,4802,262
B52TBE Buffer (Tris-borate-EDTA) (10X)1 L2,5601,664
R2801TopVision Agarose Tablets200 tablets (0.5g each)6,4104,167
R2802TopVision Agarose Tablets1,000 tablets (0.5g each)25,10016,315
R0491TopVision Agarose100 g6,7304,375
R0492TopVision Agarose500 g23,70015,405
Catalog #NameSize2020 TWPRPromo Price
B64FastDigest Buffer (10X)5 x 1 mL800520
B72FastDigest Green Buffer (10X)5 x 1 mL820533
FD0014FastDigest AluI100 reactions3,3202,158
FD0024FastDigest Alw21I100 reactions3,4402,236
FD0034FastDigest Alw26I100 reactions3,4302,230
FD0044FastDigest Alw44I200 reactions2,9401,911
FD0054FastDigest BamHI800 reactions2,6701,736
FD0055FastDigest BamHI2500 reactions5,4603,549
FD0064FastDigest BcnI200 reactions3,6602,379
FD0074FastDigest BglI200 reactions3,1602,054
FD0083FastDigest BglII100 reactions1,440936
FD0084FastDigest BglII200 reactions2,6701,736
FD0094FastDigest Bpu1102I50 reactions3,8302,490
FD0124FastDigest Bsp119I200 reactions2,7701,801
FD0134FastDigest Bsp120I200 reactions4,5902,984
FD0143FastDigest Bsu15I50 reactions1,6601,079
FD0144FastDigest Bsu15I100 reactions3,0401,976
FD0154FastDigest BsuRI400 reactions4,1402,691
FD0184FastDigest Cfr10I50 reactions3,6502,373
FD0194FastDigest Cfr13I100 reactions3,5902,334
FD0214FastDigest Csp6I100 reactions3,1402,041
FD0224FastDigest DraI200 reactions3,3302,165
FD0234FastDigest Eam1104I50 reactions3,5802,327
FD0244FastDigest Eam1105I100 reactions3,6202,353
FD0254FastDigest Ecl136II100 reactions2,8801,872
FD0264FastDigest EcoO109I200 reactions3,8402,496
FD0274FastDigest EcoRI800 reactions2,6501,723
FD0275FastDigest EcoRI2500 reactions5,4603,549
FD0293FastDigest Eco31I50 reactions1,7501,138
FD0294FastDigest Eco31I100 reactions3,3302,165
FD0303FastDigest Eco32I200 reactions1,7601,144
FD0304FastDigest Eco32I400 reactions3,1202,028
FD0314FastDigest Eco47I200 reactions3,3702,191
FD0324FastDigest Eco47III20 reactions4,4802,912
FD0334FastDigest Eco52I50 reactions3,7702,451
FD0344FastDigest Eco57I20 reactions3,3902,204
FD0364FastDigest Eco72I200 reactions3,6802,392
FD0374FastDigest Eco81I50 reactions3,3602,184
FD0384FastDigest Eco88I200 reactions3,4002,210
FD0394FastDigest Eco91I200 reactions3,2802,132
FD0404FastDigest Eco105I50 reactions3,0802,002
FD0414FastDigest Eco130I200 reactions2,8801,872
FD0424FastDigest Eco147I100 reactions3,1302,035
FD0434FastDigest ScaI100 reactions3,0802,002
FD0443FastDigest EheI20 reactions1,7601,144
FD0444FastDigest EheI50 reactions4,3402,821
FD0454FastDigest Esp3I20 reactions3,6902,399
FD0464FastDigest GsuI20 reactions5,4803,562
FD0474FastDigest Hin1I100 reactions3,6502,373
FD0484FastDigest Hin6I200 reactions3,4602,249
FD0494FastDigest HincII100 reactions3,1702,061
FD0504FastDigest HindIII800 reactions2,6601,729
FD0505FastDigest HindIII2500 reactions5,5103,582
FD0514FastDigest HpaII200 reactions3,0802,002
FD0524FastDigest KpnI300 reactions2,6601,729
FD0534FastDigest Kpn2I100 reactions3,1502,048
FD0544FastDigest MspI400 reactions2,8401,846
FD0554FastDigest MvaI200 reactions3,2102,087
FD0564FastDigest MluI100 reactions3,3002,145
FD0573FastDigest NcoI20 reactions850553
FD0574FastDigest NcoI100 reactions2,8601,859
FD0575FastDigest NcoI300 reactions6,8904,479
FD0583FastDigest NdeI100 reactions980637
FD0584FastDigest NdeI300 reactions2,6701,736
FD0585FastDigest NdeI1000 reactions20,800 13,520
FD0593FastDigest NotI20 reactions1,450943
FD0594FastDigest NotI50 reactions3,3802,197
FD0595FastDigest NotI150 reactions8,1605,304
FD0596FastDigest NotI250 reactions12,2007,930
FD0604FastDigest PaeI20 reactions2,6401,716
FD0614FastDigest PstI800 reactions2,9401,911
FD0615FastDigest PstI2500 reactions6,0403,926
FD0624FastDigest PvuI20 reactions3,1102,022
FD0634FastDigest PvuII400 reactions2,6701,736
FD0644FastDigest SalI200 reactions2,8801,872
FD0654FastDigest SduI50 reactions3,3302,165
FD0663FastDigest SmaI100 reactions1,6001,040
FD0664FastDigest SmaI200 reactions2,8901,879
FD0674FastDigest TaqI400 reactions3,3702,191
FD0684FastDigest XbaI300 reactions3,1102,022
FD0685FastDigest XbaI750 reactions6,6004,290
FD0694FastDigest XhoI400 reactions2,6501,723
FD0695FastDigest XhoI1200 reactions6,1503,998
FD0704FastDigest Bst1107I100 reactions3,6302,360
FD0714FastDigest Van91I100 reactions3,6402,366
FD0724FastDigest BclI300 reactions2,7901,814
FD0734FastDigest Mph1103I100 reactions3,5802,327
FD0744FastDigest CpoI20 reactions3,6102,347
FD0753FastDigest MunI20 reactions1,520988
FD0754FastDigest MunI50 reactions3,6202,353
FD0764FastDigest Psp5II50 reactions3,4702,256
FD0774FastDigest SspI100 reactions3,3602,184
FD0784FastDigest Bsp143I40 reactions3,1202,028
FD0804FastDigest HinfI400 reactions2,8901,879
FD0814FastDigest MboI50 reactions3,5602,314
FD0824FastDigest MboII50 reactions3,0401,976
FD0834FastDigest BspTI150 reactions2,8201,833
FD0854FastDigest Pfl23II50 reactions4,3702,841
FD0874FastDigest BseGI100 reactions3,7402,431
FD0884FastDigest BseNI100 reactions3,9602,574

For Research Use Only. Not for use in diagnostic procedures.