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Thermo Scientific™
DH10B Competent Cells
Thermo Scientific DH10B Competent Cells are high efficiency, chemically competent E. coli cells. The DH10B strain is suitable for cloningRead more
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Catalog number EC0113
Price (USD)/ 10 x 100 µL
158.65
Online exclusive
177.00
Save 18.35 (10%)
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Price (USD)/ 10 x 100 µL
158.65
Online exclusive
177.00
Save 18.35 (10%)
Add to cart
DH10B Competent Cells
Catalog numberEC0113
Price (USD)/ 10 x 100 µL
158.65
Online exclusive
177.00
Save 18.35 (10%)
-
Add to cart
Thermo Scientific DH10B Competent Cells are high efficiency, chemically competent E. coli cells. The DH10B strain is suitable for cloning DNA containing methylcytosine, 5-hydroxymethylcytosine, and methyladenine, allowing both prokaryotic and eukaryotic genomic DNA to be cloned efficiently. These cells are ideal for the construction of gene banks or for the generation of cDNA libraries using plasmid-derived vectors. The φ80dlacZΔM15 marker provides α-complementation of the β-galactosidase gene from pUC or similar vectors to allow blue/white colony screening on bacterial agar plates containing Bluo-Gal or X-Gal.
• High transformation efficiency: >1x109 cfu/μg pUC19 DNA • Suitable for routine and high-throughput cloning applications • Allows for blue/white colony screening • Convenient two reactions per vial packaging • S.O.C. Outgrowth medium included
Applications DH10B Competent Cells are suitable for variety of applications requiring high transformation efficiency: • Efficient DNA cloning both of prokaryotic and eukaryotic origin • Cloning of large plasmids and BACs • Ideal for generation of cDNA libraries using plasmid-derived vectors • Site directed mutagenesis
Note: Optimized for use with the CloneJET PCR Cloning Kit (Cat. Nos. K1231, K1232) and Phusion Site-Directed Mutagenesis Kit (Cat. No. F541), and available as combo versions (Cat. Nos. K123120, K123240, F542).
For Research Use Only. Not for use in diagnostic procedures.