Phusion High-Fidelity PCR Master Mix with HF or GC Buffer
| Catalog Number | Includes | No. of Reactions |
|---|---|---|
| F532S | GC Buffer | 100 Reactions |
| F532L | GC Buffer | 500 Reactions |
| F531L | HF Buffer | 500 Reactions |
| F531S | HF Buffer | 100 Reactions |
Phusion High-Fidelity PCR Master Mix is convenient 2X mix containing Phusion DNA Polymerase, nucleotides, and optimized reaction buffer including MgCl2. Two master mix formulations are available: with HF Buffer (F-531S and F-531L) and with GC Buffer (F-532S and F-532L). The error rate of Phusion DNA Polymerase in HF Buffer (4.4 x 10-7) is lower than that in GC Buffer (9.5 x 10-7). Therefore, the master mix with HF Buffer should be used as the default master mix for high-fidelity amplification. However, GC Buffer can improve the performance of Phusion DNA Polymerase on some difficult or long templates, such as GC-rich templates or those with complex secondary structures.
Highlights
- High fidelity (25X Taq)
- Fast PCR due to short extension times (15–30 s/kb)
- Robust performance, minimal optimization needed
Applications
- Amplification of difficult (GC-rich) templates
- High-fidelity PCR
- Cloning
- Template generation for sequencing
- Long-range PCR (up to 20 kb)
- Mutagenesis
- High throughput PCR
Using Phusion DNA polymerases
Annealing rules for Phusion DNA polymerases are different from many common DNA polymerases (such as Taq DNA polymerases).
• 100% DMSO (1 x 500 μL)
Store at –20°C.