19 96 well plates—some in white and some in black

Select 96-well cell culture plates by surface

Whether culturing individual cell lines or scaling up for high-throughput screening, there is a Thermo Scientific Nunc 96-well plate for your needs. Advances in surface modifications, well geometry and optical clarity mean a variety of 96-well cell culture plate tailored for your specific application, including optical bottom plates for cellular imaging.

To help ensure reliability, flexibility, and reproducibility across every stage of cell culture, plates are available with a variety of surfaces optimized for different needs in cell attachment and cell growth.

Nunc Edge 2.0 96-well plate 96-well optical bottom plates

Nunclon Supra product photo

NEW Nunclon Supra Surfaces for advanced cell culturing

We are proud to provide a new product line surface treatment that will support improved cell yield and morphology as well enabling you to do xeno-free or low-serum applications.

Order yours today:  Dishes   |  Flasks  |  Plates  |  96-well plates

See how our surfaces perform with 39 cell types and cell lines at thermofisher.com/supradata

Non-treated

Non-treated surface for suspension culture and general biochemical assays

A macro of a non-treated cultureware surface

Made from high-quality USP Class VI virgin polystyrene, solid Nunc 96-well plates (in white and black) are ideal for colorimetric, fluorescence-, and luminescence-based cell analysis as well as protein binding assays

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Clear, flat bottom 96-well non-treated plates

Cat. No.DescriptionSurfaceColorLidSterilePack sizeCase size 
269620Nunc 96-Well Flat BottomNon-treatedClearNo
NoSleeve of 560Order
260860Nunc 96-Well Flat BottomNon-treatedClearYesYesSleeve of 10160Order
266120Nunc 96-Well Flat BottomNon-treatedClearYesYesSleeve of 25100Order
243656Nunc 96-Well Flat BottomNon-treatedClearYesYesSleeve of 6108Order
269787Nunc 96-Well Flat BottomNon-treatedClearNoYesIndividually packed50Order
260895Nunc 96-Well Flat BottomNon-treatedClearYesNoSleeve of 10160Order
260844Nunc 96-Well Flat Bottom, BarcodedNon-treatedClearNoNoSleeve of 10180Order
260887Nunc 96-Well Flat Bottom, BarcodedNon-treatedClearYesYesSleeve of 10160Order
260836Nunc 96-Well Flat BottomNon-treatedClearNoNoSleeve of 10180Order
Sleeve of 10
Sleeve of 10

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Black, flat bottom, polystyrene 96-well non-treated plates

Cat. No.DescriptionSurfaceColorLidSterilePack SizeCase Size 
237108Nunc 96-Well Flat BottomNon-treatedBlackNoNoSleeve of 10180Order
237105Nunc 96-Well Flat BottomNon-treatedBlackYesYesIndividually packed50Order
237107Nunc 96-Well Flat BottomNon-treatedBlackYesYesSleeve of 10160Order

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White, flat bottom, polystyrene 96-well non-treated plates

Cat. No.DescriptionSurfaceColorLidSterilePack SizeCase Size 
236108Nunc 96-Well Flat BottomNon-treatedWhiteNoNoSleeve of 10180Order
236105Nunc 96-Well Flat BottomNon-treatedWhiteYesYesIndividually packed50Order
236107Nunc 96-Well Flat BottomNon-treatedWhiteYesYesSleeve of 10160Order

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Clear, round (U) bottom, polystyrene 96-well non-treated plates

Cat. No.DescriptionSurfaceColorLidSterilePack SizeCase Size 
268152Nunc 96-Well U BottomNon-treatedClearNoNoSleeve of 10180Order
268200Nunc 96-Well U BottomNon-treatedClearYesYesSleeve of 10160Order
262162Nunc 96-Well U BottomNon-treatedClearNoYesIndividually packed50Order
278606Nunc 96 Deep Well U BottomNon-treatedClearNoYesSleeve of 432Order
278605Nunc 96 Deep Well U BottomNon-treatedClearNoNoSleeve of 432Order
262146Nunc 96-Well U-BottomNon-treatedClearNoNoBulk pack of 135135Order

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Clear, conical (V) bottom, polystyrene 96-well non-treated plates

Cat. No.DescriptionSurfaceColorLidSterilePack SizeCase Size 
249570Nunc 96-Well V BottomNon-treatedClearNoNoSleeve of 10180Order
249662Nunc 96-Well V BottomNon-treatedClearNoYesIndividually packed50Order
249952Nunc 96-Well V BottomNon-treatedClearYesYesSleeve of 10160Order
249935Nunc 96-Well V BottomNon-treatedClearYesYesSleeve of 1080Order
249940Nunc 96-Well V BottomNon-treatedClearNoYesSleeve of 1080Order

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Nunclon Delta surface

Nunc 96-well cell culture treated plates for adherent cells

The Nunclon Delta surface is a fully synthetic energy-treated surface that makes the otherwise very hydrophobic polystyrene surface more hydrophilic, thus facilitating cell attachment and growth. Our Nunclon Delta products are subjected to rigorous quality control procedures to meet the standards of Thermo Fisher Scientific and to ensure lot-to-lot consistency.

Explore cell performance with Nunclon Delta surface

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Flat bottom, polystyrene 96-well cell-culture-treated plates

Cat. No.DescriptionSurfaceColorLidSterilePack sizeCase size 
167008Nunc 96-Well Flat BottomNunclon DeltaClearYesYesIndividually packed50Order
161093Nunc 96-Well Flat BottomNunclon DeltaClearYesYesSleeve of 10160Order
136101Nunc 96-Well Flat BottomNunclon DeltaWhiteYesYesIndividually packed50Order
136102Nunc 96-Well Flat BottomNunclon DeltaWhiteYesYesSleeve of 10160Order
137101Nunc 96-Well Flat BottomNunclon DeltaBlackYesYesIndividually packed50Order
137103Nunc 96-Well Flat BottomNunclon DeltaBlackYesYesSleeve of 10160Order
168055Nunc 96-Well Flat BottomNunclon DeltaClearNoYesIndividually packed50Order

Round (U) bottom, polystyrene 96-well cell-culture-treated plates

Cat. No.DescriptionSurfaceColorLidSterilePack sizeCase size 
163320Nunc 96-Well U BottomNunclon DeltaClearYesYesIndividually packed50Order
168136Nunc 96-Well U BottomNunclon DeltaClearYesYesSleeve of 10160Order
143761Nunc 96-Well U BottomNunclon DeltaClearNoYesIndividually packed50Order
Nunclon Sphera surface

Nunclon Sphera surface-treated plates for ultra-low binding surface

The Nunclon Sphera surface-treated plates offer a physiologically relevant 3D cell culture format for disease modeling and drug screening applications in cancer and stem cell research, designed to enable the production of reproducible spheroids that can be further differentiated into specific organoids.

Explore the Nunclon Sphera surface and request samples

Cat. No.DescriptionSurfaceColorLidSterilePack sizeCase size 
174925Nunclon Sphera 96-Well U BottomNunclon SpheraClearYesYesIndividually packed8Order
174929Nunclon Sphera 96-Well U BottomNunclon SpheraClearYesYesSleeve of 550Order
174927Nunclon Sphera 96-Well Flat BottomNunclon SpheraClearYesYesIndividually packed8Order
Nunclon Supra surface

Nunc 96-well Supra treated plates for adherent cells

Graphed cell yield data

Thermo Scientific Nunc 96-well plates with Supra surface treatment are designed for culturing finicky adherent cells (primary and MSCs). When results matter most, Supra surface is designed to deliver higher cell yield, reduced time-to-adherence, and better morphology.

Nunclon Supra surface is more hydrophilic than the Nunclon Delta surface, enhancing the growth of finicky cell types under no-serum (xeno-free culture) or low-serum conditions.

See how Supra performs on 39 cells/cell lines

Nunc UpCell surface

Nunclon UpCell surface-treated plates for trypsin free dissociation

The Nunc UpCell surface-treated plates are grafted with PIPAAm polymers that change conformation and hydrophilicity according to external temperature, dislodging cells from the growth surface in this process while keeping cellular membrane and surface receptors intact.

Explore the Nunclon UpCell surface

Nunc UpCell 96-well plate for culturing and harvesting adherent cells without trypsin

Cat. No.DescriptionSurfaceColorLidSterilePack sizeCase size 
174897Nunc UpCell 96 Flat Bottom UpCellClearYesYesIndividually packed8Order
ECM surface

Nunc ECM treated plates for finicky cells

Nunc poly-D-lysine (PDL)- and collagen I-coated plates are designed to enhance the adhesion and growth of primary cells and finicky cell lines in vitro. The PDL synthetic material and the collagen I protein of animal origin are aseptically coated in either the Nunc regular plates or the Nunc Optical Bottom plates with 10-3 sterility assurance level (SAL).

Explore PDL- and collagen I-coated surfaces

Cat. No.DescriptionSurfaceColorLidSterilePack sizeCase size 
152038Nunc 96-Well Flat BottomCollagen IClearYesYesSleeve of 520Order
152039Nunc 96-Well Flat BottomPoly-D-LysineClearYesYesSleeve of 520Order
152036Nunc 96-Well Optical Polystyrene Polymer BottomCollagen IBlackYesYesSleeve of 520Order
152040Nunc 96-Well Optical Polystyrene Polymer BottomCollagen IWhiteYesYesSleeve of 520Order
152037Nunc 96-Well Optical Polystyrene Polymer BottomPoly-D-LysineBlackYesYesSleeve of 520Order
152028Nunc 96-Well Optical Polystyrene Polymer BottomPoly-D-LysineWhiteYesYesSleeve of 520Order


Nunc Edge 2.0 96-well plate with moat

Thermo Scientific Nunc Edge 2.0 96-well cell culture plates contain a large evaporation moat that can be filled with sterile water or media to help eliminate edge effect.

This edge effect is caused when evaporation occurs in the perimeter wells of a microplate during prolonged incubation. It changes media pH, osmolality, and the concentration of the media constituents, affecting cell viability and function.

However, the built-in moat in the Nunc Edge 2.0 plate significantly reduces evaporation compared to the conventional 96-well plates after four-day incubation, improving well-to-well consistency.

A chart showing superior cell viability when using Edge 2.0 plates
To prevent evaporation, simply:
A 96-well plate being filled with desired cell culture

Utilize all 96 wells

A Nunc Edge 2.0 plate exterior moat in the process of being filled

Fill surrounding moat with 2 mL
sterile water or media in each section

A close-up image of water evaporating from the 2.0 moat but not from the wells themselves.

Watch as evaporation happens in the moat and not the outer wells

Order Nunc Edge 2.0 96-well plates
Cat. No.DescriptionSurfaceColorLidSterilePack sizeCase size 
167425

Nunc Edge 2.0 96-Well Plate Treated

Nunclon Delta

Clear

Yes

Yes

Individually packed

50

Order
167542

Nunc Edge 2.0 96-Well Plate Treated

Nunclon Delta

Clear

Yes

Yes

Sleeve of 10

160

Order
167574

Nunc Edge 2.0 96-Well Plate Treated

Nunclon Delta

Clear

No

Yes

Individually packed

50

Order
167554

Nunc Edge 2.0 96-Well Plate Treated

Nunclon Delta

Clear

No

Yes

Sleeve of 10

160

Order
267427

Nunc Edge 2.0 96-Well Plate Non-treated

Non-treated

Clear

Yes

Yes

Individually packed

50

Order
267544

Nunc Edge 2.0 96-Well Plate Non-treated

Non-treated

Clear

Yes

Yes

Sleeve of 10

160

Order
267576

Nunc Edge 2.0 96-Well Plate Non-treated

Non-treated

Clear

No

Yes

Individually packed

50

Order
267556

Nunc Edge 2.0 96-Well Plate Non-treated

Non-treated

Clear

No

Yes

Sleeve of 10

160

Order
267566

Nunc Edge 2.0 96-Well Plate Non-treated

Non-treated

Clear

No

No

Sleeve of 10

160

Order
267578

Nunc Edge 2.0 96-Well Plate Non-treated

Non-treated

Clear

Yes

No

Sleeve of 10

160

Order
Sleeve of 10
Sleeve of 10


96-well plate with optical bottom

Close up of neurons in green and purple

Optical bottom plates for cellular imaging

With superior imaging quality and minimal background noise and crosstalk between wells, Nunc optical bottom plates are optimized for fluorescence and luminescence imaging applications.

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Optical polystyrene polymer bottom, 96-well plates

Cat. No.DescriptionSurfaceColorLidSterilePack sizeCase size 
165305

Nunc 96-Well Optical Polystyrene Polymer Bottom

Nunclon Delta

Black

Yes

Yes

Sleeve of 10

30

Order
165306

Nunc 96-Well Optical Polystyrene Polymer Bottom

Nunclon Delta

White

Yes

Yes

Sleeve of 10

30

Order
152036

Nunc 96-Well Optical Polystyrene Polymer Bottom

Collagen I

Black

Yes

Yes

Sleeve of 5

20

Order
152040

Nunc 96-Well Optical Polystyrene Polymer Bottom

Collagen I

White

Yes

Yes

Sleeve of 5

20

Order
152037

Nunc 96-Well Optical Polystyrene Polymer Bottom

Poly-D-lysine

Black

Yes

Yes

Sleeve of 5

20

Order
152028

Nunc 96-Well Optical Polystyrene Polymer Bottom

Poly-D-lysine

White

Yes

Yes

Sleeve of 5

20

Order
265301

Nunc 96-Well Optical Polystyrene Polymer Bottom

Non-treated

Black

No

No

Sleeve of 10

30

Order
265302

Nunc 96-Well Optical Polystyrene Polymer Bottom

Non-treated

White

No

No

Sleeve of 10

30

Order

Optical coverglass bottom, 96-well plates

Cat. No.DescriptionSurfaceColorLidSterilePack sizeCase size 
164588

Nunc 96-Well Optical 1.5 Coverglass Bottom

Cell culture-treated

Black

Yes

Yes

Sleeve of 6

30

Order
164590

Nunc 96-Well Optical 1.5 Coverglass Bottom

Cell culture-treated

White

Yes

Yes

Sleeve of 6

30

Order
160376

Nunc 96-Well Optical 1.5 CC2 Coverglass Bottom

CC2 enhanced cell culture

Black

Yes

Yes

Sleeve of 6

30

Order
265300

Nunc 96-Well Optical 1.5 Coverglass Bottom

Non-treated

Black

No

No

Sleeve of 5

30

Order

Resources

For Research Use Only. Not for use in diagnostic procedures.