Summary of Molecular Probes secondary antibody conjugates—Table 7.1

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Table 7.1 Summary of Molecular Probes secondary antibody conjugates.

Antibody	Host	Table No.
Anti–mouse IgG	Goat	Table 7.1A
Anti–mouse IgM	Goat	Table 7.1J
Anti–rabbit IgG	Goat	Table 7.1B
Anti–rat IgG	Goat	Table 7.1C
Anti–rat IgM	Goat	Table 7.1J
Anti–human IgG	Goat	Table 7.1D
Anti–human IgM	Goat	Table 7.1J
Anti–chicken IgG	Goat	Table 7.1G
Anti–guinea pig IgG	Goat	Table 7.1H
Anti–hamster IgG	Goat	Table 7.1I
Anti–mouse IgG	Rabbit	Table 7.1A
Anti–rat IgG	Rabbit	Table 7.1C
Anti–human IgG	Rabbit	Table 7.1D
Anti–goat IgG	Rabbit	Table 7.1F
Anti–mouse IgG	Chicken	Table 7.1A
Anti–rabbit IgG	Chicken	Table 7.1B
Anti–rat IgG	Chicken	Table 7.1C
Anti–human IgG	Chicken	Table 7.1D
Anti–goat IgG	Chicken	Table 7.1F
Anti–mouse IgG	Donkey	Table 7.1A
Anti–rabbit IgG	Donkey	Table 7.1B
Anti–rat IgG	Donkey	Table 7.1C
Anti–sheep IgG	Donkey	Table 7.1E
Anti–goat IgG	Donkey	Table 7.1F

Table 7.1A Molecular Probes affinity-purified anti–mouse IgG antibody conjugates.

Cat. No.	Label	Emission Color	Abs *	Em *
Goat Anti–Mouse IgG
A11045	Alexa Fluor 350	Blue	346	442
M10991	Marina Blue	Blue	365	460
C962	Cascade Blue	Blue	400	420
A31553	Alexa Fluor 405	Blue	402	421
P10993	Pacific Blue	Blue	410	455
A11063	Alexa Fluor 430	Yellow-green	434	539
F2761	Fluorescein	Green	494	518
A11001	Alexa Fluor 488	Green	495	519
O6380	Oregon Green 488	Green	496	524
B2752	BODIPY FL	Green	505	513
O6383	Oregon Green 514	Green	511	530
A31555	Alexa Fluor 514	Yellow-green	518	540
A11002	Alexa Fluor 532	Yellow	531	554
A10521	Cy3	Orange	552	570
T2762	Tetramethylrhodamine	Orange	555	580
A11003	Alexa Fluor 546	Orange	556	573
A21422	Alexa Fluor 555	Orange	555	565
P852	R-phycoerythrin (R-PE)	Orange	496, 546, 565	578
A20980	Alexa Fluor 610–R-PE	Red	496, 546, 565	628
A20990	Alexa Fluor 647–R-PE	Far-red	496, 546, 565	668
A20983	Alexa Fluor 680–R-PE	Far-red	496, 546, 565	702
R6393	Rhodamine Red-X	Red-orange	570	590
A11004	Alexa Fluor 568	Red-orange	578	603
A11005	Alexa Fluor 594	Red	590	617
T862	Texas Red	Red	595	615
T6390	Texas Red-X	Red	595	615
A21050	Alexa Fluor 633	Far-red	632	647
A31574	Alexa Fluor 635	Far-red	633	647
A10524	Cy5	Far-red	649	670
A865	Allophycocyanin	Far-red	650	660
A21000	Alexa Fluor 680–allophycocyanin	Far-red	650	702
A21006	Alexa Fluor 750–allophycocyanin	Infrared	650	775
A21235	Alexa Fluor 647	Far-red	650	668
A21054	Alexa Fluor 660	Far-red	663	690
A21057	Alexa Fluor 680	Far-red	679	702
A21036	Alexa Fluor 700	Infrared	702	723
A21037	Alexa Fluor 750	Infrared	749	775
A11375	Alexa Fluor 790	Infrared	782	805
B2763	Biotin	NA	<300	NA
G21060	Alkaline phosphatase	NA	NA	NA
G21040	Horseradish peroxidase	NA	NA	NA
G31567	β-Lactamase	NA	NA	NA
A10535	Unlabeled	NA	NA	NA
Goat Anti–Mouse IgG, Highly Cross-Adsorbed
A21049	Alexa Fluor 305	Blue	346	442
P31582	Pacific Blue	Blue	410	455
A11029 R37120 †	Alexa Fluor 488	Green	495	519
O11033	Oregon Green 488	Green	496	524
A11030	Alexa Fluor 546	Orange	556	573
A21424	Alexa Fluor 555	Orange	555	565
A11031	Alexa Fluor 568	Red-orange	578	603
A11032 R37121 †	Alexa Fluor 594	Red	590	617
A21052	Alexa Fluor 633	Far-red	632	647
A31575	Alexa Fluor 635	Far-red	633	647
A21236	Alexa Fluor 647	Far-red	650	668
A21055	Alexa Fluor 660	Far-red	663	690
A21058	Alexa Fluor 680	Far-red	679	702
A11357	Alexa Fluor 790	Infrared	782	805
D20691	DSB-X biotin	NA	<300	NA
Goat Anti–Mouse IgG, F(ab')₂ Fragments
A11068	Alexa Fluor 350	Blue	346	442
P31585	Pacific Orange	Orange	400	551
P31581	Pacific Blue	Blue	410	455
F11021	Fluorescein	Green	494	518
A11017	Alexa Fluor 488	Green	495	519
A11018	Alexa Fluor 546	Orange	556	573
A21425	Alexa Fluor 555	Orange	555	565
A10543	R-phycoerythrin (R-PE)	Orange	496, 546, 565	578
A11019	Alexa Fluor 568	Red-orange	578	603
A11020	Alexa Fluor 594	Red	590	617
A21053	Alexa Fluor 633	Far-red	632	647
A10539	Allophycocyanin	Far-red	650	660
A21237	Alexa Fluor 647	Far-red	650	668
A21059	Alexa Fluor 680	Far-red	679	702
B11027	Biotin	NA	<300	NA
F21452	Alkaline phosphatase	NA	NA	NA
F21453	Horseradish peroxidase	NA	NA	NA
A10534	Unlabeled	NA	NA	NA
Goat Anti–Mouse IgG, IgM
A10679	Fluorescein	Green	494	518
A10680	Alexa Fluor 488	Green	495	519
A10677	Horseradish peroxidase	NA	NA	NA
Goat Anti–Mouse IgG, IgM; F(ab')₂ Fragments
A10683	Fluorescein	Green	494	518
A10684	Alexa Fluor 488	Green	495	519
Goat Anti–Mouse IgG, IgM, IgA
A10667	Alexa Fluor 488	Green	495	519
A10676	Biotin	NA	<300	NA
A10668	Horseradish peroxidase	NA	NA	NA
A10666	Unlabeled	NA	NA	NA
Rabbit Anti–Mouse IgG
A21062	Alexa Fluor 350	Blue	346	442
A11059	Alexa Fluor 488	Green	495	519
A11060	Alexa Fluor 546	Orange	556	573
A21427	Alexa Fluor 555	Orange	555	565
A11061	Alexa Fluor 568	Red-orange	578	603
A11062	Alexa Fluor 594	Red	590	617
A21063	Alexa Fluor 633	Far-red	632	647
A21239	Alexa Fluor 647	Far-red	650	668
A21065	Alexa Fluor 680	Far-red	679	702
R21455	Horseradish peroxidase	NA	NA	NA
Rabbit Anti–Mouse IgG, F(ab')₂ Fragments
A21204	Alexa Fluor 488	Green	495	519
A21205	Alexa Fluor 594	Red	590	617
Chicken Anti–Mouse IgG
A21200	Alexa Fluor 488	Green	495	519
A21201	Alexa Fluor 594	Red	590	617
A21463	Alexa Fluor 647	Far-red	650	668
Donkey Anti–Mouse IgG
A10035	Alexa Fluor 350	Blue	346	442
A21202 R37114 †	Alexa Fluor 488	Green	495	519
A10036	Alexa Fluor 546	Orange	556	573
A31570	Alexa Fluor 555	Orange	555	565
A10037	Alexa Fluor 568	Red-orange	578	603
A21203 R37115 †	Alexa Fluor 594	Red	590	617
A31571	Alexa Fluor 647	Far-red	650	668
A10038	Alexa Fluor 680	Far-red	679	702
A11371	Alexa Fluor 790	Infrared	782	805
* Absorption (Abs) and fluorescence emission (Em) maxima of conjugates, in nm. † ReadyProbes ready-to-use imaging reagent. NA = Not applicable.

Table 7.1B Molecular Probes affinity-purified anti–rabbit IgG antibody conjugates.

Cat. No.	Label	Emission Color	Abs *	Em *
Goat Anti–Rabbit IgG
A11046	Alexa Fluor 350	Blue	346	442
M10992	Marina Blue	Blue	365	460
C2764	Cascade Blue	Blue	400	420
P31584	Pacific Orange	Orange	400	551
A31556	Alexa Fluor 405	Blue	402	421
P10994	Pacific Blue	Blue	410	455
A11064	Alexa Fluor 430	Yellow-green	434	539
F2765	Fluorescein	Green	494	518
A11008	Alexa Fluor 488	Green	495	519
O6381	Oregon Green 488	Green	496	524
B2766	BODIPY FL	Green	505	513
A31558	Alexa Fluor 514	Yellow-green	518	540
A11009	Alexa Fluor 532	Yellow	531	554
A10520	Cy3	Orange	552	570
T2769	Tetramethylrhodamine	Orange	555	580
A11010	Alexa Fluor 546	Orange	556	573
A21428	Alexa Fluor 555	Orange	555	565
P2771MP	R-phycoerythrin (R-PE)	Orange	496, 546, 565	578
A20981	Alexa Fluor 610–R-PE	Red	496, 546, 565	628
A20991	Alexa Fluor 647–R-PE	Far-red	496, 546, 565	668
A20984	Alexa Fluor 680–R-PE	Far-red	496, 546, 565	702
R6394	Rhodamine Red-X	Red-orange	570	590
A11011	Alexa Fluor 568	Red-orange	578	603
A11012	Alexa Fluor 594	Red	590	617
T2767	Texas Red	Red	595	615
T6391	Texas Red-X	Red	595	615
A21070	Alexa Fluor 633	Far-red	632	647
A31576	Alexa Fluor 635	Far-red	633	647
A10523	Cy5	Far-red	649	670
A10931	Allophycocyanin	Far-red	650	660
A21001MP	Alexa Fluor 680–allophycocyanin	Far-red	650	702
A21244	Alexa Fluor 647	Far-red	650	668
A21073	Alexa Fluor 660	Far-red	663	690
A21076	Alexa Fluor 680	Far-red	679	702
A21038	Alexa Fluor 700	Infrared	702	723
A21039	Alexa Fluor 750	Infrared	749	775
A11367	Alexa Fluor 790	Infrared	782	805
B2770	Biotin	NA	<300	NA
G21079	Alkaline phosphatase	NA	NA	NA
G21234	Horseradish peroxidase	NA	NA	NA
G31568	β-Lactamase	NA	NA	NA
A10533	Unlabeled	NA	NA	NA
Goat Anti–Rabbit IgG, Highly Cross-Adsorbed
A21068	Alexa Fluor 350	Blue	346	442
A11034 R37116 †	Alexa Fluor 488	Green	495	519
O11038	Oregon Green 488	Green	496	524
A11035	Alexa Fluor 546	Orange	556	573
A21429	Alexa Fluor 555	Orange	555	565
A11036	Alexa Fluor 568	Red-orange	578	603
A11037 R37117 †	Alexa Fluor 594	Red	590	617
A21071	Alexa Fluor 633	Far-red	632	647
A31577	Alexa Fluor 635	Far-red	633	647
A21245	Alexa Fluor 647	Far-red	650	668
A21074	Alexa Fluor 660	Far-red	663	690
A21109	Alexa Fluor 680	Far-red	679	702
A11369	Alexa Fluor 790	Infrared	782	805
Goat Anti–Rabbit IgG, F(ab')₂ Fragments
A11069	Alexa Fluor 350	Blue	346	442
A10526	Fluorescein	Green	494	518
A11070	Alexa Fluor 488	Green	495	519
A11071	Alexa Fluor 546	Orange	556	573
A21430	Alexa Fluor 555	Orange	555	565
A10542	R-phycoerythrin (R-PE)	Orange	496, 546, 565	578
A21069	Alexa Fluor 568	Red-orange	578	603
A11072	Alexa Fluor 594	Red	590	617
A21072	Alexa Fluor 633	Far-red	632	647
A21246	Alexa Fluor 647	Far-red	650	668
A21077	Alexa Fluor 680	Far-red	679	702
B21078	Biotin	NA	<300	NA
F21456	Alkaline phosphatase	NA	NA	NA
A10547	Horseradish peroxidase	NA	NA	NA
Chicken Anti–Rabbit IgG
A21441	Alexa Fluor 488	Green	495	519
A21442	Alexa Fluor 594	Red	590	617
A21443	Alexa Fluor 647	Far-red	650	668
Donkey Anti–Rabbit IgG
A10039	Alexa Fluor 350	Blue	346	442
A21206 R37118 †	Alexa Fluor 488	Green	495	519
A10040	Alexa Fluor 546	Orange	556	573
A31572	Alexa Fluor 555	Orange	555	565
A10042	Alexa Fluor 568	Red-orange	578	603
A21207 R37119 †	Alexa Fluor 594	Red	590	617
A31573	Alexa Fluor 647	Far-red	650	668
A10043	Alexa Fluor 680	Far-red	679	702
A11374	Alexa Fluor 790	Infrared	782	805
* Absorption (Abs) and fluorescence emission (Em) maxima of conjugates, in nm. † ReadyProbes ready-to-use imaging reagent. NA = Not applicable.

Table 7.1C Molecular Probes affinity-purified anti–rat IgG antibody conjugates.

Cat. No.	Label	Emission Color	Abs *	Em *
Goat Anti–Rat IgG
A21093	Alexa Fluor 350	Blue	346	442
A10528	Fluorescein	Green	494	518
A11006	Alexa Fluor 488	Green	495	519
O6382	Oregon Green 488	Green	496	524
A10522	Cy3	Orange	552	570
A10531	Tetramethylrhodamine	Orange	555	580
A11081	Alexa Fluor 546	Orange	556	573
A21434	Alexa Fluor 555	Orange	555	565
A10545	R-phycoerythrin (R-PE)	Orange	496, 546, 565	578
A11077	Alexa Fluor 568	Red-orange	578	603
A11007	Alexa Fluor 594	Red	590	617
T6392	Texas Red-X	Red	595	615
A21094	Alexa Fluor 633	Far-red	632	647
A10525	Cy5	Far-red	649	670
A10540	Allophycocyanin	Far-red	650	660
A21247	Alexa Fluor 647	Far-red	650	668
A21096	Alexa Fluor 680	Far-red	679	702
A10517	Biotin	NA	<300	NA
D20697	DSB-X biotin	NA	<300	NA
A10546	Alkaline phosphatase	NA	NA	NA
A10549	Horseradish peroxidase	NA	NA	NA
A10536	Unlabeled	NA	NA	NA
Goat Anti–Rat IgG, F(ab')₂ Fragments
A10527	Fluorescein	Green	494	518
A10544	R-phycoerythrin (R-PE)	Orange	496, 546, 565	578
A10691	Cy5–R-PE	Far-red	496, 546, 565	670
A10548	Horseradish peroxidase	NA	NA	NA
Rabbit Anti–Rat IgG
A21210	Alexa Fluor 488	Green	495	519
A21211	Alexa Fluor 594	Red	590	617
Chicken Anti–Rat IgG
A21470	Alexa Fluor 488	Green	495	519
A21471	Alexa Fluor 594	Red	590	617
A21472	Alexa Fluor 647	Far-red	650	668
Donkey Anti–Rat IgG
A21208	Alexa Fluor 488	Green	495	519
A21209	Alexa Fluor 594	Red	590	617
* Absorption (Abs) and fluorescence emission (Em) maxima of conjugates, in nm. NA = Not applicable.

Table 7.1D Molecular Probes affinity-purified anti–human IgG antibody conjugates.

Cat. No.	Label	Emission Color	Abs *	Em *
Goat Anti–Human IgG
A11013	Alexa Fluor 488	Green	495	519
A21089	Alexa Fluor 546	Orange	556	573
A21433	Alexa Fluor 555	Orange	555	565
A21090	Alexa Fluor 568	Red-orange	578	603
A11014	Alexa Fluor 594	Red	590	617
A21091	Alexa Fluor 633	Far-red	632	647
A21445	Alexa Fluor 647	Far-red	650	668
Mouse Anti–Human IgG₁
A10631	Alexa Fluor 488	Green	495	519
A10650	Biotin	NA	<300	NA
A10648	Horseradish peroxidase	NA	NA	NA
A10630	Unlabeled	NA	NA	NA
Mouse Anti–Human IgG₄
A10663	Biotin	NA	<300	NA
A10654	Horseradish peroxidase	NA	NA	NA
A10651	Unlabeled	NA	NA	NA
* Absorption (Abs) and fluorescence emission (Em) maxima of conjugates, in nm. NA = Not applicable.

Table 7.1E Molecular Probes affinity-purified anti–sheep IgG antibody conjugates.

Cat. No.	Label	Emission Color	Abs *	Em *
Donkey Anti–Sheep IgG
A21097	Alexa Fluor 350	Blue	346	442
A11015	Alexa Fluor 488	Green	495	519
A21098	Alexa Fluor 546	Orange	556	573
A21436	Alexa Fluor 555	Orange	555	565
A21099	Alexa Fluor 568	Red-orange	578	603
A11016	Alexa Fluor 594	Red	590	617
A21100	Alexa Fluor 633	Far-red	632	647
A21448	Alexa Fluor 647	Far-red	650	668
A21102	Alexa Fluor 680	Far-red	679	702
* Absorption (Abs) and fluorescence emission (Em) maxima of conjugates, in nm.

Table 7.1F Molecular Probes affinity-purified anti–goat IgG antibody conjugates.

Cat. No.	Label	Emission Color	Abs *	Em *
Rabbit Anti–Goat IgG
A10529	Fluorescein	Green	494	518
A11078	Alexa Fluor 488	Green	495	519
A10532	Tetramethylrhodamine	Orange	555	580
A21085	Alexa Fluor 546	Orange	556	573
A21431	Alexa Fluor 555	Orange	555	565
A11079	Alexa Fluor 568	Red-orange	578	603
A11080	Alexa Fluor 594	Red	590	617
A21086	Alexa Fluor 633	Far-red	632	647
A21446	Alexa Fluor 647	Far-red	650	668
A21088	Alexa Fluor 680	Far-red	679	702
A10518	Biotin	NA	<300	NA
R21458	Alkaline phosphatase	NA	NA	NA
R21459	Horseradish peroxidase	NA	NA	NA
A10537	Unlabeled	NA	NA	NA
Rabbit Anti–Goat IgG, F(ab')₂ Fragments
A21222	Alexa Fluor 488	Green	495	519
A21223	Alexa Fluor 594	Red	590	617
Chicken Anti–Goat IgG
A21467	Alexa Fluor 488	Green	495	519
A21468	Alexa Fluor 594	Red	590	617
A21469	Alexa Fluor 647	Far-red	650	668
Donkey Anti–Goat IgG
A21081	Alexa Fluor 350	Blue	346	442
A11055	Alexa Fluor 488	Green	495	519
A11056	Alexa Fluor 546	Orange	556	573
A21432	Alexa Fluor 555	Orange	555	565
A11057	Alexa Fluor 568	Red-orange	578	603
A11058	Alexa Fluor 594	Red	590	617
A21082	Alexa Fluor 633	Far-red	632	647
A21447	Alexa Fluor 647	Far-red	650	668
A21083	Alexa Fluor 660	Far-red	663	690
A21084	Alexa Fluor 680	Far-red	679	702
A11370	Alexa Fluor 790	Infrared	782	805
D20698	DSB-X biotin	NA	<300	NA
* Absorption (Abs) and fluorescence emission (Em) maxima of conjugates, in nm. NA = Not applicable.

Table 7.1G Molecular Probes affinity-purified anti–chicken IgG antibody conjugates.

Cat. No.	Label	Emission Color	Abs *	Em *
Goat Anti–Chicken IgG
A11039	Alexa Fluor 488	Green	495	519
A11040	Alexa Fluor 546	Orange	556	573
A21437	Alexa Fluor 555	Orange	555	565
A11041	Alexa Fluor 568	Red-orange	578	603
A11042	Alexa Fluor 594	Red	590	617
A21103	Alexa Fluor 633	Far-red	632	647
A21449	Alexa Fluor 647	Far-red	650	668
D20701	DSB-X biotin	NA	<300	NA
* Absorption (Abs) and fluorescence emission (Em) maxima of conjugates, in nm. NA = Not applicable.

Table 7.1H Molecular Probes affinity-purified anti–guinea pig IgG antibody conjugates.

Cat. No.	Label	Emission Color	Abs *	Em *
Goat Anti–Guinea Pig IgG
A11073	Alexa Fluor 488	Green	495	519
A11074	Alexa Fluor 546	Orange	556	573
A21435	Alexa Fluor 555	Orange	555	565
A11075	Alexa Fluor 568	Red-orange	578	603
A11076	Alexa Fluor 594	Red	590	617
A21105	Alexa Fluor 633	Far-red	632	647
A21450	Alexa Fluor 647	Far-red	650	668
* Absorption (Abs) and fluorescence emission (Em) maxima of conjugates in nm.

Table 7.1I Molecular Probes affinity-purified goat anti–hamster IgG antibody conjugates.

Cat. No.	Label	Emission Color	Abs *	Em *
Goat Anti–Hamster IgG
A21110	Alexa Fluor 488	Green	495	519
A21111	Alexa Fluor 546	Orange	556	573
A21112	Alexa Fluor 568	Red-orange	578	603
A21113	Alexa Fluor 594	Red	590	617
A21451	Alexa Fluor 647	Far-red	650	668
* Approximate absorption (Abs) and emission (Em) maxima of conjugates, in nm.

Table 7.1J Molecular Probes affinity-purified goat anti–IgM (µ chain) antibody conjugates.

Fluorophore	Abs/Em *	Anti–Mouse IgM †	Anti–Rat IgM †	Anti–Human IgM †
Alexa Fluor 350	346/442	A31552
Alexa Fluor 488	495/519	A21042	A21212	A21215
Alexa Fluor 546	556/573	A21045
Alexa Fluor 555	555/565	A21426
R-phycoerythrin	496, 546, 565/578	A10689 A10690 ‡
Alexa Fluor 568	578/603	A21043
Alexa Fluor 594	590/617	A21044	A21213	A21216
Alexa Fluor 633	632/647	A21046
Allophycocyanin	650/660	A10688
Alexa Fluor 647	650/668	A21238	A21248	A21249
Alexa Fluor 680	679/702	A21048
DSB-X biotin	NA	D20693
* Absorption (Abs) and fluorescence emission (Em) maxima of conjugates, in nm. † May also react with IgM from other species. ‡ F(ab')₂ fragments. NA = Not applicable.

For Research Use Only. Not for use in diagnostic procedures.

Summary of Molecular Probes Secondary Antibody Conjugates—Table 7.1

Learn more in The Molecular Probes® Handbook

Table 7.1 Summary of Molecular Probes secondary antibody conjugates.

Table 7.1A Molecular Probes affinity-purified anti–mouse IgG antibody conjugates.

Table 7.1B Molecular Probes affinity-purified anti–rabbit IgG antibody conjugates.

Table 7.1C Molecular Probes affinity-purified anti–rat IgG antibody conjugates.

Table 7.1D Molecular Probes affinity-purified anti–human IgG antibody conjugates.

Table 7.1E Molecular Probes affinity-purified anti–sheep IgG antibody conjugates.

Table 7.1F Molecular Probes affinity-purified anti–goat IgG antibody conjugates.

Table 7.1G Molecular Probes affinity-purified anti–chicken IgG antibody conjugates.

Table 7.1H Molecular Probes affinity-purified anti–guinea pig IgG antibody conjugates.

Table 7.1I Molecular Probes affinity-purified goat anti–hamster IgG antibody conjugates.

Table 7.1J Molecular Probes affinity-purified goat anti–IgM (µ chain) antibody conjugates.