Learn more in The Molecular Probes® Handbook
- Introduction to Amine Modification—Section 1.1
- Kits for Labeling Proteins and Nucleic Acids—Section 1.2
- Alexa Fluor Dyes Spanning the Visible and Infrared Spectrum—Section 1.3
- Fluorophores and Their Amine-Reactive Derivatives—Chapter 1
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Table 1.4 Alexa Fluor active esters and kits for labeling proteins and nucleic acids.
| Alexa Fluor Dye | Fluorescence Color (Abs/Em) * | Succinimidyl Ester (NHS Ester), TFP Ester or SDP Ester |
Kits for Labeling Proteins
| Kits for Labeling Nucleic Acids and Oligonucleotides | |||
|---|---|---|---|---|---|---|---|
| Protein Labeling Kits | Antibody Labeling Kits | Zenon Antibody Labeling Kits | |||||
| Alexa Fluor 350 | Blue (346/442) | A10168 | A10170 (P) | A20180 (Ab) | Z25000 (M IgG1) Z25100 (M IgG2a) Z25200 (M IgG2b) Z25300 (R IgG) Z25400 (H IgG) | ||
| Alexa Fluor 405 | Blue (402/421) | A30000 A30100 | Z25013 (M IgG1) Z25113 (M IgG2a) Z25213 (M IgG2b) Z25313 (R IgG) | ||||
| Alexa Fluor 430 | Yellow-green (434/539) | A10169 | A10171 (P) | Z25001 (M IgG1) Z25301 (R IgG) | |||
| Alexa Fluor 488 | Green (495/519) | A20000 † A20100 † A30052 (SDP) A30005 (TFP) A37563 (TFP) A37570 (TFP) | A10235 (P) ‡ A30006 (Micro) ‡ | A10468 (APEX) ‡ A20181 (Ab) ‡ | Z25002 (M IgG1) Z25090 (TSA) (M IgG1) Z25102 (M IgG2a) Z25202 (M IgG2b) Z25302 (R IgG) Z25602 (G IgG) Z25402 (H IgG) | U21650 (ULYSIS) A21665 (ARES) F32947 (FT) F32952 (FT) A20191 (Oligo) | |
| Alexa Fluor 514 | Yellow-green (518/540) | A30002 † | |||||
| Alexa Fluor 532 | Yellow (531/554) | A20001 A20101MP | A10236 (P) | A20182 (Ab) | Z25003 (M IgG1) Z25303 (R IgG) | U21651 (ULYSIS) | |
| Alexa Fluor 546 | Orange (556/573) | A20002 † A20102 † | A10237 (P) | A20183 (Ab) | Z25004 (M IgG1) Z25104 (M IgG2a) Z25204 (M IgG2b) Z25304 (R IgG) | U21652 (ULYSIS) A21667 (ARES) | |
| Alexa Fluor 555 | Red-orange (555/565) | A20009 A20109 A37564 A37571 | A20174 (P) A30007 (Micro) | A10470 (APEX) A20187 (Ab) | Z25005 (M IgG1) Z25105 (M IgG2a) Z25205 (M IgG2b) Z25305 (R IgG) Z25605 (G IgG) Z25405 (H IgG) | A21677 (ARES) F32948 (FT) F32953 (FT) | |
| Alexa Fluor 568 | Red-orange (578/603) | A20003 † A20103 † | A10238 (P) | A10494 (APEX) A20184 (Ab) | Z25006 (M IgG1) Z25106 (M IgG2a) Z25206 (M IgG2b) Z25306 (R IgG) Z25606 ( G IgG) | U21653 (ULYSIS) | |
| Alexa Fluor 594 | Red (590/617) | A20004 † A20104 † A37565 † A37572 † | A10239 (P) A30008 (Micro) | A10474 (APEX) A20185 (Ab) | Z25007 | U21654 (ULYSIS) A21669 (ARES) F32949 (FT) F32954 (FT) | |
| Alexa Fluor 610 | Red (612/628) | A30050 (X) | |||||
| Alexa Fluor 633 § | Far-red (632/647) | A20005 † A20105 † | A20170 (P) | ||||
| Alexa Fluor 647 § | Far-red (650/668) | A20006 A20106 A37566 A37573 | A20173 (P) A30009 (Micro) | A10475 (APEX) A20186 (Ab) S30044 (SAIVI) | Z25008 (M IgG1) Z25108 (M IgG2a) Z25208 (M IgG2b) Z25308 (R IgG) Z25608 (G IgG) Z25408 (H IgG) | U21660 (ULYSIS) A21676 (ARES) F32950 (FT) F32955 (FT) A20196 (Oligo) | |
| Alexa Fluor 660 § | Near-infrared (663/690) | A20007 | A20171 (P) | Z25009 (M IgG1) | U21656 (ULYSIS) A21671 (ARES) | ||
| Alexa Fluor 680 § | Near-infrared (679/702) | A20008 A20108 A37567 A37574 | A20172 (P) | A20188 (Ab) S30045 (SAIVI) | Z25010 (M IgG1) Z25110 (M IgG2a) Z25210 (M IgG2b) Z25310 (R IgG) | ||
| Alexa Fluor 700 § | Near-infrared (702/723) | A20010 A20110 | Z25011 (M IgG1) | ||||
| Alexa Fluor 750 § | Near-infrared (749/775) | A20011 A20111 A37568 A37575 | S30046 (SAIVI) | Z25312 (R IgG) | |||
| Alexa Fluor 790 § | Near-infrared (782/805) | A30051 A37569 | A20189 (Ab) | ||||
| * Approximate absorption (Abs) and fluorescence emission (Em) maxima for conjugates, in nm. † Mixed isomers. ‡ The APEX Alexa Fluor 488 Antibody Labeling Kits contain the Alexa Fluor 488 carboxylic acid SDP ester, whereas the other Alexa Fluor 488 protein labeling kits contain the amine-reactive Alexa Fluor 488 carboxylic acid TFP ester; the Alexa Fluor 488 kits for labeling nucleic acids and oligonucleotides contain the Alexa Fluor 488 succinimidyl ester. The Alexa Fluor Labeling Kits are described in detail in Kits for Labeling Proteins and Nucleic Acids—Section 1.2. § Human vision is insensitive to light beyond ~650 nm, and therefore it is not possible to view the far-red– and near-infrared–fluorescent dyes by looking through the eyepiece of a conventional fluorescence microscope. (Ab) = Antibody Labeling Kit. (APEX) = APEX Antibody Labeling Kit. (ARES) = ARES DNA Labeling Kit. (FT) = FISH Tag DNA Kit or FISH Tag RNA Kit. (G IgG) = Zenon Goat IgG Labeling Kit. (H IgG) = Zenon Human IgG Labeling Kit. (M IgG) = Zenon Mouse IgG Labeling Kit. (Micro) = Alexa Fluor Microscale Protein Labeling Kit. (Oligo) = Alexa Fluor Oligonucleotide Amine Labeling Kit. (P) = Easy-to-Use Protein Labeling Kit. (R IgG) = Zenon Rabbit IgG Labeling Kit. (SAIVI) = SAIVI Antibody Labeling Kit. (SDP) = Sulfodichlorophenol ester. (TFP) = Tetrafluorophenyl ester. (TSA) = Enhanced with TSA technology. (ULYSIS) = ULYSIS Nucleic Acid Labeling Kit. (X) = An aminohexanoyl spacer between the dye and the SE. | |||||||
For Research Use Only. Not for use in diagnostic procedures.