Table 19.7 Ca2+ affinities of BAPTA chelators.
| Chelator | Water-Soluble Salt | Cell-Permeant Ester | Kd for Ca2+ |
|---|---|---|---|
| BAPTA | B1204, B1212, B1214 | B1205, B6769 | No Mg2+: 160 nM * 1 mM Mg2+: 700 nM † |
| 5,5'-Dibromo BAPTA | D1211 | NA | No Mg2+: 1.6 µM ‡ |
| 5,5'-Difluoro BAPTA | NA | D1209 | No Mg2+: 635 nM § 1 mM Mg2+: 705 nM § |
| 5,5'-Dimethyl BAPTA | NA | D1207 | No Mg2+: 40 nM ‡ |
* Measured in 10 mM MOPS, 100 mM KCl, pH 7.2 at 22°C. † Measured in 10 mM HEPES, 300 mM KCl, pH 7.0 at 22°C. ‡ Measured in 10 mM MOPS, 100 mM KCl, pH 7.3 at 22°C. § Measured in 10 mM HEPES, 115 mM KCl, 20 mM NaCl, pH 7.05. NA = Not available. | |||
For Research Use Only. Not for use in diagnostic procedures.