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Table 1.2 Active esters and kits for labeling proteins and nucleic acids.

LabelFluorescence Color (Abs/Em) *Reactive** Dye or BiotinProtein Labeling KitsZenon Antibody Labeling KitsNucleic Acid Labeling Kits
Alexa Fluor 350Blue
(346/442)		A10168A20180 (Ab)
A10170 (P)		Z25000 (M IgG1)
Z25100 (M IgG2a)
Z25300 (R IgG)		 
Marina BlueBlue
(365/460)		M10165   
Pacific OrangeOrange
(400/551)		P30253P30014 (Ab)
P30016 (P)		Z25256 (M IgG1)
Z25257 (M IgG2a)		 
Alexa Fluor 405Blue
(402/421)		A30000
A30100		 Z25013 (M IgG1)
Z25113 (M IgG2a)
Z25213 (M IgG2b)
Z25313 (R IgG)		 
Pacific BlueBlue
(410/455)		P10163A10478 (APEX)
P30013 (Ab)
P30012 (P)		Z25041 (M IgG1)
Z25156 (M IgG2a)
Z25341 (R IgG)		 
Alexa Fluor 430Yellow-green
(434/539)		A10169A10171 (P)Z25001 (M IgG1) 
Fluorescein-EXGreen
(494/518)		F6130F10240 (P)
F6433 (F)		Z25042 (M IgG1)
Z25342 (R IgG)		C7604 (Nuc)
F32767 (Nuc)
FITCGreen
(494/518)		F143  (I)
F1906 (I)
F1907 (I)		F6434 (F)  
Alexa Fluor 488Green
(495/519)

A20000 
A20100 
A30052 (SDP)
A30005 (TFP)
A37563 (TFP)
A37570 (TFP)

A10468  (APEX)
A30006  (Micro)
A20181  (Ab)
A10235  (P)		Z25002 (M IgG1)
Z25090 (TSA) (M IgG1)
Z25102 (M IgG2a)
Z25202 (M IgG2b)
Z25302 (R IgG)
Z25602 (G IgG)
Z25402 (H IgG)		U21650 (ULYSIS)
A21665 (ARES)
F32947 (FT)
F32952 (FT)
A20191 (Oligo)
A32750 (Deca)
C11397 (Nuc)
C11403 (Nuc)

 

Oregon Green 488Green
(496/524)		O6080 (I) O6147
O6149
06185 (X)		A10476 (APEX)
O10241 (P)		  
pHrodo GreenGreen
(505/525)		P35369 (STP)   
Oregon Green 514Green
(511/530)		O6139   
Alexa Fluor 514Yellow-green
(518/540)		A30002    
Alexa Fluor 532Yellow
(531/554)		A20001
A20101MP		A20182 (Ab)
A10236 (P)		Z25003 (M IgG1)
Z25303 (R IgG)		U21651 (ULYSIS)
C11398 (Nuc)
Alexa Fluor 546Orange
(556/573)		A20002 
A20102 		A20183 (Ab)
A10237 (P)		Z25004 (M IgG1)
Z25104 (M IgG2a)
Z25204 (M IgG2b)
Z25304 (R IgG)

U21652 (ULYSIS)
A21667 (ARES)
C11401 (Nuc)
C11404 (Nuc)

Alexa Fluor 555Red-orange (555/565)A20009
A20109
A37564
A37571		A10470 (APEX)
A30007 (Micro)
A20187 (Ab)
A20174 (P)		Z25005 (M IgG1)
Z25105 (M IgG2a)
Z25205 (M IgG2b)
Z25305 (R IgG)
Z25605 (G IgG)		A21677 (ARES)
F32948 (FT)
A32762 (Nuc)
A32770 (Nuc)
A32755 (Deca)
A32756 (Deca)

 

Tetramethylrhodamine

and TAMRA

Red-orange (555/580)T1480 (I)
T1481 (I) C2211
C6123
C1171 
T6105  (X)		   
pHrodo RedRed-orange
(560/587)		P36600A10026 (PL)
P35363 (Micro)		  
Rhodamine RedRed-orange (570/590)R6160 (X)   
Alexa Fluor 568Red-orange (578/603)A20003 
A20103 		A10494 (APEX)
A20184 (Ab)
A10238 (P)		Z25006 (M IgG1)
Z25106 (M IgG2a)
Z25206 (M IgG2b)
Z25306 (R IgG)		C11399 (Nuc)
Alexa Fluor 594Red
(590/617)		A20004 
A20104 
A37565 
A37572 		A10474 (APEX)
A30008 (Micro)
A20185 (Ab)
A10239 (P)		Z25007 (M IgG1)
Z25107 (M IgG2a)
Z25207 (M IgG2b)
Z25307 (R IgG)
Z25607 (G IgG)
Z25407 (H IgG)

U21654 (ULYSIS)
A21669 (ARES)
F32949 (FT)
F32954 (FT)
C11400 (Nuc)

Texas RedRed
(595/615)		T6134  (X)
T20175 (X)		T10244 (P)Z25045 (M IgG1)C7631 (Nuc)
Alexa Fluor 610Red
(612/628)		A30050 (X)   
Alexa Fluor 633 §Far-red
(632/647)		A20005 
A20105 		A20170 (P)  
Alexa Fluor 635 §Far-red
(633/647)		    
Alexa Fluor 647 §Far-red
(650/668)		A20006
A20106
A37566
A37573		A10475 (APEX)
A30009 (Micro)
A20186 (Ab)
S30044 (SAIVI)
A20173 (P)		Z25008 (M IgG1)
Z25108 (M IgG2a)
Z25208 (M IgG2b)
Z25308 (R IgG)
Z25608 (G IgG)
Z25408 (H IgG)		U21660 (ULYSIS)
A21676 (ARES)
A20196 (Oligo)
A32763 (Nuc)
A32771 (Nuc)
A32757 (Deca)

 

Alexa Fluor 660 §Near-infrared
(663/690)		A20007A20171 (P)  
Alexa Fluor 680 §Near-infrared (679/702)A20008
A20108
A37567
A37574		A20188 (Ab)
S30045 (SAIVI)
A20172 (P)		Z25010 (M IgG1)
Z25110 (M IgG2a)
Z25210 (M IgG2b)
Z25310 (R IgG)		 
Alexa Fluor 700 §Near-infrared
(702/723)		A20010
A20110		 Z25011 (M IgG1) 
Alexa Fluor 750 §Near-infrared (749/775)A20011
A20111
A37568
A37575		S30046 (SAIVI)Z25312 (R IgG) 
Alexa Fluor 790 §Near-infrared (782/805)A30051
A37569		A20189 (Ab)  
BiotinNA

B1513
B1582 (X)
B1606 (XX)
B6353 (S)(X)
B6352 (S)(XX)

A10495 (APEX)
B30010 (Micro)
B30756 (Micro)
F6347 (F)		Z25052 (M IgG1)
Z25152 (M IgG2a)
Z25252 (M IgG2b)
Z25352 (R IgG)
Z25452 (H IgG)		 
DNP–biotinNA (364/none)B2604 (X)   
DSB-X biotinNA D20655 (P)  
* Approximate absorption (Abs) and fluorescence emission (Em) maxima for conjugates, in nm. ** Reactive Dye or Biotin has the succinimidyl ester (SE, NHS ester) group unless noted otherwise. § Human vision is insensitive to light beyond ~650 nm, and therefore it is not possible to view the far-red– and near-infrared–fluorescent dyes by looking through the eyepiece of a conventional fluorescence microscope. NA = Not applicable.

(Ab) = Antibody Labeling Kit
(APEX) = APEX™ Antibody Labeling Kit.
(ARES) = ARES™ DNA Labeling Kit.
(Deca) = Alexa Fluor® decapack, for microarrays.
(F) = FluoReporter Protein Labeling Kit.
(FT) = FISH Tag DNA Kit or FISH Tag RNA Kit.
(G IgG) = Zenon® Goat IgG Labeling Kit.
(H IgG) = Zenon® Human IgG Labeling Kit.
(I) = Isothiocyanate
(M IgG) = Zenon® Mouse IgG Labeling Kit.
(Micro) = Microscale Protein Labeling Kit.
(Nuc) = Nucleotides
(Oligo) = Alexa Fluor® Oligonucleotide Amine Labeling Kit.
(P) = Protein Labeling Kit.
(PL) = pHrodo™ Labeling Kit
(R IgG) = Zenon® Rabbit IgG Labeling Kit.
(S) = Sulfosuccinimidyl ester
(SAIVI) = SAIVI™ Antibody Labeling Kit.
(SDP) = Sulfodichlorophenol ester.
(STP) =  Sulfotetrafluorophenol ester
(TFP) = Tetrafluorophenyl ester.
(TSA) = Enhanced with TSA™ technology.
(ULYSIS) = ULYSIS® Nucleic Acid Labeling Kit.
(X) = An aminohexanoyl spacer between the dye and the SE.

(XX) = Two aminohexanoyl linkers conjugated to provide a 14-atom spacer between the dye and SE.

For Research Use Only. Not for use in diagnostic procedures.