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Table 8.7 Amine-reactive dyes for nucleic acid sequencing.
| Cat. No. | Reactive Dye * | Ex/Em § | Handbook Location |
|---|---|---|---|
| C2210 | 5-FAM, SE † | 494/518 | Fluorescein, Oregon Green and Rhodamine Green Dyes—Section 1.5 |
| C6164 | 6-FAM, SE | 494/518 | Fluorescein, Oregon Green and Rhodamine Green Dyes—Section 1.5 |
| C6127 | 5-CR 6G, SE | 525/555 | Long-Wavelength Rhodamines, Texas Red Dyes and QSY Quenchers—Section 1.6 |
| C6171MP | 6-JOE, SE † | 522/550 | Fluorescein, Oregon Green and Rhodamine Green Dyes—Section 1.5 |
| C2211 | 5-TAMRA, SE | 555/580 | Long-Wavelength Rhodamines, Texas Red Dyes and QSY Quenchers—Section 1.6 |
| C6123 | 6-TAMRA, SE † | 555/580 | Long-Wavelength Rhodamines, Texas Red Dyes and QSY Quenchers—Section 1.6 |
| C6125 | 5-ROX, SE | 580/605 | Long-Wavelength Rhodamines, Texas Red Dyes and QSY Quenchers—Section 1.6 |
| C6126 | 6-ROX, SE † | 580/605 | Long-Wavelength Rhodamines, Texas Red Dyes and QSY Quenchers—Section 1.6 |
| D2184 | BODIPY FL, SE ‡ | 505/513 | BODIPY Dye Series—Section 1.4 |
| D6140 | BODIPY FL, SSE | 505/513 | BODIPY Dye Series—Section 1.4 |
| D6102 | BODIPY FL-X, SE | 505/513 | BODIPY Dye Series—Section 1.4 |
| D6180 | BODIPY R6G, SE ‡ | 528/550 | BODIPY Dye Series—Section 1.4 |
| D6117 | BODIPY TMR-X, SE | 542/574 | BODIPY Dye Series—Section 1.4 |
| D2222 | BODIPY 564/570, SE ‡ | 565/571 | BODIPY Dye Series—Section 1.4 |
| D2228 | BODIPY 581/591, SE ‡ | 584/592 | BODIPY Dye Series—Section 1.4 |
| D6116 | BODIPY TR-X, SE | 589/617 | BODIPY Dye Series—Section 1.4 |
* FAM = carboxyfluorescein; CR 6G = carboxyrhodamine 6G; JOE = 6-carboxy-4',5'-dichloro-2',7'-dimethoxyfluorescein; TAMRA = carboxytetramethylrhodamine; ROX = carboxy-X-rhodamine; BODIPY = a substituted 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene derivative; SE = succinimidyl ester; SSE = water-soluble sulfosuccinimidyl ester. † These are the most widely used isomers for DNA sequencing. ‡ These BODIPY derivatives were reported to be useful for automated DNA sequencing, in part because the dyes have a minimal effect on the mobility of the fragment during electrophoresis and also exhibit well-resolved spectra with narrow bandwidths. § Excitation (Ex) and emission (Em) maxima in nm. | |||
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