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Table 7.9 Molecular Probes avidin, streptavidin, NeutrAvidin and CaptAvidin conjugates.
| Label (Abs/Em) * | Streptavidin | NeutrAvidin | Avidin | CaptAvidin |
|---|---|---|---|---|
| Fluorophore Conjugates | ||||
| Alexa Fluor 350 (346/442) | S11249 | A11236 | ||
| Marina Blue (365/460) | S11221 | |||
| Cascade Blue (400/420) | A2663 | |||
| Alexa Fluor 405 (402/421) | S32351 | |||
| Pacific Orange (400/551) | S32365 | |||
| Cascade Yellow (402/545) | S11228 | |||
| Pacific Blue (410/455) | S11222 | |||
| Pacific Green (411/500) | S11200 | |||
| Alexa Fluor 430 (434/539) | S11237 | |||
| Fluorescein (494/518) | S869 | A2662 | A821 | |
| Alexa Fluor 488 (495/519) | S11223, S32354 † | A21370 | ||
| Oregon Green 488 (496/524) | S6368 | A6374 | ||
| R-Phycoerythrin (496/578) ‡ | S866, S21388 § | A2660 | ||
| Alexa Fluor 610–R-PE (496/628) ‡ | S20982 | |||
| Alexa Fluor 647–R-PE (496/668) ‡ | S20992 | |||
| Alexa Fluor 680–R-PE (496/702) ‡ | S20985 | |||
| Alexa Fluor 750–R-PE (496/775) ‡ | S32363 | |||
| Oregon Green 514 (511/530) | S6369 | |||
| Alexa Fluor 514 (518/540) | S32353 | |||
| Alexa Fluor 532 (531/554) | S11224 | |||
| B-Phycoerythrin (546/575) ‡ | S32350 | |||
| Tetramethylrhodamine (555/580) | S870 | A6373 | ||
| Alexa Fluor 546 (556/573) | S11225 | |||
| Alexa Fluor 555 (555/565) | S21381, S32355 † | |||
| Rhodamine B (570/590) | S871 | |||
| Rhodamine Red-X (570/590) | S6366 | A6378 | ||
| Alexa Fluor 568 (578/603) | S11226 | |||
| Alexa Fluor 594 (590/617) | S11227, S32356 † | |||
| Texas Red (595/615) | S872 | A2665 | A820 | |
| Texas Red-X (595/615) | S6370 | |||
| Alexa Fluor 633 (632/647) | S21375 | |||
| Alexa Fluor 635 (633/647) | S32364 | |||
| Alexa Fluor 647 (650/668) | S21374, S32357 † | |||
| Allophycocyanin (650/660) | S868, S32362 § | |||
| Alexa Fluor 680–allophycocyanin (650/702) | S21002 | |||
| Alexa Fluor 700–allophycocyanin (650/723) | S21005 | |||
| Alexa Fluor 750–allophycocyanin (650/775) | S21008 | |||
| Alexa Fluor 660 (663/690) | S21377 | |||
| Alexa Fluor 680 (679/702) | S21378, S32358 † | |||
| Alexa Fluor 700 (702/723) | S21383 | |||
| Alexa Fluor 750 (749/775) | S21384 | |||
| Alexa Fluor 790 (782/805) | S11378 | |||
| Qdot Nanocrystal Conjugates | ||||
| Qdot 525 (405/525) | Q10141MP ** | |||
| Qdot 565 (405/565) | Q10131MP ** | |||
| Qdot 585 (405/585) | Q10111MP ** | |||
| Qdot 605 (405/605) | Q10101MP ** | |||
| Qdot 625 (405/625) | Q22063 ** A10196 ** | |||
| Qdot 655 (405/655) | Q10121MP ** | |||
| Qdot 705 (405/705) | Q10161MP ** | |||
| Qdot 800 (405/800) | Q10171MP ** | |||
| FluoSpheres and TransFluoSpheres Microsphere Conjugates | ||||
| Europium luminescent FluoSpheres microspheres †† (365/610) | F20883, F20884 | |||
| Yellow-green–fluorescent FluoSpheres microspheres †† (505/515) | F8780 | F8771, F8774, F8776 | ||
| Red-fluorescent FluoSpheres microspheres †† (580/605) | F8770, F8775 | |||
| TransFluoSpheres microspheres †† (488/605) | T8860, T8861 | |||
| TransFluoSpheres microspheres †† (488/645) | T10711 | |||
| Nonfluorescent FluoSpheres microspheres †† | F8772, F8777 | |||
| Gold Conjugates | ||||
| NANOGOLD | N24918 | |||
| Alexa Fluor 488 FluoroNanogold (495/519) | A24926 | |||
| Alexa Fluor 488 colloidal gold (495/519) | A32360 ‡‡, A32361 §§ | |||
| Alexa Fluor 594 FluoroNanogold (590/617) | A24927 | |||
| Enzyme Conjugates | ||||
| Alkaline phosphatase | S921 | |||
| β-Galactosidase | S931 | |||
| Horseradish peroxidase | S911 | A2664 | ||
| β-Lactamase | S31569 | |||
| Acrylamide and Agarose Conjugates | ||||
| Acrylamide | S21379 | |||
| Agarose | S951 | C21386 | ||
| Unlabeled Avidins | ||||
| Unlabeled | S888 | A2666 | A887, A2667 | C21385 |
| * Approximate absorption (Abs) and fluorescence emission (Em) maxima, in nm, for conjugates. † These Alexa Fluor streptavidin conjugates are supplied in 0.5 mL units of a 2 mg/mL solution. ‡ Multiple absorption peaks. § Premium grade. ** Properties and applications of Qdot nanocrystal conjugates are described in Qdot Nanocrystals—Section 6.6. The Qdot Streptavidin Sampler Kit (Q10151MP) contains six different Qdot streptavidin conjugates. †† FluoSpheres and TransFluoSpheres microspheres are available in different diameters, including 0.04 µm, 0.2 µm and 1.0 µm. ‡‡ 5 nm colloidal gold conjugate. §§ 10 nm colloidal gold conjugate. | ||||
For Research Use Only. Not for use in diagnostic procedures.