Learn more in The Molecular Probes® Handbook
- Alexa Fluor Dyes Spanning the Visible and Infrared Spectrum—Section 1.3
- Fluorophores and Their Amine-Reactive Derivatives—Chapter 1
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Table 1.6 R0 values for some Alexa Fluor dyes.
| Donor |
Acceptor
| |||||
|---|---|---|---|---|---|---|
| Alexa Fluor 488 | Alexa Fluor 546 | Alexa Fluor 555 | Alexa Fluor 568 | Alexa Fluor 594 | Alexa Fluor 647 | |
| Alexa Fluor 350 | 50 | |||||
| Alexa Fluor 488 | NA | 64 | 70 | 62 | 60 | 56 |
| Alexa Fluor 546 | NA | 70 | 71 | 74 | ||
| Alexa Fluor 555 | NA | 47 | 51 | |||
| Alexa Fluor 568 | NA | 82 | ||||
| Alexa Fluor 594 | NA | 85 | ||||
| Alexa Fluor 647 | NA | |||||
| R0 values in angstroms (Å) represent the distance at which fluorescence resonance energy transfer from the donor dye to the acceptor dye is 50% efficient (Förster radius). Values were calculated from spectroscopic data as outlined (Fluorescence Resonance Energy Transfer (FRET)—Note 1.2). NA = Not applicable. | ||||||
For Research Use Only. Not for use in diagnostic procedures.