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- Innovations
- Workflow
- Resources
- Extraction
- Quantitation
- Enzyme Selection
- Thermal Cycling
- Analysis
Ready-to-use magnetic beads magnetic beads designed for PCR cleanup, effectively removing small fragments like dNTPs, salts, primers, and primer-dimers.
These beads offer consistent binding capacity and highly reproducible results. Features include:
- 90% recovery rate for DNA fragments larger than 90 bp
- Flexible starting sample volumes and scalability
- Compatible with both manual and automated processes
- Can be stored at room temperature.
Applied Biosystems™ MagMAX™ Sequential DNA/RNA Kit
The Applied Biosystems MagMAX Sequential DNA/RNA kit utilizes the power of Dynabeads magnetic bead technology to enable the sequential purification of high-quality genomic DNA and total RNA from a single whole blood or bone marrow sample for your cancer research.
Maximize DNA and RNA yields from precious samples with the following features:
- Separate ready-to-use elutes for DNA and RNA
- Compatible with KingFisher sample purification systems for a scalable workflow
- Ideal for cancer research, offering insights into genetic alterations relevant to blood cancers.
MagMAX™ Prime Viral/Pathogen NA Isolation Kit
The MagMAX Prime Viral/Pathogen NA Isolation Kit is a magnetic-bead-based nucleic acid purification kit that allows one nucleic acid extraction kit to be used for multiple microbiological organisms and infectious disease research applications.
- Designed to isolate and purify microbial nucleic acid from a range of biological samples such as swabs in transport media, saliva, stool, urine, plasma, and serum
- Flexible workflow allowing various sample types to be batched on a single plate
- Flexible protocol accommodates sample volume inputs of 200–400 μL
- Fast automated KingFisher Flex, KingFisher Apex, KingFisher Apex Dx (RUO mode) scripts to allow for 96 samples to be processed in less than 60 minutes
SuperScript IV UniPrime One-Step RT-PCR System
Our latest one-step formulation for performing fast cDNA synthesis and PCR amplification in a single tube.
- Highest performance with SuperScript IV reverse transcriptase and modified Platinum SuperFi II DNA polymerase
- Universal annealing temperature
- Visual tracking with colored reagents for improved confidence
- Supports multiplex RT-PCR
Invitrogen E-Gel Power Snap Electrophoresis Systems
E-Gel Power Snap Systems are compact benchtop instruments that have integrated nucleic acid electrophoresis and gel imaging functionalities.
- Real-time visualization: Amber filter to monitor real-time sample migration
- Sample safety: Blue-light transilluminator does not damage DNA samples
- Flexible throughput: Gels with 11-, 22-, 48-, and 96-wells can be run (model-dependent)
- High resolution camera for image capture, with image editing capabilities
Choosing the right thermal cycler is key to the success of your PCR experiments across applications. Applied Biosystems thermal cyclers help generate precise, consistent, reproducible results for every challenge, experiment, and budget. VeritiPro thermal cycler offers:
- 96- and 384-well options with max block ramp rate of 6.0°C/sec
- VeriFlex technology for precise temperature control
- Easy method transfer from old thermal cyclers with simulation modes
- Remote access with Thermo Fisher Connect
Platinum II Taq Hot-Start DNA Polymerase
Our latest one-step formulation for performing fast cDNA synthesis and PCR amplification in a single tube.
- Universal primer annealing at 60°C
- 4x faster DNA synthesis and inhibitor resistance
- Platinum hot-start technology - superior specificity, sensitivity, yields and room temperature reaction setup
- Green buffer formats - helps reduce pipetting errors with direct gel loading
Get Ready for the Future with Molecular Biology Innovations
Elevate your scientific journey with tools for DNA & RNA isolation, protein extraction, reverse transcription, amplification, cloning, and nucleic acid electrophoresis. Uncover innovation for accurate results.
Learn More
Technical note- Qubit 1X dsDNA assays
The Invitrogen™ Qubit™ 1X assays were developed To further simplify the nucleic acid quantification workflow, The 1X formulations enable users to simply combine their DNA samples with the provided 1X working solution without the need for assay preparation. Cownload the technical note to know more.
Download now
Application note- Qubit RNA IQ Assay
The Qubit RNA IQ Assay is a fast and easy method to measure RNA quality in under 5 minutes on the Qubit 4 or Qubit Flex Fluorometer. Check this application note to know more.
Download now
PCR and reverse transcription enzymes selection guide
The ultimate compilation of enzymes for your RT and PCR experiments.
Download now
DNA & RNA Extraction Articles, Webinars, and Videos
Read scientific articles, listen to webinars and watch videos on foundational life science topics and modern research applications.
Learn More
TRIzol™ Reagent is a complete, ready-to-use reagent for the isolation of high-quality total RNA or the simultaneous isolation of RNA, DNA, and protein from a variety of biological samples.
These beads offer consistent binding capacity and highly reproducible results.
Features include:
- Permits the isolation of RNA, DNA, and protein from the same sample
- Offers superior lysis capability, even with difficult sample types
- Optimized formulations and protocols for tissues, cells, serum, virus, and bacteria
Column-based kit used to isolate more RNA than competitor mini spin columns in 20 mins.
Features include:
- Isolate more RNA than competitor mini spin columns: up to 1000 μg of total RNA from one column
- Flexible–optional on-column or post-isolation DNase treatment.
- Special RNase-free reagents combined with certified RNase-free silica membranes in a unique column configuration without the need of hazardous phenol/chloroform extraction, CsCl centrifugation, or LiCl or alcohol precipitation.
Dynabeads™ mRNA DIRECT™ Purification Kit
Designed for simple and rapid isolation of pure, intact polyadenylated (polyA) mRNA directly from the crude lysate of animal and plant cells and tissues.
Features include:
- Fast—15 minute procedure yields pure, intact mRNA
- Highly pure mRNA isolation—best choice upstream of cDNA synthesis
- Sensitive mRNA isolation—enables cDNA synthesis and cDNA library construction from ultra-small starting samples
MagMAX™ mirVana™ Total RNA Isolation Kit
Isolation of total RNA, including small rnas such as microRNAs, from cells, plasma, serum, tissue, urine, whole blood.
Features include:
- Automation-ready, phenol-free extraction
- Streamlined protocols for cells, plasma, serum, tissue, urine, whole blood.
- Recovery of pure miRNAs compatible with TaqMan™ Advanced miRNA cDNA Synthesis Kit and TaqMan™ Advanced miRNA Assays
MagMAX™ DNA Multi-Sample Ultra 2.0 Kit
Process a broad range of sample volume inputs simultaneously without sample normalization or reagent volume adjustments for hassle-free sample prep.
Features include of MagMAX DNA Multi-Sample Ultra 2.0 Kit include:
- Process 6–96 samples in 45 minutes with 5 minutes hands-on time
- Automation-friendly stand-alone MagMAX DNA Multi-Sample Ultra 2.0 reagent bottle, KingFisher™ Ready DNA Ultra 2.0 Prefilled Plates and KingFisher™ Duo-Ready DNA Ultra 2.0 Prefilled Plates
- New release scripts support 10 ml blood and 400 ul bone marrow for biobanking.
KingFisher Sample Purification Systems
The Thermo Scientific KingFisher purification system is the automated purification for DNA, RNA, proteins, and cells.
Features include:
- KingFisher systems can gently and efficiently isolate and purify nucleic acids, proteins, and cells from a range of sample types for a wide variety of downstream applications.
- Choice of throughputs - process 6 to 96 samples per run depending on the model
- Protocol customization - easily edit, modify, or create new protocols with BindIt software
Simplify your DNA, RNA, and protein quantification with the Invitrogen™ Qubit™ 4Fluorometer and Qubit™ assays. Experience the simplicity of the Qubit 4 Fluorometer, with a touch screen and preprogrammed protocols specifically designed to couple with high-performing Qubit assays.
- Sensitive—extremely useful for diluted or low-abundance samples
- Specific—quantifies only the molecule of choice: DNA, RNA, or protein
- Accurate—quantify across a broad range with high reproducibility
- Tolerant—common contaminants are well-tolerated by assays
DNA polymerase is an essential component for PCR because of its key role in synthesizing new DNA strands. Because of the sensitive and specific nature of PCR, it is important to choose high-quality enzymes and reagents to produce optimal results.
Download the free selection guide to find the best enzyme for PCR and RT applications.
For hot-start PCR
Platinum II Taq Hot-Start DNA Polymerase - Higher inhibitor tolerance and faster DNA synthesis than standard Taq enzyme
For high-fidelity PCR
Platinum SuperFi II DNA Polymerase - 300x more accurate than Taq DNA polymerase
For direct PCR
Platinum Direct PCR Universal Master Mix - Direct amplification from samples without genomic DNA purification
Thermal cyclers
Applied Biosystems thermal cyclers enable precise, consistent results for every challenge, application, and budget. Since 1987, our instruments have built a reputation for reliability, temperature accuracy, user-friendly interfaces, and innovation.
Deciding on a thermal cycler for your lab? Request a quote
PCR plastics
Using the right PCR plastics for your application and instrument can improve the reliability of your PCR results. Regardless of the plastics format you select, proper fit and uniform heat transfer during thermal cycling are essential for successful PCR.
To find plastics compatible with Applied Biosystems and other thermal cyclers, use our PCR Plastics Selection Tool
Verification by Nucleic Acid Electrophoresis
Nucleic acid electrophoresis is a common technique in molecular biology to separate, identify, quantify, and/or purify nucleic acids. Traditionally, setting up electrophoresis involves numerous steps to achieve optimal separation and analysis of nucleic acid samples, such as gel preparation, ladder selection, sample visualization, and gel documentation. Now with the all-in-one, bufferless E-Gel Power Snap electrophoresis systems, save time and simplify your work—run, visualize, and image on one integrated device.
E-Gel Power Snap Plus Electrophoresis System
E-Gel Power Snap Plus Electrophoresis System combines the convenience of quick real-time nucleic acid analysis with high-quality visualization in both high and low-throughput E-gels. Get results in as little as 15 minutes. No need to pour buffers or your own gels—Power Snap Plus is an integrated system to run, visualize and image gels as a dry, precast bufferless system!
With E-Gel precast gels, no need to spend valuable time preparing gels. E-gels are self-contained and ready to use with agarose, electrodes, and DNA stains (Invitrogen SYBR Safe or SYBR Gold gel stains). They are packaged inside a disposable, UV-transparent cassette and can be used to analyze both DNA and RNA.
- Innovations
- Applications
- Resources
- Gene Expression
- Mutation Detection
- Chromosomal Aberrations
- SNP Genotyping
- Quantitation
QuantStudio Absolute Q Digital PCR System
The Applied Biosystems QuantStudio Absolute Q Digital PCR System is a plate-based digital PCR (dPCR) platform powered by proprietary microfluidic array plate (MAP) technology. that enables all the necessary steps for dPCR—compartmentalizing, thermal cycling, and data acquisition—to be conducted on a single instrument. The dPCR workflow is identical to the qPCR workflow you are familiar with to improve ease of use, minimize hands on steps, and maximize consistency.
- Single instrument. Single plate. Simple workflow
- Minimize wasted sample volume
- Fast time to answer
- MAP16 dPCR plates enable exceptional consistency
TaqMan™ Cells-to-CT™ Express Kit
The TaqMan Cells-to-CT Express Kit allows for rapid gene expression analysis directly from cultured cells without the need for RNA purification. Now with an even more simplified workflow and high sensitivity, this kit enables two-step RT-qPCR and provides gene expression analysis results in approximately 70 minutes.
- Rapid and easy workflow, with samples ready for RT-qPCR in as little as 5 minutes
- No RNA purification required, eliminating the need for columns, heating, or centrifugation
- Verified workflows with optimized reagents for efficient performance out of the box
TaqPath™ DuraPlex™ 1-Step RT-qPCR Master Mix
Automation-ready, single-tube reagent optimized for highly sensitive pathogen detection even in the presence of inhibitors. This benchtop-stable formulation can multiplex 6 targets in a single well and is approved for use in laboratories running or developing high-throughput molecular diagnostic assays.
- Support workflow automation
- Optimized for high-order multiplexing
- Improved handling, including lower viscosity
- Robust performance even in the presence of substances that can inhibit PCR
GeneTitan™ MC Fast Scan Instrument
The Applied Biosystems GeneTitan MC Fast Scan Instrument and proven GeneChip array using Axiom 2.0 assay are the first to provide a hands-free, automated solution for genome-wide SNP genotyping and copy number variation analysis in human and agrigenomics.
Features of the GeneTitan MC Fast Scan Instrument include:
- Fast
- Scalable
- Flexible
- Accurate
- Adaptable
The Axiom™ PangenomiX Array brings the most ethnically diverse genomic coverage in a high throughput format array to researchers’ fingertips. This human genotyping research array was mindfully designed for whole genome imputation across global populations to enable scientists to create diverse genomic datasets that have traditionally been built on Caucasian cohorts. It is an essential research tool in human genomics, including applications such as genome wide association studies (GWAS), population health initiatives, polygenic risk scores, and drug discovery.
Dynabeads Streptavidin for Target Enrichment
Dynabeads Streptavidin for Target Enrichment beads are 1-μm monosized, monodispersed, superparamagnetic beads covalently coupled to streptavidin and intended for enrichment of target sequences for library preparations for next-generation sequencing (NGS) workflows.
Benefits of Dynabeads Streptavidin for Target Enrichment beads include:
- Performance—high sensitivity and reproducibility of results
- Efficiency—fast binding kinetics and high binding capacity
- Accuracy—excellent coverage, which enables high confidence in variant detection
- Automation ready—easy automation for high-throughput needs on the KingFisher Purification System
Ready-to-use magnetic beads magnetic beads designed for PCR cleanup, effectively removing small fragments like dNTPs, salts, primers, and primer-dimers.
These beads offer consistent binding capacity and highly reproducible results. Features include:
- 90% recovery rate for DNA fragments larger than 90 bp
- Flexible starting sample volumes and scalability
- Compatible with both manual and automated processes
- Can be stored at room temperature
SeqStudio™ Flex Genetic Analyzers
The Applied Biosystems SeqStudio Flex Series genetic analyzers deliver the high level of Sanger sequencing and fragment analysis data quality and reliable performance that scientists need, while leveraging technological advances for increased flexibility and easier operation.
- Schedule Flexibility: Four-plate capacity, continuous plate loading, and urgent sample reprioritization
- Ease of use: Easier capillary array installation, streamlined one-button start up, autocalibration, integrated touchscreen computer with intuitive software
- Advanced communications and connectivity: Remote setup, monitoring, and data transfer
Technical note- Qubit 1X dsDNA assays
The Invitrogen™ Qubit™ 1X assays were developed To further simplify the nucleic acid quantification workflow, The 1X formulations enable users to simply combine their DNA samples with the provided 1X working solution without the need for assay preparation. Cownload the technical note to know more.
Download now
Application note- Qubit RNA IQ Assay
The Qubit RNA IQ Assay is a fast and easy method to measure RNA quality in under 5 minutes on the Qubit 4 or Qubit Flex Fluorometer. Check this application note to know more.
Download now
Flyer - GeneTitan MC Fast Scan Instrument
The GeneTitan MC Fast Scan Instrument is a fully integrated
solution for automated microarray research genotyping—now
with enhanced scanning speed and throughput.
Download now
Seq it out video series
Get bite-sized answers to your everyday Sanger sequencing and fragment analysis questions
Learn more
Sanger sequencing workflow brochure
Sanger sequencing is the gold-standard technology for accurate sequence determination with single-base resolution. Ideal for targeted sequencing and confirmation analysis.
Free download
Real-Time PCR learning center
Whether you are new to real-time PCR or an experienced user looking to learn new tricks, we have a library of educational material to help you.
Learn more
Application guide
Fluorescent capillary electrophoresis (CE) is a genomic analysis method that separates fluorescently labeled DNA fragments based on size,and is the foundation of Sanger sequencing and fragment analysis. Check this application guide to know more.
Free download
Explore our comprehensive gene expression analysis solutions and technologies
Gene expression analysis by Real-time PCR
Real-time PCR Reagents
Applied Biosystems™ TaqMan™ Fast Advanced Master Mix
TaqMan Fast Advanced Master Mix delivers accurate quantification and dependability in less time on an array of qPCR instrument platforms.
Applied Biosystems™ TaqMan™ Fast Virus 1-Step Master Mix
TaqMan Fast Virus 1-Step Master Mix is designed for fast, highly sensitive real-time RT-PCR even in the presence of challenging PCR inhibitors.
Applied Biosystems™ PowerTrack™ SYBR Green Master Mix
TaqMan Fast Virus 1-Step Master Mix is designed for fast, highly sensitive real-time RT-PCR even in the presence of challenging PCR inhibitors.
Real-time PCR Assays
- Detect virtually any gene product with our predesigned assays
- Gold-standard specificity, sensitivity, and reproducibility
- Best-coverage assays to detect highest number of transcript variants possible
cDNA synthesis for gene expression assays
SuperScript™ IV VILO™ Master Mix
The cDNA synthesis master mix for accurate gene quantification.
SuperScript IV VILO Master Mix contains a proprietary helper protein which improves the interaction between SuperScript IV Reverse Transcriptase and the template RNA, and thereby, extends linearity across ten orders of magnitude for input RNA.
This means that you can normalize your lower-abundance genes to your reference genes without worrying about potential variation of RT efficiency at different RNA input levels.
Sanger sequencing for genotyping
PCR cleanup reagents
ExoSAP-IT Express reagent: fastest PCR cleanup method
Accelerate result accuracy with confidence with ExoSAP-IT Express reagent. Our one-step solution that delivers higher yields and full PCR product recovery.
- Five-minute protocol—fast turnaround time
- One-tube, one-step PCR cleanup— add reagent directly to PCR product
- Novel enzyme technology— irreversibly inactivated in just one minute at 80°C
- Complete sample recovery—100% recovery of PCR products, regardless of amplicon length
- Eliminates spin columns or beads—helps to significantly decrease time and errors while increasing yield
Sequencing cleanup reagents
BigDye XTerminator Purification Kit
The BigDye XTerminator™ Purification Kit is a fast, simple purification method for DNA sequencing reactions that removes unincorporated BigDye™ terminators and salts. No more dye blobs! Cleanup is complete in under 40 minutes and requires less than 10 minutes of labor.
- Liquid transfer eliminated with a single plate
- Complete dye blob removal Stabilized samples, primed for analysis
- Cleanup complete in under 40 minutes Less than ten minutes hands-on time End-to-end workflow solution
- Suitable for manual or automated protocols High quality data
Sanger Sequencing Kit
The Applied Biosystems Sanger Sequencing Kit provides a convenient and affordable solution for preparing sequencing reactions. The kit provides all the reagents needed for PCR cleanup, cycle sequencing, and sequencing product cleanup.
Convenient all-in-one package with optimal quantity for each component
More affordable Sanger sequencing sample preparation with built-in cost savings
Explore our comprehensive genotyping solutions and technologies
Genotyping microarrays
Microarray kits
Axiom™ Precision Medicine Diversity Array Kit, 96-format
PMD Research Array is a comprehensive imputation-aware array designed to drive insights into complex disease susceptibility, pharmacogenomics (PGx), and genetic factors underlying wellness and lifestyle in diverse populations to advance population genomics studies. It offers >850,000 markers selected for high genomic coverage from the 1000Genomes Project Phase III. Additional gene variants were chosen from broadly referenced public databases, including ClinVar, NHGRI-GWAS catalog, CPIC, PharmaGKB, and PharmaADME.
Axiom™ Precision Medicine Diversity Array Plus Kit, 96-format
PMD Research Array is a comprehensive imputation-aware array designed to drive insights into complex disease susceptibility, pharmacogenomics (PGx), and genetic factors underlying wellness and lifestyle in diverse populations to advance population genomics studies. It offers >850,000 markers selected for high genomic coverage from the 1000Genomes Project Phase III. Additional gene variants were chosen from broadly referenced public databases, including ClinVar, NHGRI-GWAS catalog, CPIC, PharmaGKB, and PharmaADME.
Axiom™ Asia Precision Medicine Research Array Kit, 96-format
PMRA is a comprehensive imputation-aware genotyping array with more than 750,000 markers. The high-value content on the array is associated with both common and rare diseases for clinical research and disease association studies in East and South Asian populations.
The Axiom™ PangenomiX Array brings the most ethnically diverse genomic coverage in a high throughput format array to researchers’ fingertips. This human genotyping research array was mindfully designed for whole genome imputation across global populations to enable scientists to create diverse genomic datasets that have traditionally been built on Caucasian cohorts.
The UK Biobank Axiom Array was designed by and for thought leaders in the human genetics community for high-throughput, high-value genotyping of large sample cohorts to explore the genetics of complex diseases and translational research with a single comprehensive low-cost solution.
Real-time PCR for genotyping
Real-time PCR reagents
TaqMan™ Genotyping Master Mix is optimized for end-point fluorescence detection in SNP genotyping applications.
- Distinct clusters and high call rates for unambiguous allelic discrimination
- Validated with TaqMan™ SNP Genotyping Assays
- Excellent pre- and post-PCR stability for high throughput setup and analysis
Real-time PCR Assays
Largest selection of predesigned assays for genotyping every SNP of interest as well as polymorphisms associated with drug metabolism (DME).
- Minor Groove Binder (MGB) technology enables TaqMan probes to discriminate between highly homologous allele sequences
- Robust design pipeline with 90+ parameters for optimal primer-probe combinations
- Functional quality testing with 20 gDNA samples for each human assay
- Gold-standard chemistry backed by more than 40,000 publications and over 300 patents
Sanger sequencing for genotyping
PCR cleanup reagents
ExoSAP-IT Express reagent: fastest PCR cleanup method
Accelerate result accuracy with confidence with ExoSAP-IT Express reagent. Our one-step solution that delivers higher yields and full PCR product recovery.
- Five-minute protocol—fast turnaround time
- One-tube, one-step PCR cleanup— add reagent directly to PCR product
- Novel enzyme technology— irreversibly inactivated in just one minute at 80°C
- Complete sample recovery—100% recovery of PCR products, regardless of amplicon length
- Eliminates spin columns or beads—helps to significantly decrease time and errors while increasing yield
Sequencing cleanup reagents
BigDye XTerminator Purification Kit
The BigDye XTerminator™ Purification Kit is a fast, simple purification method for DNA sequencing reactions that removes unincorporated BigDye™ terminators and salts. No more dye blobs! Cleanup is complete in under 40 minutes and requires less than 10 minutes of labor.
- Liquid transfer eliminated with a single plate
- Complete dye blob removal Stabilized samples, primed for analysis
- Cleanup complete in under 40 minutes Less than ten minutes hands-on time End-to-end workflow solution
- Suitable for manual or automated protocols High quality data
Sanger Sequencing Kit
The Applied Biosystems Sanger Sequencing Kit provides a convenient and affordable solution for preparing sequencing reactions. The kit provides all the reagents needed for PCR cleanup, cycle sequencing, and sequencing product cleanup.
Convenient all-in-one package with optimal quantity for each component
More affordable Sanger sequencing sample preparation with built-in cost savings
Simplify your DNA, RNA, and protein quantification with the Invitrogen™ Qubit™ 4Fluorometer and Qubit™ assays. Experience the simplicity of the Qubit 4 Fluorometer, with a touch screen and preprogrammed protocols specifically designed to couple with high-performing Qubit assays.
- Sensitive—extremely useful for diluted or low-abundance samples
- Specific—quantifies only the molecule of choice: DNA, RNA, or protein
- Accurate—quantify across a broad range with high reproducibility
- Tolerant—common contaminants are well-tolerated by assays
- Innovations
- Workflow
- Resources
- Cloning
- Plasmid Purification
- Verification
- Delivery
- Expression
KingFisher PlasmidPro Processor
Revolutionize your plasmid DNA extraction process for unparalleled efficiency and scalability with our cutting-edge innovation. The KingFisher PlasmidPro Maxi Processor introduces a breakthrough fully automated solution for maxi-scale purification.
- No centrifugation or setup needed; just pour culture and press start
- User-friendly protocols eliminate manual steps and errors
- Boosts productivity compared to manual workflows
MagMAX™ Pro HT NoSpin Plasmid Miniprep (coming soon)
Advanced Magnetic Bead Technology for Streamlined Plasmid DNA Purification
- Fastest plasmid miniprep extraction solution for transfection grade plasmid in 40 mins.
- Fully automated protocols utilizing KingFisher Presto and qualified liquid handler scripts
- Less plastic waste than traditional silica plate extraction methods
For over 20 years, Invitrogen™ GeneArt™ Gene Synthesis clones and GeneArt™ Strings™ DNA Fragments have been critical tools for research and discovery. Now you can experience the enhanced convenience of ordering multiple sequences with the newly updated Invitrogen™ GeneArt™ Services Dashboard.
Save time with these features:
- Optimize sequences in bulk with the world‑class Invitrogen™ GeneOptimizer™ algorithm
- Use personal templates to quickly apply your frequently used vector configurations and gene optimization settings
- Intuitively design the exact DNA you need, powered by the Invitrogen™ GeneArt™ Instant Designer™ tool
- Place and monitor your orders online
- Get an instant, no‑hassle price estimate
Featured resources for molecular cloning
Discover reagents and techniques across the molecular cloning workflow, with solutions ranging from restriction enzymes to gene synthesis, designed for speed and simplicity to help you obtain high-quality cloned DNA for your next discovery.
FastDigest Restriction Enzymes
FastDigest restriction enzymes are an advanced line of 176 restriction enzymes in a complete, one-buffer system for simplified cloning. Performs complete digestion in 5–15 minutes and is compatible with downstream applications.
Modifying Enzymes
Modifying enzymes offer reliable, high-quality, high-purity reagents, including DNA and RNA polymerases, phosphatases, kinases, and nucleases for an efficient cloning workflow.
DNA Vectors
A wide selection of vectors available in kits and as a stand-alone product to transfer foreign genetic material into a host cell for your downstream applications.
TOPO PCR Cloning
TOPO PCR kits enable cloning in 5 minutes with three easy steps, including Blunt-End, TA and Long Fragment cloning kits. Explore our selection tool to find the right kits for your cloning experiments.
Gateway Cloning Technology
Gateway recombination cloning can hellp empower gene shuttling, enabling you to access virtually any expression system, including E. coli, mammalian, and insect, in just a few simple steps.
Gibson Assembly® Cloning
Gibson Assembly® cloning kits are designed for efficient and seamless assembly of multiple DNA fragments of varying length into any vector. Excellent for multi-fragment assembly with GeneArt Strings DNA Fragments.
GeneArt Seamless Cloning
GeneArt Seamless Cloning kits enable the simultaneous and directional assembly of 1 to 4 PCR fragments, consisting of any sequence, into any linearized vector for diverse applications.
GeneArt Gene Synthesis Services
GeneArt Gene Synthesis is a reliable and cost-effective method for obtaining custom DNA fragments and constructs with 100% sequence accuracy. Optimize expression of synthetic genes, with our patented GeneOptimizer algorithm.
Thermo Scientific Cloning Tools
Thermo Scientific branded cloning tools, including PCR cloning kits, bacterial expression kits, cloning enzymes, competent cells, and DNA vectors for efficient cloning.
The choice of competent cells for transformation depends upon the transformation methods, strain genotypes, plasmid characteristics, and desired applications. Explore a wide range of chemically competent and electrocompetent E. coli cells for low- and high-throughput cloning.
Competent Cells Portfolio
Choose exceptional competent cells for your cloning applications and workflow from our wide range of single-use and high throughput, chemically competent and electrocompetent E. coli strains.
Chemically Competent Cells
Chemically competent cells are the most frequently used format in a wide array of applications and are transformed by heat shock.
Electrocompetent Cells
Electrocompetent cells are often preferred for library creation and low DNA input cloning applications due to very high transformation efficiencies achieved by electroporation.
Competent Cell Formats
Competent cells are available in versatile packaging formats, from single-use to high throughput, to fit your unique cloning workflow requirements.
Bacterial Growth Media
Choose from a wide selection of high quality ready-to-use or dehydrated E. coli bacterial growth media formats to suit your microbial culture needs.
Thermo Scientific Competent Cells
Thermo Scientific branded competent cells including, DH10B, DH5α, and BL21(DE3) for reliable and consistent performance for your cloning workflows.
Plasmid Purification Selection Tool
Molecular-grade plasmid purification
| Invitrogen PureLink Pro Quick 96 Plasmid Purification Kit | Invitrogen PureLink Quick Plasmid Miniprep Kit | Thermo Scientific GeneJET Plasmid Kits | |
|---|---|---|---|
 |  |  | |
| Prep size available | Small-scale 96-well plate format | Mini | Mini, Midi, Maxi |
| Purity grade/Endotoxin level | Molecular grade (>10 EU/µg) | Molecular grade (>10 EU/µg) | Molecular grade (>10 EU/µg) |
Transfection-grade plasmid DNA purification
| Invitrogen PureLink Fast Low-Endotoxin Purification Kits | Invitrogen PureLink HiPure Plasmid Kits | PureLink HiPure Plasmid Filter Kits | |
|---|---|---|---|
 |  |  | |
| Prep sizes available | Midi, Maxi | Mini, Midi, Maxi, Mega, Giga | Midi, Maxi |
| Purity grade/Endotoxin level | Transfection grade (0.1–1 EU/µg) | Transfection grade (0.1–1 EU/µg) | Transfection grade (0.1–1 EU/µg) |
Advanced transfection-grade plasmid DNA purification
| Thermo Scientific GeneJET Endo-Free Plasmid Maxiprep Kit | Invitrogen PureLink Expi Endotoxin-Free Plasmid Purification Kits | |
|---|---|---|
 |  | |
| Prep sizes available | Maxi | Maxi, Mega, Giga |
| Purity grade/Endotoxin level | Advanced transfection grade (<0.1 EU/µg) | Advanced transfection grade (<0.1 EU/µg) |
Automated plasmid purification
Applied Biosystems MagMAX Pro HT NoSpin Miniprep Plasmid Purification Kit | Thermo Scientific KingFisher Plasmid Pro | |
|---|---|---|
 |  | |
| Prep sizes available | Mini | Maxi |
| Purity grade/Endotoxin level | Transfection grade (0.1–1 EU/µg) | Transfection grade (0.1–1 EU/µg) |
Lipofectamine™ 3000 Transfection Reagent
Lipofectamine™ 3000 Transfection Reagent leverages our most advanced lipid nanoparticle technology to provide superior transfection performance with improved application outcomes and reproducible results. This reagent delivers superior transfection efficiency and improved cell viability for the widest range of hard-to-transfect and common cells (e.g., HEK293, HeLa).
Successfully transfect the widest variety of biologically relevant cell types with a reagent that offers:
- Superior performance—our highest efficiency reagent for difficult-to-transfect cells
- Improved cell viability—gentle on your cells, with low toxicity
- Versatile—one reagent for DNA, RNA, and co-transfection
Invitrogen™ Neon™ NxT Electroporation System
The Neon NxT Electroporation System is a next-generation electroporation platform leveraging the unique and trusted Neon electroporation technology, which increases transfection efficiency and cell viability. The system streamlines the transfection of mammalian cells, including primary, stem, and difficult-to-transfect cells, for fast and efficient delivery of nucleic acids.
Successfully transfect the widest variety of biologically relevant cell types with a reagent that offers:
- Proven transfection efficiency and cell viability — transfect challenging cell lines with high efficiency and high cell viability
- Flexibility when you need it — precisely optimize electroporation parameters for different cell types, cell densities, payloads, and electroporation protocols.
- Enhanced genome editing efficiency — optimized buffer for improved delivery of CRISPR molecules
- Save valuable research time — transfect cells quickly with an easy three step workflow, a single buffer kit and intuitive user interface
- Preserve your precious samples — minimize sample transfer loss and contamination risk
Trust the industry standard for rapid, high-yield transient HEK 293 expression
Every minute counts when it comes to expressing drug candidates. That's why the Gibco Expi293 Expression System is the tried-and-true choice for transient expression in mammalian 293 cells. This rapid, high-yield protein production system is based on high density culture of Expi293F(TM) Cells in Expi293(TM) Expression Medium and powered by Expifectamine(TM) 293, a cationic lipid-based transfection reagent. All of the system components work together to allow access to recombinant 293-derived proteins in just five to seven days, with a 2-10-fold increase in protein yields compared to previous generation transient expression systems. Get the protein yields that you need faster.
Maintain protein quality and function from research to bioproduction
Switching from 293 to CHO cells during drug development doesn't have to create uncertainty. Now there's a better way. The Gibco ExpiCHO Expression System is a completely optimized system consisting of ExpiCHO-S(TM) cells that have been adapted to high-density, serum-free suspension culture in ExpiCHO(TM) Expression Medium, along with specially designed transfection reagents and enhancers, that provide the highest yields possible in a transient system. That means you can start your research work in CHO cells and stay in CHO cells through discovery.
ExpiSf™ Expression System Starter Kits
The ExpiSf Expression System offers
- A chemically defined, yeastolate-free, animal origin-free medium for consistent cell growth and protein expression
- Integrated components that work in concert to deliver three times higher protein yields compared to traditional insect expression platforms
- Robust production of high-titer, high-quality P0 recombinant baculovirus in suspension culture format without the need for further virus amplification
- A fast, simplified workflow that allows you to obtain protein in as little as 6–10 days; half the time of traditional protocols
- Scalable expression that can be applied to cultures from 24-deep well plates to >3 L shake flasks
- Innovations
- Workflow
- Resources
- Reprogramming
- Cell Culture
- Engineering
- Differentiation
- Characterization
Gibco™ CultureCEPT™ Supplement (1000X)
A leap forward in cytoprotection: The Gibco™ CultureCEPT™ Supplement is a unique formulation that reduces cellular stress and improves viability of stem cells, differentiated cells, and primary cells during a wide variety of handling and processing steps where cell damage and death can limit the success of a cell culture workflow. It is a unique formulation that provides superior cell protection compared to traditional ROCK inhibitors, such as Y-27632, and other commercially available reagents.
- Provides exceptional cell protection compared to traditional ROCK inhibitors
- Achieve excellent cell survival in numerous applications including PSC passaging, embryoid body creation, organoid formation, single-cell cloning, gene-editing, differentiation, and recovery of cryopreserved cells
- Extensively tested by the NIH's NCATS (Chen et al., 2021)
- Compatible with trusted Gibco media systems
- Fully formulated and ready-to-use
Gibco™ CTS™ StemScale™ PSC Suspension Medium
CTS (Cell Therapy Systems) StemScale PSC Suspension Medium is a xeno-free medium that enables robust expansion of pluripotent stem cells (PSCs) in suspension for translational and clinical applications. CTS StemScale medium is scalable and easy-to-use, utilizing a spheroid self-assembly method to promote growth of multiple PSC lines without the use of microcarriers.
Features and benefits include:
- Maximal expansion capacity - achieve 5–10-fold expansion per passage and up to 30% higher expansion versus other PSC suspension media, reduce manufacturing time and cost
- Scalable expansion - promotes consistent growth of PSCs across multiple culture vessel formats and sizes (e.g., plates, flasks, and bioreactors) using the same culture parameters
- Utilizes a simplified workflow with no cell strainer
- GMP manufactured - facilitates regulatory filings with GMP manufacturing, traceability of raw materials, and regulatory documentation
Invitrogen™ Neon™ NxT Electroporation System
The Neon NxT Electroporation System is a next-generation electroporation platform leveraging the unique and trusted Neon electroporation technology, which increases transfection efficiency and cell viability. The system streamlines the transfection of mammalian cells, including primary, stem, and difficult-to-transfect cells, for fast and efficient delivery of nucleic acids.
- Proven transfection efficiency and cell viability — transfect challenging cell lines with high efficiency and high cell viability
- Flexibility when you need it — precisely optimize electroporation parameters for different cell types, cell densities, payloads, and electroporation protocols.
- Enhanced genome editing efficiency — optimized buffer for improved delivery of CRISPR molecules
- Save valuable research time — transfect cells quickly with an easy three step workflow, a single buffer kit and intuitive user interface
- Preserve your precious samples — minimize sample transfer loss and contamination risk
The EVOS M5000 Imaging System is a fully integrated digital inverted benchtop system for four-color fluorescence, transmitted light, and color imaging. The system now features EVOS Stage View, our proprietary functionality to track stage location through software.
- Sample location tracking intelligence
- Advanced color options
- Compatible with EVOS Onstage Incubator
Competent Cell Formats
Explore the modules to access free-to-use handbooks, virtual trainings, webinars, and the expertise you need for PSC research.
PSC Product Guide
Find key products and services to enable your PSC research using the PSC Product Guide
Stem Cell Research ‘How to’ Videos
Our “How-to” video provides a step-by-step training for a variety of stem cell techniques, from reprogramming to characterization.
Make the switch to Nunc products
The Switch-to-Nunc cross reference tool can be used to find direct replacements for Corning™, Costar™, Falcon™, Eppendorf™, Sarstedt™, and Greiner™ cell culture plastics. Nunc offers cell-type optimized surfaces, as well as innovative cell culture plates, flasks, and inserts.
Gibco PeproTech recombinant proteins for stem cell differentiation
Identify specific recombinant proteins used to differentiate PSC into your cell of interest.
Gibco Peprotech recombinant proteins for organoid and tumoroid models
Downloadable list of Gibco PeproTech recombinant proteins by organoid type.
Gibco Neurobiology Protocol Handbook
Find neurobiology protocols, solutions, and resources put together by experienced scientists in this handbook.
Gene editing learning center
Explore free downloads, online tutorials, and scientific webinars to maximize your success with CRISPR and TALEN applications!
Invitrogen TrueDesign Genome Editor
The TrueDesign tool supports a variety of edits—gene knockout, gene tagging, insertion, replacement, deletion, and SNP creation. Use this free online tool to easily design, select, and order reagents for your gene editing experiments!
Reprogramming Kit
CytoTune™-iPS 2.0 Sendai Reprogramming Kit
- Ease of use—requires only one application of virus for successful reprogramming
- Efficient—up to 2% reprogramming efficiency
- Rapid clearance—the non-integrating Sendai virus remains in the cytoplasm, reducing the likelihood of integration into the host genome
- Zero footprint—the vectors and transgenes can be eliminated from the cells
CTS CytoTune-iPS 2.1 Sendai Reprogramming Kit
- This product is manufactured at a site with a process that was audited for conformity with current Good Manufacturing Practices for medical devices, 21 CFR Part 820 of the regulation.
- The bovine serum albumin carrier protein was removed from the CTS CytoTune-iPS 2.1 Sendai vector formulation. The preparation contains no animal-derived components at the primary component level.
- Full traceability documentation such as Certificate of Origin and Certificate of Analysis are available.
Feeder-free PSC culture media
StemScale PSC Suspension Medium |
StemFlex Medium |
Essential 8 Medium |
Essential 8 Flex Medium | |
| Ideal for: | Robust expansion of PSCs in suspension | Robust maintenance of difficult cell lines or performing single-cell passaging and gene editing | Routine and consistent PSC expansion | Routine PSC culture with flexible feeding schedule |
| Culture format | Suspension (PSC spheroids) | Adherent | Adherent | Adherent |
| Formulation | Contains animal-origin components | Contains animal-origin components | Xeno-free | Xeno-free |
| Recommended matrix | N.A. | Geltrex matrix rhLaminin-521 | Vitronectin (VTN-N) rhLaminin-521 | Vitronectin (VTN-N) rhLaminin-521 |
| Manufactured in cGMP-compliant facility | Yes | Yes | Yes | Yes |
| Flexible feeding schedule (skip feeding days; weekend-free) | Yes | Yes | No | Yes |
| Scalability | Best | Good | Good | Good |
| Supports gene editing | N.A. | Best | Good | Good |
| Gibco™ CTS™ version ? | A5869601new! | In development | A2656101 | Not currently |
Supplements and reagents
- Provides exceptional cell protection compared to traditional ROCK inhibitors
- Achieve excellent cell survival in numerous applications including PSC passaging, embryoid body creation, organoid formation, single-cell cloning, gene-editing, differentiation, and recovery of cryopreserved cells
- Extensively tested by the NIH's NCATS (Chen et al., 2021)
- Compatible with trusted Gibco media systems
- Fully formulated and ready-to-use
- Contains a xeno-free cryopreservation medium, PSC Cryomedium, and a chemically defined recovery supplement, RevitaCell™ Supplement
- Reliable, easy-to-use and versatile
- Achieves 50% more viable cells than traditional methods of PSC cryopreservation
- Xeno-free, chemically defined media maintains normal morphology, pluripotency, and karyotype of pluripotent stem cells over 10 passages
Nunc Cell Culture Plastics and Cultureware
Thermo Scientific Nunc cell culture plastics offer a solution for every step of your workflow. Whether you need cell culture-treated plates for imaging or low-attachment dishes for your 3D work, easily find your cell cultureware by searching by surface, format, or other application needs.
Nunclon Supra surface for advance cell culturing
Compatible with 40+ cell lines, Thermo Scientific Nunc dishes, flasks and plates with Nunclon Supra surface show improved yield and morphology for primary cells and MSCs while enabling xeno-free or low-serum research applications.
Try the Nunclon Supra surface today: Dishes | Plates | Flasks | 96-well plates
Find out more about the Nunclon Supra surface today at thermofisher.com/supra
CRISPR design tool
The Invitrogen TrueDesign Genome Editor is a free online tool that enables scientists of all experience levels to easily design, select, and order reagents for accurate and successful gene editing experiments.
- Supports six types of edits—gene knockout, fluorescent tagging, insertion, deletion, SNP creation, and replacement
- Easily extract the transcript and loci you want to modify across model species
- View the DNA and amino acid sequences while editing your gene with point-and-type features
- Generate your specific donor and the associated CRISPR-Cas9 gRNA or TALEN sequences
CRISPR gRNA
- Choose from our catalog of ready-to-transfect predesigned sgRNAs for efficient knockout.
- Upload your custom sequence to our Invitrogen TrueGuide gRNA Ordering Tool.
- Design your custom edit with our TrueDesign Genome Editor software.
CRISPR protein
- Near complete elimination of off-target events in all tested cell lines including standard, immune, primary, and stem
- Maintains maximum on-target editing efficiency compared to TrueCut Cas9 Protein v2
- High quality—manufactured under strict ISO 13485 quality standards
- Transfection-ready, using either electroporation or lipid-mediated transfection reagents
- Eliminates time-consuming cloning steps
Knock-in Editing and Gene Tagging
- Achieve up to 100% edited cells—get great results, regardless of your level of expertise
- Eliminate cloning steps—one-step PCR gives you donors in hours, not days
- Minimize screening time—enrich edited cells using blasticidin or puromycin
- Tag genes easily—add your choice of a fluorescent or epitope tag to a protein of interest
- Create knockout cell lines—combine functional knockout with fluorescence and selection markers to enrich edited cells
Transfection
Neon NxT Electroporation System |
CTS Xenon Electroporation System |
Lipofectamine Stem |
Lipofectamine CRISPRMAX | |
DNA |  | | | |
mRNA | | | | |
RNAi | | | | |
CRISPR-Cas9 | | | | |
Antibody / proteins | | | ||
Cell types | Over 140 cell lines tested; excels in hard-to-transfect cells, primary cells, and stem cells | Suitable for CAR T cell knock-in and knockout. High transfection efficiency, even with hard-to-transfect cells such as primary, stem, and immune cells. | Suitable for stem cells, except HSCs | Tested in over 20 cells types including iPSC, mESC, N2A, CHO, A549, HCT116, HeLa, HEK293, and several others |
Interested to find a great transfection reagent for your cell type and payload ?
Differentiation kits
Gibco™ PSC Cardiomyocyte Differentiation Kit
Gibco™ PSC Dopaminergic Neuron Differentiation Kit
Gibco™ PSC Definitive Endoderm Induction Kit
Gibco™ PSC Neural Induction Medium Kit
- Ready to use kits—differentiate PSCs into commonly used cell types
- Rapid—generate high-quality functional cells in fewer steps than traditional methods found in the literature
- Reproducible—flexible set of tools provides more control and reliable results
Growth factors
Gibco™ PeproTech™ recombinant protein
- More than 700 recombinant proteins from research to commercial grade in multiple vial sizes
- In-house manufacturing for scale, reliability, and consistency; complete documentation, stringency, and quality assurance for clinical trials
- Dedicated customer service and technical support teams to enable timely responses to your needs
Recommended products for your desired cell lineage
Ectoderm is the outer layer of the early embryo and gives rise most notably to neural cells and skin.
Recommended products:
Mesoderm is the middle layer of the developing embryo and gives rise to muscle, kidney, and blood.
Recommended products:
- PSC Cardiomyocyte Differentiation Kit
- Human Cardiomyocyte Immunocytochemistry Kit
- Geltrex LDEV-Free hESC-qualified Reduced Growth Factor Basement Membrane Matrix
- Fluo-4 NW Calcium Assay Kit
- StemPro Osteogenesis Differentiation Kit
- StemPro Adipogenesis Differentiation Kit
- StemPro Chondrogenesis Differentiation Kit
- Essential 6 Medium
Endoderm is the innermost primary germ layer and gives rise to multiple systems including gut, liver, lung, pancreas, and thyroid.
Recommended products:
Characterization tools
Quantitative analysis of trilineage differentiation potential
The assay offers:
- A quantitative and time-saving alternative to teratoma formation [3]
- Comparison of expression profiles to a reference standard
- An easy-to-use platform with pre-plated assays and dedicated, intuitive analysis software
PrimeView Global Gene Expression Profile Assays
Confirm pluripotency
The assays offer:
- Compatibility with the PluriTest Online Analysis Tool, a published and established method with over 16,000 samples analyzed
- More than 36,000 transcripts and variants are compared against an extensive reference set of more than 450 samples
- A free and simple-to-use cloud-based analysis tool
Verify genomic stability
The assays offer:
- Accurate detection of chromosomal abnormalities
- Karyotyping and genotyping (sample ID) with a single assay
- Simple analysis tool that does not require cytogenetic expertise
- Results in 3–4 days
Cell analysis instrumentation
Quickly count cells, measure cell viability, and monitor fluorescent protein expression. Insert the slide, and the Countess 3 FL will auto-illuminate, focus, capture, and count.
Designed to eliminate the complexities of microscopy without compromising performance, our systems make cell imaging accessible to almost every lab and budget.
Equipped with a flexible range of measurement technologies including Absorbance, Fluorescence Intensity, Luminescence, AlphaScreen, and Time-Resolved Fluorescence.
For maximal productivity and efficiency, use our high-content instruments with our extensive selection of reagent- and cell line-based assays.
- Innovations
- Workflow
- Resources
- Sample Prep
- Immunophenotype
- Buffer Selection
- Cell Health Dyes
- Experimental Controls
Attune™ CytPix™ Flow Cytometer
The Attune CytPix Flow Cytometer combines bright-field images with high sensitivity and precision flow cytometry in a small footprint and is simple to use at any experience level. Automated image analysis with the Attune Cytometric Software translates event features into distinct morphometric parameters that can be combined with standard fluorescence and scatter parameters.
- Acoustic focusing for sensitive analysis
- Fast sample acquisition speed
- Clog resistant fluidics
The Invitrogen Bigfoot Spectral Cell Sorter is a fluorescence-activated cell sorter designed with cell sorting technology that can expand your reach while fitting your workflow. With up to 9 lasers and 60 detectors, you can run your existing experiments using conventional compensation, or take advantage of the spectral cell sorting capabilities to resolve even more cell populations.
- Exceptionally fast high-throughput cell sorting
- Integrated biocontainment and aerosol management
- Conventional compensation or spectral unmixing
Dynabeads™ MyOne™ CD45 Leukocyte Depletion
Dynabeads MyOne CD45 Leukocyte Depletion is a highly efficient product designed for depletion of human CD45-positive cells from whole blood, buffy coat, or peripheral blood mononuclear cells (PBMC). After discarding the bead-bound cells, the product allows for utilization of the remaining untouched rare target cells, such as circulating tumor cells (CTCs), in downstream applications.
- Efficient depletion of human leukocytes from PBMC and whole blood
- Enrichment of viable and untouched CTCs by leukocyte depletion
- High depletion efficiency of contaminating granulocytes in whole blood samples
FREE—Flow Cytometry Panel Design Service and Panel Builder Tool
Our scientists can design panels for you as many times you need—free of charge, no product purchase needed.
Request Free Panel Design service
Fluorescence SpectraViewer
Assess the spectral compatibility of dyes and probes for experiments that utilize fluorescence detection techniques.
Learn more
Flow Cytometry Protocols Handbook
The Flow Cytometry Protocols Handbook will help you accelerate your research to help you plan and set up your flow cytometry experiments.
Download now
Fluorophore and Reagent Selection Guide for Flow Cytometry
A handy reference poster featuring the broad range of Invitrogen labeling reagents available for flow cytometry
Request Free Panel Design service
Dynabeads Products Selection Guide
Dynabeads magnetic beads are widely used for various applications. To ensure successful results, it is important to choose the appropriate Dynabeads magnetic beads for your research. This selection guide is designed to help you select the right beads based on you research needs.
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Cell Isolation and Cell Separation
Cell isolation can be achieved using magnetic beads to target cells for selection or depletion by using ligands or antibodies directed against specific cell surface antigens. Labeled cells that are cross-linked to magnetic beads are immobilized using magnets or magnetic racks.
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Step 1: Sample preparation
eBioscience™ 10X RBC Lysis Buffer (Multi-species)
Catalog number: 00-4300-54
Contains ammonium chloride, which lyses red blood cells with a minimal effect on lymphocytes when used as instructed. This buffer can be used for lysis of erythrocytes in single-cell suspensions of peripheral blood and hematopoietic tissues from human, mouse, rat, canine, and non-human primate.
Accutase - Enzyme Cell Detachment Medium
Catalog number: 00-4555-56
Can detach cells for flow cytometry analysis in a mild way. Accutase has been shown effective on: fibroblasts, keratinocytes, vascular endothelial cells, hepatocytes, vascular smooth muscle cells, hepatocyte progenitors, etc.
Cell Isolation Using Biotinylated Antibodies
- Any cell type — Use any biotinylated antibody
- Any species — Human, mouse, rat, non-human primates
- Any sample — Whole blood, bone marrow, tissue digests, spleen
- Any application — Cells are ideal for culture, stimulation, DNA/ RNA or protein purification, flow cytometry
Step 1a: Cell isolation and depletion
Negative cell isolation
Negative cell involves labeling unwanted cell types for removal. The unwanted cell types can be bound using magnetic beads coupled to antibodies or ligands that target specific cell surface proteins. The bound, unwanted cells can be depleted from the final isolated fraction by incubating the sample in a magnetic field, leaving desired unbound cells free floating in solution.
Negative cell isolation with Dynabeads magnetic beads
In negative cell isolation, Dynabeads magnetic beads are used to deplete all the unwanted cells using the supplied antibody mix. The target cells are left in the sample untouched and free of beads. The isolated target cells can be directly analyzed in a flow cytometer and used in any functional assay.
Negative cell separation using magnetic beads:
- Is a gentle, tube-based method
- Isolates high yields of pure, viable, and functional cells
- Preserves the cells’ native characteristics
Human negative cell isolation kits
 | Negative cell isolation kits are available for human cells to target the following cell types: |
| Cell type targeted | Cell surface marker(s) to target for isolation | Format available |
|---|---|---|
| T helper cells, regulatory T cells, and natural killer T cells | CD4 | Untouched kit |
| Cytotoxic T cells | CD8 | Untouched kit |
| Dendritic cells (DCs) | CD3, CD14, CD16, CD19, CD56, and CD235a | Untouched kit |
| Monocytes | CD3, CD7, CD16 (a and b), CD19, CD56, CD123, and CD235a | Untouched kit |
| Pan B cells | CD2, CD14, CD16 (a and b), CD36, CD43, and CD235a | Untouched kit |
| Pan T cells | CD14, CD16 (a and b), CD19, CD36, CD56, CD123, and CD235a | Untouched kit |
| Natural killer (NK) cells | CD3, CD14, CD36, HLA Class II, CD123, and CD235a | Untouched kit |
Mouse negative cell isolation kits
 | Negative cell isolation kits are available for mouse cells to target the following cell types: |
| Cell type targeted | Cell surface marker(s) to target for isolation | Format available |
|---|---|---|
| Naive CD4+ T cells | CD4 | Untouched kit |
| Naive CD8+ T cells | CD8 | Untouched kit |
| Dendritic cells (DCs) | CD2, CD3ε, CD49b, mIgM, and Ter-119 | Dynabeads kit |
| Pan B cells | CD43 | Dynabeads kit |
| Pan T cells | CD11b, CD16/32, CD45R, and Ter-119 | Untouched kit |
Step 1b: Build-your-own positive cell isolation kit
| Use with | Format available |
|---|---|
| Any antibody targeting any cell type in any species | FlowComp kit |
| Any biotinylated antibody targeting any cell type in any species | CELLection kit |
Step 2: Immunophenotyping with antibodies
NovaFluor™ dyes for immunophenotyping
NovaFluor™ dyes are designed for more resolution with narrow emission spectra and minimal cross-laser excitation. Lower spectral spillover or overlap lessens the need for compensation, decreases spreading error, and increases opportunities to add new markers. This aids in construction of flow cytometry panels with increased resolution while expanding the overall size of panels.
- Unique spectral signatures
- Designed with narrow emissions and minimal cross-laser excitation
- Decreased spillover spread for higher resolution
- Stable dyes that can be stored long-term without losing fluorescence as compared to tandem dyes
- Easy to add to existing panels
Immunophenotyping with flow cytometry antibodies
A multicolor flow cytometry panel uses two or more primary conjugated antibodies to identify single cells by detecting multiple antigens. The goal of the panel is to get the maximum signal for effective visualization of cell populations.
- Efficiently build a simple or complex panel with Flow Cytometry Panel Builder online tool.
- Our free Panel Design Service connects you to a team of scientists that will provide you with specialized expert support when building or optimizing your multicolor panel.
- Expand your panel with our wide range of fluorophores for both traditional and spectral analysis.
Brilliant Ultra Violet™ (BUV) conjugated antibodies
BUV dyes use polymer technology to absorb energy and emit light signal. By incorporating BUV dyes in their experiments, scientists can take advantage of a broader range of instrument lasers and channels—including UV—to help reduce compensation necessary in panels with multiple conjugated antibodies. Super Bright dyes and Brilliant Violet™ dyes use similar polymer technologies.
Step 3: Buffer selection
Flow cytometry buffers for sample preparation
Sample preparation is an important step for obtaining statistical value from flow cytometry experiments. Sample preparation reagents for flow cytometry include cell surface staining, intracellular and transcription factor staining buffer sets, cell lysis assays, blocking reagents and magnetic cell isolation beads. Benefits of using these buffers include the following:
- Retains inherent biological characteristics
- Reduce background staining
- Quicker and easier preparation with optimized kits
| Cytoplasmic staining | Nuclear staining | ||
|---|---|---|---|
| eBioscience™ Intracellular Fixation & Permeabilization Buffer Set | FIX & PERM™ Cell Fixation & Cell Permeabilization Kit | eBioscience™ Foxp3 / Transcription Factor Staining Buffer Set | |
| Use |
|
|
|
| Contents |
|
|
|
| Size | 1 kit | 50 tests | 1 kit |
| Cat. No. | 88-8824-00 | GAS003 | 00-5523-00 |
| Regulatory status | RUO–For Research Use Only. Not for Use in Diagnostic Procedures. | GPR–General Purpose Reagent. | |
| When to use | For use in research only (i.e. research on any sample type). | For use in general laboratory application, that is used to collect, prepare, and examine specimens from the human body for diagnostic purposes. | |
Step 4: Cell Health dyes
Save time and avoid inaccurate analysis of your cell population by removing dead cells and unwanted artifacts efficiently with the Invitrogen LIVE/DEAD Fixable Dead Cell Stains. The Invitrogen LIVE/DEAD Fixable Viability Dyes are fixable viability dyes that help to ensure accurate assessment of cell viability in samples after fixation and/or permeabilization.
- Flexible - 14 different LIVE/DEAD dyes to choose
- Robust - clear distinction of live and dead cells is preserved for up to 30 days after fixation
- Simple - fit into almost any staining and immunophenotyping protocol
- Unique - 14 novel spectral signatures
Step 5: Experimental Controls
Staining intracellular targets for flow cytometry requires the use of multiple antibodies that often have overlapping fluorescence emission spectra. The emission spillover must be compensated for correctly in order to ensure that the fluorescent signal being measured is due to the fluorochrome of interest and thus the cell population of interest.
UltraComp eBeads™ Plus Compensation Beads
Catalog number: 01-3333-42
Advantages of UltraComp eBeads Plus microspheres:
- Optimized for the violet laser with added compensation resolution for Super Bright Polymer Dyes and Brilliant Violet Dyes
- Supplied in a single vial—one drop contains both antibody-coated positive and uncoated negative beads
- Suitable for multiple species: human, rabbit, hamster, mouse, and rat
- used in compensation with all fluorochromes excited by ultraviolet (355 nm) violet (405 nm), blue (488 nm), green (532 nm), yellow-green (561 nm), and red (633-640 nm) lasers
- Recognizes both kappa and lambda chains
- Innovations
- Workflow
- Resources
- Cell Culture
- Cell Preparation
- Label
- Detect
- Protect
CellInsight™ CX7 LZR Pro High Content Screening Platform
The Thermo Scientific CellInsight CX7 LZR Pro High Content Screening (HCS) Platform is a fast, laser light engine-based, automated cellular imaging and analysis platform designed for life scientists who require on-the-fly quantitative analysis.
- Back-illuminated sCMOS camera offers a 95% peak quantum efficiency and -20°C cooling
- 7-channel fluorescent imaging with laser illumination
- EurekaScan Finder double-pass functionality for on-the-fly seek and find of spheroids, tissue, and rare cellular events
The EVOS M5000 Imaging System is a fully integrated digital inverted benchtop system for four-color fluorescence, transmitted light, and color imaging. The system now features EVOS Stage View, our proprietary functionality to track stage location through software.
- Sample location tracking intelligence
- Advanced color options
- Compatible with EVOS Onstage Incubator
Cellular Imaging Resource
Find imaging reagents, buffers, instruments, software, and microscopes for your immunofluorescence and immunohistochemistry experiments
Explore now
Invitrogen™ Stain-iT™ Cell Staining Simulator
Use our free, web-based tool to visualize the expected reagent staining pattern on a virtual cell.
Try now
Imaging protocol handbook
Protocols that fit your needs in imaging ranging from sample and assay preparation to staining, labeling, and data analysis strategies.
Download now
Microscopy Protocols
Find step-by-step instructions for your imaging experiments.
Learn more
Nunc cell culture imaging product selection guide
The Thermo Scientific Nunc Cell Culture Imaging Products offer comprehensive solutions with flexibility and consistency to meet your needs in cell imaging applications.
Download now
Cell Culture Workflow Solutions
Culture with confidence
Learn more about how our media, FBS, and plastics are validated for exceptional product performance so that you can culture with confidence.
Learn more
Cell Culture Select Tool
Easily search by cell line for the products you need.
Try the tool
Secondary Antibodies
We offer a comprehensive portfolio of conjugated and unconjugated secondary antibodies for fluorescent and chemiluminescent detection of validated* primary antibodies in a wide range of applications, such as cell imaging, flow cytometry, and western blotting.
Learn more
Cell Culture Imaging Dishes, Plates, Slides and Coverglass
The Thermo Scientific Nunc Cell Culture Imaging Products offer comprehensive solutions with flexibility and consistency to meet your needs in cell imaging applications, including fluorescence microscopy, phase contrast microscopy, confocal microscopy, live cell imaging, differential interference contrast (DIC) microscopy, and fluorescence in situ hybridization (FISH).
Featuring removable medium chambers, the Thermo Scientific Nunc Lab-Tek and Lab-Tek II Chamber Slide systems allow you to seed, incubate, fix, stain and image on a single microscope slide, saving time and labor in the immunohistochemistry work.
Innovative cell culture media formulation
We are committed to innovating Gibco cell culture media. We go beyond basic formulations to provide optimized and innovative cell culture solutions in support of your efforts to achieve consistently reproducible results.
BenchStable Media
Gibco BenchStable Media, available in our most common cell culture media formulations, are stable at room temperature, allowing for convenient and flexible storage without utilizing cold storage space.
Explore Gibco BenchStable Media
Human Plasma-Like Medium
Gibco Human Plasma-Like Medium (HPLM) is formulated to mimic the metabolic profile of human plasma, allowing for improved physiological relevance when studying cancer and disease.
Explore Gibco Human Plasma-Like Medium
Media with GlutaMAX Supplement
Media with Gibco GlutaMAX Supplement are standard cell culture formulations containing a stabilized form of L-glutamine that prevents degradation and buildup of ammonia.
Explore GlutaMAX Media formulations
Prepare
ReadyProbes™ Hydrophobic Barrier Pap Pen
The ReadyProbes Hydrophobic Barrier Pap Pen creates a water-repellent barrier when used on a microscope slide or coverslip. By lightly drawing a rectangle or circle around the tissue section or cells on the microscope slide, aqueous incubation reagents can be kept inside the circle or rectangle. Save precious reagents by incubating with less volume.
- Save precious reagents
- Keep reagents separate
Fixation/Permeabilize/ Block
Image-iT™ Fixation/Permeabilization Kit
The Image-iT Fixation/Permeabilization Kit is a convenient-to-use fixation/permeabilization kit that contains all the necessary reagents to prepare cells for antibody staining and imaging.
Triton™ X-100 Surfact-Amps™ Detergent Solution
Thermo Scientific Triton X-100 Surfact-Amps Detergent Solution is a highly-purified Triton™ X-100 detergent stabilized as a 10% solution (w/v).
BlockAid™ Blocking Solution
BlockAid™ Blocking Solution is superior to commonly used blocking reagents such as bovine serum albumin, normal serum, or casein for use with cells or tissue sections.
The Image-iT Cell Painting Kit conveniently provides six different fluorescent labels to assess cellular morphology by high content screening for drug discovery. Cell painting is useful for identifying cellular phenotypic changes, mechanisms of action, toxicity testing, and cellular profiling during high-content drug screening.
- An optimized format for high-content screening
CellEvent™ Caspase-3/7 Detection Reagents
CellEvent™ Caspase-3/7 Detection Reagents is easy to use reagent for apoptosis analysis. It is compatible with both live cell fluorescence-imaging and formaldehyde-based fixation methods
- Optimized caspase-3/7 substrate for apoptosis analysis
- Easy to use—add and read; no cell lysis require
- Use for time course measurements; easily select the time point of interest
Image-iT™ Red Hypoxia Reagent is a novel fluorogenic compound for measuring hypoxia in live cells. It is non-fluorescent when live cells are in an environment with normal oxygen concentrations and becomes fluorescent when oxygen levels are decreased.
- Measures hypoxia in live cells by fluorescing in low oxygen environments
- Real-time oxygen detector, with reversible fluoregenic response
- Easy to use—just add to cell culture media and image
Alexa Fluor™ 488 Tyramide SuperBoost™ Kit, streptavidin
SuperBoost kits combine the brightness of AlexaFluor™ dyes with the superior signal amplification of a poly-HRP-mediated tyramide labeling reaction to produce a sensitivity 10-200 times greater than standard methods.
- Superior sensitivity for detection of low-level or hard-to-detect targets by fluorescent imaging
- Simple protocol and detection using standard filters
- Suitable for high-resolution multiplex images—co-label with DAPI, secondary antibodies, and other SuperBoost kits
- Requires 10-100 times less primary antibody then standard ICC/IHC/ISH experiments
SuperBoost™ EverRed and EverBlue Colorimetric HRP Kits
The kits are simple to use and easily adapted to standard IHC experimental protocols using any tissue type, including formalin-fixed, paraffin-embedded (FFPE) and cryopreserved tissue. Tissues labeled using the SuperBoost EverRed and EverBlue kits can be imaged using any type of brightfield microscope, producing high-resolution images.
- Permanent colorimetric staining that is also fluorescent, allowing confirmation of the staining pattern with both brightfield and fluorescence imaging
- Compatibility with other common colorimetric counterstains
- Multiplexability between EverRed, EverBlue, and DAB
Cell Imaging Instruments
CellInsight™ CX7 LZR Pro High Content Screening Platform
The Thermo Scientific CellInsight CX7 LZR Pro High Content Screening (HCS) Platform is a fast, laser light engine-based, automated cellular imaging and analysis platform.
EVOS™ M5000 Imaging System
The EVOS M5000 Imaging System is a fully integrated digital inverted benchtop system for four-color fluorescence, transmitted light, and color imaging.
EVOS™ M3000 Imaging System
The EVOS M3000 Imaging System is a fully integrated digital inverted benchtop microscope for two-color fluorescence, transmitted light, phase contrast, and color imaging. It is excellent for routine cell/tissue culture imaging applications.
ProLong™ Gold Antifade Mountant
ProLong Gold Antifade is specifically designed to help protect fluorescent dyes (i.e. Alexa Fluor) from fading but is not recommended with fluorescent proteins (GFP, RFP, mCherry). It comes ready-to-use and stored at room temperature—just apply a drop on the sample, add a coverslip, cure, and image.
- Protect fluorescent dyes from fading (photobleaching)
- Refractive index (RI) of 1.47
SlowFade™ Diamond Antifade Mountant
SlowFade Diamond Antifade Mountant is a glycerol-based liquid mountant applied directly to fluorescently labeled cells or tissue samples on microscope slides. It provides superior antifade protection across the entire visual and IR spectrums. You can use SlowFade Diamond mountant with almost any fluorescent dye or fluorescent protein (e.g., GFP, RFP, mCherry) to achieve the brightest signal and lowest background.
- Protects fluorescent dyes and fluorescent protein from fading, across entire visible and IR spectrums
- Ready-to-use liquid that does not cure; samples can be viewed immediately
CytoVista™ 3D Cell Culture Clearing Reagent
CytoVista 3D Cell Culture Clearing Reagent clears fluorescently labeled 3D cultured cells such as organoids and spheroids. This clearing process enables sharp and bright 3D fluorescent imaging for depths up to 1000 μm. The reagent can also be used as an imaging solution with stable storage of 3D cell cultures for weeks with minimal change in tissue morphology or brightness, or specificity of the fluorophores.
- Rapid clearing of fluorescently labeled tissue for 3D imaging
- Minimal shrinkage, expansion, or other morphological changes to tissue
- Compatible with most fluorophores, including dyes and fluorescent proteins
- PCR
- Extraction
- DNA Extraction
- Lab Essentials
- RNA Extraction
- PCR Cleanup
- Nuclease Control
- Magnets
- Enzyme Selection
- DNA Polymerases
- Thermal Cycling
- PCR Plastics
- Analysis
- Agarose
- DNA Stains
- DNA Ladders
- Reagents/Buffers
- Gene & Genome Profiling
- Gene Expression
- qPCR
- Microarrays
- Mutation Detection
- CE Sequencing
- SNP Genotyping
- qPCR
- CE Sequencing
- Arrays
- Cloning & Protein Expression
- Cloning
- Cloning Enzymes and Kits
- Plasmid Purification
- Cloning Grade
- Transfection Grade
- Buffer Kits & Accessories
- Verification
- Agarose
- DNA Stains
- DNA Ladders
- Reagents/Buffers
- Delivery
- Transfection Reagents
- Induced Pluripotent Stem Cells
- Reprogramming
- Reprogramming Kits
- Cell Culture
- Matrices
- Feeder Cell Layer
- Culture Media
- Supplement & Reagents
- Dissociation/Passaging
- Cell Culture Plastics
- Cryopreservation
- Engineering
- Engineering
- Differentiation
- Differentiation
- Characterization
- Characterization
- Flow Cytometry
- Sample Prep
- Cell Enrichment & Depletion
- Pre-staining Cell Prep
- Fixation and Permeabilisation
- Immunophenotyping
- Immunophenotyping
- Cell Health Dyes
- Cell Health
- Experimental Controls
- Controls
- Cell Imaging
- Cell Culture
- Cell Culture Plastics
- Cell Culture Media
- Cell Culture Reagents and Supplements
- Cell Preparation
- Fix, Permeabilize, Block
- Label
- Label
- Detect
- Detect
- Protect & enhance
- Protect & enhance
Promotions
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