Invitrogen™
Dynabeads™ Human DC Enrichment Kit
Use this kit to enrich viable human dendritic cells (DC's) from PBMC by negative isolation. The kit depletes T cells, Read more
Catalog number 11308D
Price (USD)/ 2 x 10 mL
1,166.00
-
Price (USD)/ 2 x 10 mL
1,166.00
Dynabeads™ Human DC Enrichment Kit
Catalog number11308D
Price (USD)/ 2 x 10 mL
1,166.00
-
Use this kit to enrich viable human dendritic cells (DC's) from PBMC by negative isolation. The kit depletes T cells, B cells, monocytes/ macrophages, granulocytes, NK cells and erythrocytes. Highly enriched DC's are bead and antibody-free, and can be used in any functional assay. The cells should be further sorted to obtain high purity of the specific DC sub-populations of choice.
• Obtain high recovery (80-100%) of viable DC's of all sup-populations.
• Substantial reduction in sorting time and volume compared to sorting only, thus saving money as well.
Methodology:
An antibody mix towards the non- dendritic cells is added to the sample and allowed to bind to the cells. Dynabeads™ are added and will bind to the antibody-labeled cells during a short incubation. The bead-bound cells are quickly separated on a magnet and discarded. The remaining untouched human DC's can be directly sorted into the sup-population of choice or used in any other application.
Starting samples:
PBMC
• Obtain high recovery (80-100%) of viable DC's of all sup-populations.
• Substantial reduction in sorting time and volume compared to sorting only, thus saving money as well.
Methodology:
An antibody mix towards the non- dendritic cells is added to the sample and allowed to bind to the cells. Dynabeads™ are added and will bind to the antibody-labeled cells during a short incubation. The bead-bound cells are quickly separated on a magnet and discarded. The remaining untouched human DC's can be directly sorted into the sup-population of choice or used in any other application.
Starting samples:
PBMC
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell TypeDendritic Cells
Product TypeCell Enrichment Kit
Isolation TechnologyNegative Isolation
No. of CellsProcesses ∼2 x 109 cells total
Output Viability>95%
Product LineDYNAL™, Dynabeads™
Purity or Quality GradeResearch Grade
Quantity2 x 10 mL
Sample TypePBMC
Shipping ConditionRoom Temperature
Starting Material Cell No.1 x 107 PBMCs per isolation
Target SpeciesHuman
Unit Size2 x 10 mL
Contents & Storage
The Kit Contains: 2 x 10 mL Depletion MyOne™ Streptavidin Dynabeads™ and 4 mL Antibody Mix containing an optimized mixture of biotinylated monoclonal antibodies towards CD3, CD14, CD16, CD19, CD56 and Glycophorin A.
Store at 2°C to 8°C.
Store at 2°C to 8°C.