Figure 1. RNA quality on the Agilent Bioanalyzer. Total RNA, including small RNA, was isolated from 6 different tissue types (A: Mouse Brain; B: Mouse Heart; C: Mouse Liver; D: Mouse Spleen; E: HeLa cells; and F: Jurkat cells) using two different isolations methods and kits. Results shown in blue are for RNA isolated using the mirVana miRNA Isolation Kit and results shown in red are for RNA isolated using the MagMAX-96 mirVana Total RNA Isolation Kit that provides equivalent or improved results.
Total RNA Isolation and microRNA from a Variety of Samples
- Process 6 to 96 samples at once with KingFisher systems
- Samples can also be processed manually using a magnetic stand
- Isolate total RNA, as well as small RNAs such as microRNA (miRNA), from serum/plasma, whole blood, tissue, cell culture and urine using a phenol-free extraction (no organic extraction)
- Streamline protocols for numerous non-invasive biological samples as well as tissues and cell cultures
- Recover pure miRNA compatible with miRNA-Seq and qRT-PCR methods such as the TaqMan Advanced miRNA cDNA Synthesis Kit and other TaqMan advanced miRNA assays
- Download validated protocols for KingFisher purification instruments
MagMax mirVana Product Details
| Sample type | Multiple, including plasma/serum, whole blood, tissue, cell culture, urine | |
| Sample input volume |
| |
| Yield |
| |
| Elution volume | 50 µL | |
| Total processing time | 1.5–2 hr total time (20 min hands-on time) | |
| Reactions (rxn) | 96 rxn | |
| Shipping Condition | Room Temperature | |
| Kit contents: | Box 1 (store at -5 to -30°C):
| Box 2 (store at room temperature):
|
| Cat. No. | A27828 | |
Combine MagMAX with a KingFisher instrument to automate workflows
Use this kit with KingFisher automation to extract and purify RNA. The kit is designed for both low- and high-throughput isolation of true total RNA that covers the full range of RNAs present in cells, from small RNAs such as microRNA, to noncoding RNA, messenger RNA, and ribosomal RNA.
KingFisher sample workflow to isolate RNA
Extract total RNA from a wide range of sample types with MagMAX MirVana
Eliminate the headache of using “specialized” kits for each sample type by using a single solution with the MagMAX mirVana Total RNA Isolation Kit. Capable of processing a wide range of sample types, the kit is designed for both low- and high-throughput extraction of true total RNA. True total RNA includes the full range of RNAs present in cells such as microRNA, to noncoding RNA, messenger RNA or mRNA, and ribosomal RNA or rRNA. The phenol-free, magnetic bead format allows for automated RNA isolation workflows from the following samples:
Compatible Sample Types:
- Serum/plasma
- Whole blood
- Tissue
- Cell culture
- Urine
MagMAX magnetic bead–based technology for nucleic acid extraction
MagMAX products are designed to provide consistent and dependable nucleic acid extraction using silica–magnetic particle technology. Magnetic beads offer many benefits for isolating RNA from cells, tissue, or whole blood, and viral RNA from cell-free samples.
Technology at the core of the MagMAX:
- Offers higher RNA yields and more consistent results
- Binds RNA with greater efficiency than silica-membrane methods
- Elute RNA in as little as 20 µL of nuclease-free water to get more concentrated RNA
Video: How to isolate total RNA using magnetic bead–based technology
Learn how to achieve rapid, quantitative isolation of total RNA in both manual and automated workflows with the MagMAX mirVana Total RNA Isolation Kit.
Performance data
Application Notes that support performance data associated with the MagMAX mirVana Total RNA Isolation kit.
Click image to enlarge
Click image to enlarge
Figure 2. qRT-PCR results for tissue and cells. Total RNA, including small RNA, was isolated from mouse tissue and cells using the mirVana miRNA Isolation Kit or the MagMAX-96 mirVana Total RNA Isolation Kit. For the MagMAX-96 mirVana kit, RNA was isolated manually or using one of our automated instruments (MagMAX Express-96, KingFisher-Flex, or KingFisher-Duo).
For qRT-PCR analylsis, cDNA was generated using equal volumes RNA input from each sample (diluted 1:10 for miRNA) and the High-Capacity cDNA Reverse Transcription Kit for mRNA and the TaqMan MicroRNA Reverse Transcription Kit and specific primers for miRNA. qPCR reactions were then performed on 2 µL of cDNA using specific TaqMan Assays and TaqMan Universal Master Mix II (no UNG). Ct values were averaged and then graphed by target.
Click image to enlarge
Figure 3. Results with TaqMan Advanced miRNA System. Total RNA, including small RNA, was isolated from mouse liver, HeLa cells, and human plasma using the mirVana miRNA Isolation Kit, mirVana PARIS miRNA Isolation Kit, or MagMAX-96 mirVana Total RNA Isolation Kit.
For qRT-PCR analylsis, cDNA was generated using equal volumes RNA input from each sample (diluted 1:10 for liver and HeLa) and the TaqMan Advanced cDNA Synthesis Kit and protocol. qPCR reactions were then performed on 2 µL of cDNA using the specific TaqMan Advanced miRNA Assays and TaqMan FAST Advanced Master Mix and fast cycling conditions. Ct values were averaged and then graphed by target.
Click image to enlarge
Figure 4. qRT-PCR results for biofluids. Total RNA, including small RNA, was isolated from serum, plasma, and urine using the mirVana PARIS kit or the MagMAX-96 mirVana Total RNA Isolation Kit. For the MagMAX-96 mirVana kit, RNA was isolated manually or using one of our automated instruments (MagMAX Express-96, KingFisher-Flex, or KingFisher-Duo).
For qRT-PCR analylsis, cDNA was generated using equal volumes RNA input from each sample and the High-Capacity cDNA Reverse Transcription Kit for mRNA and the TaqMan MicroRNA Reverse Transcription Kit and specific primers for miRNA. qPCR reactions were then performed on 2 µL of cDNA using specific TaqMan Assays and TaqMan Universal Master Mix II (no UNG). Ct values were averaged and then graphed by target.
MagMax mirVana Product Details
| Sample type | Multiple, including plasma/serum, whole blood, tissue, cell culture, urine | |
| Sample input volume |
| |
| Yield |
| |
| Elution volume | 50 µL | |
| Total processing time | 1.5–2 hr total time (20 min hands-on time) | |
| Reactions (rxn) | 96 rxn | |
| Shipping Condition | Room Temperature | |
| Kit contents: | Box 1 (store at -5 to -30°C):
| Box 2 (store at room temperature):
|
| Cat. No. | A27828 | |
Combine MagMAX with a KingFisher instrument to automate workflows
Use this kit with KingFisher automation to extract and purify RNA. The kit is designed for both low- and high-throughput isolation of true total RNA that covers the full range of RNAs present in cells, from small RNAs such as microRNA, to noncoding RNA, messenger RNA, and ribosomal RNA.
KingFisher sample workflow to isolate RNA
Extract total RNA from a wide range of sample types with MagMAX MirVana
Eliminate the headache of using “specialized” kits for each sample type by using a single solution with the MagMAX mirVana Total RNA Isolation Kit. Capable of processing a wide range of sample types, the kit is designed for both low- and high-throughput extraction of true total RNA. True total RNA includes the full range of RNAs present in cells such as microRNA, to noncoding RNA, messenger RNA or mRNA, and ribosomal RNA or rRNA. The phenol-free, magnetic bead format allows for automated RNA isolation workflows from the following samples:
Compatible Sample Types:
- Serum/plasma
- Whole blood
- Tissue
- Cell culture
- Urine
MagMAX magnetic bead–based technology for nucleic acid extraction
MagMAX products are designed to provide consistent and dependable nucleic acid extraction using silica–magnetic particle technology. Magnetic beads offer many benefits for isolating RNA from cells, tissue, or whole blood, and viral RNA from cell-free samples.
Technology at the core of the MagMAX:
- Offers higher RNA yields and more consistent results
- Binds RNA with greater efficiency than silica-membrane methods
- Elute RNA in as little as 20 µL of nuclease-free water to get more concentrated RNA
Video: How to isolate total RNA using magnetic bead–based technology
Learn how to achieve rapid, quantitative isolation of total RNA in both manual and automated workflows with the MagMAX mirVana Total RNA Isolation Kit.
Performance data
Application Notes that support performance data associated with the MagMAX mirVana Total RNA Isolation kit.
Click image to enlarge
Figure 1. RNA quality on the Agilent Bioanalyzer. Total RNA, including small RNA, was isolated from 6 different tissue types (A: Mouse Brain; B: Mouse Heart; C: Mouse Liver; D: Mouse Spleen; E: HeLa cells; and F: Jurkat cells) using two different isolations methods and kits. Results shown in blue are for RNA isolated using the mirVana miRNA Isolation Kit and results shown in red are for RNA isolated using the MagMAX-96 mirVana Total RNA Isolation Kit that provides equivalent or improved results.
Click image to enlarge
Figure 2. qRT-PCR results for tissue and cells. Total RNA, including small RNA, was isolated from mouse tissue and cells using the mirVana miRNA Isolation Kit or the MagMAX-96 mirVana Total RNA Isolation Kit. For the MagMAX-96 mirVana kit, RNA was isolated manually or using one of our automated instruments (MagMAX Express-96, KingFisher-Flex, or KingFisher-Duo).
For qRT-PCR analylsis, cDNA was generated using equal volumes RNA input from each sample (diluted 1:10 for miRNA) and the High-Capacity cDNA Reverse Transcription Kit for mRNA and the TaqMan MicroRNA Reverse Transcription Kit and specific primers for miRNA. qPCR reactions were then performed on 2 µL of cDNA using specific TaqMan Assays and TaqMan Universal Master Mix II (no UNG). Ct values were averaged and then graphed by target.
Click image to enlarge
Figure 3. Results with TaqMan Advanced miRNA System. Total RNA, including small RNA, was isolated from mouse liver, HeLa cells, and human plasma using the mirVana miRNA Isolation Kit, mirVana PARIS miRNA Isolation Kit, or MagMAX-96 mirVana Total RNA Isolation Kit.
For qRT-PCR analylsis, cDNA was generated using equal volumes RNA input from each sample (diluted 1:10 for liver and HeLa) and the TaqMan Advanced cDNA Synthesis Kit and protocol. qPCR reactions were then performed on 2 µL of cDNA using the specific TaqMan Advanced miRNA Assays and TaqMan FAST Advanced Master Mix and fast cycling conditions. Ct values were averaged and then graphed by target.
Click image to enlarge
Figure 4. qRT-PCR results for biofluids. Total RNA, including small RNA, was isolated from serum, plasma, and urine using the mirVana PARIS kit or the MagMAX-96 mirVana Total RNA Isolation Kit. For the MagMAX-96 mirVana kit, RNA was isolated manually or using one of our automated instruments (MagMAX Express-96, KingFisher-Flex, or KingFisher-Duo).
For qRT-PCR analylsis, cDNA was generated using equal volumes RNA input from each sample and the High-Capacity cDNA Reverse Transcription Kit for mRNA and the TaqMan MicroRNA Reverse Transcription Kit and specific primers for miRNA. qPCR reactions were then performed on 2 µL of cDNA using specific TaqMan Assays and TaqMan Universal Master Mix II (no UNG). Ct values were averaged and then graphed by target.
Ordering information
Just like all Applied Biosystems MagMAX kits, the mirVana total RNA isolation kits are optimized for KingFisher instruments.
Nucleic Acid Isolation Resources
Application note
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