ELISA Buffers and Reagents

SuperBlock Blocking Bufferis a protein-based formulation that provides fast and effective blocking for ELISA, immunohistochemistry (IHC) and western blot analysis. It does not contain any immunoglobulins, albumin, or endogenous biotin, is compatible with streptavidin system, and yields a high signal-to-noise ratio. It may be used as a protein stabilizer for drying antigen or antibody-coated microplates.

SuperBlock Blocking Buffer is available in powder form and a ready-to-use solution. See selection guide (Tab 1) to choose for optimal reagent for your experiment.  

Protein-Free Blocking Buffer contains a protein-free compound and is highly effective at blocking ELISA microplates, membranes, and glass slide arrays. These buffers are free of biotin and are compatible with antibodies and avidin/biotin systems. They can be used to minimize or eliminate cross-reactivity associated with protein-blocking buffers and interference from glycosylation.

Protein-Free Blocking Buffers are optimized and validated in many protein methods to provide high signal-to-noise ratio and are supplied in a convenient ready-to-use format. See Figure 1 for comparison of Protein-Free Blocking Buffers and selection guide (Tab 1) to choose the suitable buffer for your experiment.

StartingBlock Blocking Buffer contains a single purified protein for instant blocking of western blot and ELISA assays especially chemiluminescent and fluorescent-based. These buffers provide broad compatibility with a wide range of antibodies and antibody combinations and are often used as a replacement of milk-based blocking buffers exhibiting high background or masking antigen-antibody binding. The blocker is serum-, albumin- and biotin-free and therefore is compatible with biotin/avidin systems.

StartingBlock Blocking Buffers are available as ready-to-use solution in PBS or TBS with and without Tween-20. See selection guide (Tab 1) to choose the best reagent for your experiment.  

Figure 1. Performance comparison between our Protein-Free Blocking Buffer and other commercial alternatives. Multiplex protein arrays were created by spotting 12 cytokine capture antibodies per well of 96-well microplates. The ELISA plates were then blocked with the indicated blocking buffers, probed with HRP-conjugated secondary antibody (no samples) and the background for each well was determined by chemiluminescence. Error bars represent the standard deviation for triplicate microplate wells. The graph shows that our Protein-Free Blocking Buffer exhibits less background than other commercial alternatives.

Blocker Caseincontains a single purified protein for blocking excess binding sites in ELISA, western blot, IHC, and nucleic acid detection methods. It provides fewer chances of cross-reactions with assay components and is a good alternative when high background or antigen-antibody masking is seen with non-fat milk blockers.

Blocker Casein is formulated in either PBS or TBS with 1% (w/v) casein and is available in a ready-to-use format. See selection guide (Tab 1) to choose this reagent for your experiment.  

Blocker BSA contains a single purified protein for blocking excess binding sites in western blot and ELISA, especially for targeting phospho-proteins. It provides fewer chances of cross-reactions with assay components and is a good alternative to non-fat milk blockers. It is devoid of endogenous biotin and is compatible with biotin/avidin systems. It may be used as a protein stabilizer for drying antigen or antibody-coated microplates, or used a diluent for storing used antibody solutions.

Blocker BSA is available in a ready-to-use format in either PBS or TBS with 10% (w/v) bovine serum albumin (BSA). See selection guide (Tab 1) to choose this reagent for your experiment.  

Fish Serum Blocking Buffer contains steelhead salmon serum and is an ideal blocking agent in ELISA, western blotting, immunohistochemistry, and other immunochemical detection methods involving mammalian samples, resulting in little to no background.  In addition, it is effective at stabilizing solutions and samples used for antibody-binding interactions and may be used as a diluent for antibodies.

Fish Serum Blocking Buffer is filtered and stabilized in PBS for compatibility with most assay systems and is supplied in a ready-to-use format. See selection guide (Tab 1) to choose this reagent for your experiment.  

View the instructional video demonstrating the workflow of a typical ELISA experiment using Invitrogen ELISA kits and reagents. Please make sure to carefully read and review your ELISA kit’s specific instruction sheet, located inside the package, for detailed information before beginning the procedure.

Explore our webinar around ELISA best practices for protein quantification. Learn how to analyze and interpret ELISA data, identify how to troubleshoot your ELISA experiment with useful tips and tricks to help you get reliable and accurate results.

ELISA Kits and Components

Learn about our ELISA kit formats including complete, ready-to-use kits as well as preoptimized reagents to design your own assay.

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ELISA Enzyme Substrates

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ELISA Instrumentation and Equipment

We offer centrifuges, cold storage equipment, dispensers, incubator/shakers, microplates, pipettes and tips, and microplate readers and washers to help meet all your ELISA needs.

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ELISA Microplates and Plasticware

Choose from an array of microplates with a variety of surfaces optimized to capture the biomolecule of your choice.

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