Table 13.2 Spectral properties of some lipid probes.
Fluorophore Labels for Lipid Probes
| ||||
|---|---|---|---|---|
| Spectral Property | Pyrene | DPH | NBD | BODIPY FL |
| Ex/Em (nm) * | 340/376 | 360/430 | 470/530 | 507/513 |
| QY (τ) † | 0.6 (>100 nanoseconds) | 0.8 (4–8 nanoseconds) | 0.32 (5–10 nanoseconds) | 0.9 (6 nanoseconds) |
| Concentration dependence | Excimer emission (~470 nm) at high concentrations. | Self-quenched at high concentrations. | Self-quenched at high concentrations. | Excimer emission (~620 nm) at high concentrations. |
| Environmental sensitivity | Very sensitive to quenching by oxygen. Essentially nonfluorescent in water. | Essentially nonfluorescent in water. | Essentially nonfluorescent in water. | Relatively insensitive. Strongly fluorescent in both aqueous and lipid environments. |
| * Typical fluorescence excitation and emission maxima for membrane-intercalated probes. † QY = fluorescence quantum yield; τ = fluorescence decay lifetime. Typical values for membrane-intercalated probes are listed. These values may show significant environment-dependent variations. | ||||
For Research Use Only. Not for use in diagnostic procedures.