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Table 12.2 Spectral characteristics of the MitoTracker probes.
| Cat. No. | MitoTracker Probe | Abs * (nm) | Em * (nm) | Oxidation State |
|---|---|---|---|---|
| M7514 | MitoTracker Green FM † | 490 | 516 | NA |
| M7510 | MitoTracker Orange CMTMRos | 551 | 576 | Oxidized |
| M7511 | MitoTracker Orange CM-H2TMRos | 551 ‡ | 576 ‡ | Reduced |
| M7512 | MitoTracker Red CMXRos | 578 | 599 | Oxidized |
| M7513 | MitoTracker Red CM-H2XRos | 578 ‡ | 599 ‡ | Reduced |
| M22425 | MitoTracker Red FM | 581 | 644 | NA |
| M22426 | MitoTracker Deep Red FM | 644 | 665 | NA |
| * Absorption (Abs) and fluorescence emission (Em) maxima, determined in methanol; values may vary somewhat in cellular environments. † MitoTracker Green FM is nonfluorescent in aqueous environments. ‡ These reduced MitoTracker probes are not fluorescent until oxidized. NA = Not applicable. | ||||
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