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Table 8.5 Molecular Probes ChromaTide and aha labeled nucleotides.
| Cat. No. | dUTP, aha-dUTP, aha-dCTP, OBEA-dCTP or UTP Nucleotide * | Ex/Em † | Applications ‡ |
|---|---|---|---|
| Nonfluorescent | |||
| B32766 | Biotin-aha-dUTP | NA | RT |
| B32772 | Biotin-aha-dCTP | NA | RT |
| Blue Fluorescence | |||
| C7612 | Cascade Blue-7-dUTP | 400/420 | PCR, TDT, RP |
| Green Fluorescence | |||
| C11397 | Alexa Fluor 488-5-dUTP | 490/520 | PCR, TDT, RP, RT, NT |
| C7604 | Fluorescein-12-dUTP | 495/525 | PCR, TDT, RP, NT |
| C7614 | BODIPY FL-14-dUTP | 505/515 | PCR, TDT, RP |
| F32767 | Fluorescein-aha-dUTP | 495/525 | RT |
| C21555 | Alexa Fluor 488-7-OBEA-dCTP | 490/520 | RT, NT |
| C11403 | Alexa Fluor 488-5-UTP | 490/520 | SP6, T3, T7 |
| Yellow Fluorescence | |||
| C11398 | Alexa Fluor 532-5-dUTP | 525/550 | PCR, TDT, RP, RT, NT |
| Orange Fluorescence | |||
| C7606MP | Tetramethylrhodamine-6-dUTP | 550/570 | PCR, TDT, RP |
| C11401 | Alexa Fluor 546-14-dUTP | 555/570 | PCR, TDT, RP, RT, NT |
| A32762 | Alexa Fluor 555-aha-dUTP | 555/570 | RT |
| A32770 | Alexa Fluor 555-aha-dCTP | 555/570 | RT |
| C21556 | Alexa Fluor 546-16-OBEA-dCTP | 555/570 | RT, NT |
| C11404 | Alexa Fluor 546-14-UTP | 555/570 | SP6, T3, T7 |
| Red Fluorescence | |||
| C11399 | Alexa Fluor 568-5-dUTP | 575/600 | PCR, TDT, RP, NT |
| C7631 | Texas Red-12-dUTP | 595/610 | PCR, TDT, RP |
| C11400 | Alexa Fluor 594-5-dUTP | 590/615 | PCR, TDT, RP, NT |
| Far-Red Fluorescence | |||
| A32763 | Alexa Fluor 647-aha-dUTP | 650/670 | RT |
| A32771 | Alexa Fluor 647-aha-dCTP | 650/670 | RT |
| C21559 | Alexa Fluor 647-12-OBEA-dCTP | 650/670 | RT, NT |
| * All products are supplied as either 25 µL (dUTPs and UTPs) or 50 µL (dCTPs) of a 1 mM solution in TE buffer. † Excitation (Ex) and emission (Em) maxima, in nm, for the labeled nucleotide. ‡ Except where otherwise noted, the following applications were tested: PCR, Taq polymerase for DNA amplification; TDT, terminal deoxynucleotidyl transferase for 3'-end labeling of dsDNA; RP, Klenow polymerase for labeling DNA by random hexamer priming; RT, murine leukemia virus (MLV) reverse transcriptase for synthesizing DNA from an RNA template; NT, nick translation using DNase I and DNA polymerase I. ChromaTide UTP nucleotides were incorporated into RNA by standard transcription reactions using the indicated RNA polymerases (SP6, T3, T7). We also offer unlabeled aha-dUTP (50 µL of a 50 mM solution in TE buffer, A32761) and unlabeled aha-dCTP (50 µL of a 50 mM solution in TE buffer, A32769; 500 µL of a 2 mM solution in TE buffer, A32768). | |||
For Research Use Only. Not for use in diagnostic procedures.