Table 14.2 Fluorescence characteristics of NeuroTrace fluorescent Nissl stains.
| Cat. No. | Fluorescent Color | Ex * | Em * | |||||
|---|---|---|---|---|---|---|---|---|
| 405 ± 20 445 ≥450 | 485 ± 11 505 535 ± 17.5 | 510 ± 11.5 540 ≥550 | 560 ± 20 590 610 ± 10 | 640 ± 10 660 682 ± 11 | ||||
| N21479 | Blue | 435 | 455 |
+++
|
+
|
–
|
–
|
–
|
| N21480 | Green | 500 | 525 |
–
|
+++
|
+++
|
–
|
–
|
| N21481 | Yellow | 515 | 535 |
–
|
+++
|
+++
|
+++
|
–
|
| N21482 | Red | 530 | 615 |
–
|
–
|
++
|
+++
|
–
|
| N21483 | Deep red | 640 | 660 |
–
|
–
|
– ‡
|
–
|
++
|
| * Approximate fluorescence excitation (Ex) and emission (Em) maxima, in nm. † Spectral characteristics in nm of the excitation filter, dichroic mirror and emission filter are shown top to bottom for each filter set. Relative signal strength for each filter combination is indicated with pluses and minuses. ‡ High background fluorescence. | ||||||||
For Research Use Only. Not for use in diagnostic procedures.