Table 4.2 Selected haptenylation reagents and their anti-hapten antibodies.
| Cat. No. | Preferred Reactive Hapten(s) | Unlabeled and Labeled Anti-Hapten Antibodies (Cat. No.) * |
|---|---|---|
| A30000 A30100 | Alexa Fluor 405, SE | Anti–Alexa Fluor 405/Cascade Blue dye (A5760) |
| A20000 A20100 A30005 A30052 | Alexa Fluor 488, SE Alexa Fluor 488, SE Alexa Fluor 488, 5-TFP Alexa Fluor 488, 5-SDP | Anti–Alexa Fluor 488 dye (A11094) |
| A2952 | 3-Amino-3-deoxydigoxigenin hemisuccinamide, SE | Anti-digoxigenin (available from other suppliers) |
| B1582 B1606 B6353 B6352 | Biotin-X, SE Biotin-XX, SE Biotin-X, SSE Biotin-XX, SSE | Anti-biotin (03-3700) |
| D6102 B10006 | BODIPY FL-X, SE BODIPY FL, STP ester | Anti–BODIPY FL dye (A5770) † |
| C2284 | Cascade Blue acetyl azide | Anti–Alexa Fluor 405/Cascade Blue dye (A5760) |
| D6104 | Dansyl-X, SE | Anti-dansyl (A6398) |
| D2248 B2604 | DNP-X, SE DNP-X–biocytin-X, SE | Anti-DNP (A6423, A6430, A6435, A11097) |
| F2181 F6130 | Fluorescein 5(6)-SFX Fluorescein-EX, SE | Anti–fluorescein/Oregon Green dye (A889, A982, A6413, A6421, A11090, A11091, A11095, A21253) † |
| L1338 | Lucifer yellow iodoacetamide | Anti–lucifer yellow dye (A5750, A5751) |
| O6185 | Oregon Green 488-X, SE | Anti–fluorescein/Oregon Green dye (A889, A982, A6413, A6421, A11090, A11091, A11095, A21253) † |
| T6105 | 5(6)-TAMRA-X, SE | Anti-tetramethylrhodamine (A6397), anti–Texas Red dye (A6399) † |
| R6160 | Rhodamine Red-X, SE | Anti-tetramethylrhodamine (A6397), anti–Texas Red dye (A6399) † |
| T6134 T20175 | Texas Red-X, SE | Anti-tetramethylrhodamine (A6397), anti–Texas Red dye (A6399) † |
| * See Anti-Dye and Anti-Hapten Antibodies—Section 7.4 for a description of these anti-hapten antibodies. † Both the anti-tetramethylrhodamine and the anti–Texas Red dye antibodies crossreact with tetramethylrhodamine, Lissamine rhodamine, Rhodamine Red and Texas Red fluorophores. Therefore, these fluorophores should not be used simultaneously to generate separate signals in a multicolor experiment. Similarly, the anti–BODIPY FL dye antibody may crossreact with other BODIPY dyes, and the anti–fluorescein/Oregon Green dye antibody crossreacts with both fluorescein and Oregon Green dyes. | ||
For Research Use Only. Not for use in diagnostic procedures.