High-fidelity PCR enzymes are used for applications requiring high accuracy during DNA amplification such as cloning, sequencing or mutagenesis. Thermo Scientific Phusion high-fidelity DNA polymerases were created by fusing a dsDNA-binding domain to a Pyrococcus polymerase–like proofreading enzyme. Due to this unique protein fusion engineering, Phusion DNA Polymerases have very low error rates, fast extension rates, and high inhibitor tolerance.
Find the right Phusion high-fidelity DNA polymerase format for your application
Phusion high-fidelity DNA polymerases are available in a variety of formats. Please reference the table below to find an appropriate Phusion format for your application.
| NEWPhusion Plus DNA Polymerase | Phusion High-Fidelity DNA Polymerase | Phusion Hot Start II DNA Polymerase | Phusion Flash High-Fidelity PCR Master Mix | Phusion U Hot Start DNA Polymerase | Phusion U Multiplex PCR Master Mix | |
|---|---|---|---|---|---|---|
| Fidelity (vs. Taq enzyme) | >100x | 52x | 52x | 25x | 25x | 25x |
| Tm calculation required | No | Yes | Yes | Yes | Yes | Yes |
| Enhanced specificity (hot-start version) | Yes | No | Yes | Yes | Yes | Yes |
| Amplification length | Up to 20 kb | Up to 20 kb | Up to 20 kb | Up to 20 kb | Up to 20 kb | Up to 2.5 kb |
| Speed | 15–30 sec/kb | 15–30 sec/kb | 15–30 sec/kb | <15 sec/kb | 15–30 sec/kb | 15–30 sec/kb |
| GC-rich format | Yes (new GC enhanced) | Yes | Yes | No | Yes | No |
| dUTP incorporation | No | No | No | No | Yes | Yes |
| Designed for multiplex PCR | No | No | No | No | No | Yes, up to 20 targets |
| Stand-alone enzyme format* | ||||||
| 2x master mix format* |
* All stand-alone enzymes are supplied with their reaction buffers. The 2x master mix formats include all necessary PCR components except the template and primers.
** Contains green loading dyes and a density reagent for direct gel loading of PCR products
Ordering information for Phusion high-fidelity DNA polymerases
Do you need a DNA polymerase with higher fidelity and/or performance than Phusion enzymes? Discover Invitrogen Platinum SuperFi II DNA Polymerasewith >300x Taq fidelity and a simplified PCR protocol.
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