Phusion U Hot Start DNA Polymerases
| Catalog Number | Color | No. of Reactions |
|---|---|---|
| F555S | Colorless | 100 Reactions |
| F556L | Green | 500 Reactions |
| F556S | Green | 100 Reactions |
| F555L | Colorless | 500 Reactions |
In addition to the uracil usage possibility, Phusion U polymerase features all the superior properties of other Phusion DNA polymerases: great accuracy, speed, ability to amplify long amplicons up to 20 kb, and a high specificity with Affibody-based hot start. These features make Phusion U Hot Start DNA Polymerase an excellent choice for such important applications as amplification of bisulphite-converted or damaged DNA as well as use of carryover contamination control.
Phusion U Hot Start Green DNA Polymerase is a combination of Phusion U Hot Start DNA Polymerase and 5X Phusion Green buffers. The buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffer does not interfere with Phusion U polymerase performance and is compatible with downstream applications such as DNA sequencing, ligation, and restriction digestion.
Highlights
- Accuracy—high fidelity DNA polymerase (25X Taq)
- Uracil-tolerance—engineered to incorporate dUTP and amplify uracil containing templates
- Specificity—hot start for reduced nonspecific amplification and primer degradation
- Speed—short extension times (15–30 s/kb)
- Green format—permits direct loading of PCR products on gels
Applications
- Amplification of bisulphite-converted DNA
- Amplification of damaged or aged DNA
- Carry-over contamination control
- Uracil-excision based (USER) cloning methods
Using Phusion DNA polymerases
Annealing rules for Phusion DNA Polymerases are different from many common DNA polymerases (such as Taq DNA polymerases).
• 5X Phusion HF & GC Buffers
• DMSO
• 50 mM MgCl2 solution
Phusion HF Buffer and Phusion GC Buffers each provide 1.5 mM MgCl2 in the final 1X concentration.
Store at -20°C.