Phusion U Hot Start PCR Master Mix
| Catalog Number | No. of Reactions |
|---|---|
| F533S | 100 Reactions |
| F533L | 500 Reactions |
Phusion U DNA Polymerase is a novel engineered high-fidelity enzyme developed using fusion technology. Designed with a proprietary mutation in the dUTP binding pocket of Phusion polymerase, Phusion U polymerase is able to overcome important limitations of proofreading enzymes to incorporate dUTP and read through uracil present in DNA templates.
Phusion U polymerase features properties of Phusion DNA polymerases and offers the ability to amplify long amplicons up to 20 kb and a high specificity with affibody based hot start.
These features make Phusion U Hot Start DNA Polymerase an excellent choice for such applications as amplification of bisulfite-converted or damaged DNA as well as use of carryover contamination control.
Highlights
- High-fidelity DNA polymerase (25X Taq)
- Engineered to incorporate dUTP and amplify uracil containing templates
- Hot start for reduced nonspecific amplification and primer degradation
- Only template and primers need to be added for use
- Short extension times (15–30 s/kb)
Applications
- Amplification of bisulfite-converted DNA
- Amplification of damaged or aged DNA
- Carry-over contamination control
- Uracil-excision based (USER) cloning methods
Using Phusion DNA Polymerases
- Annealing rules for Phusion DNA polymerases are different from many common DNA polymerases (such as Taq DNA polymerases).
• 2X reaction buffer
• 3 mM MgCl2
• 400 μM each of dATP, dTTP, dCTP and dGTP
Master mix is supplied with nuclease-free water.
Store at -20°C.