Overview

Selectivity at your fingertips

Solve your chromatography separation challenges with our reversed phase (RP) HPLC and UHPLC columns.

Reversed phase HPLC and UHPLC columns offer a wide variety of stationary phases for the effective separation of your analytes. Reversed phase stationary phases are available in a range of surface chemistries that vary in the length and structure of their hydrocarbon chains. Our reversed phase column portfolio includes options for C18, C8, C4, C30, polar-embedded phases, and aromatic group phases like phenyl, biphenyl, phenyl-hexyl, and PFP. These stationary phases also come in different sorbents such as porous silica, solid core silica, and polymeric resin. By switching between different stationary phases, it is possible to control how strongly your molecules of interest are retained within your column.

Popular Thermo Scientific reversed phase HPLC and UHPLC column brands include:

Need to maximize your productivity and flexibility in method development? Thermo Scientific reversed phase HPLC columns are available in an array of chemistries to optimize separations and provide enhanced retention or changes in elution order. Reversed phase columns are one of the most popular modes in HPLC analysis.

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Learn more about our small molecule HPLC and UHPLC column brands

Hydrophobic column phases

C18 (U)HPLC ColumnsC8 (U)HPLC ColumnsC4 (U)HPLC Columns
C18 (U)HPLC ColumnsC8 (U)HPLC Columns
C4 (U)HPLC Columns
  • Strong hydrophobic retention that will separate most small molecules
  • The laboratory workhorse and most commonly used selectivity
  • Great starting column for new applications
  • Medium hydrophobic retention
  • Ideal for highly hydrophobic small molecule analyte   
  • Densely bonded phase for separating structurally different molecules such as planar and bulky analytes
  • Weak hydrophobic retention
  • Excellent retention and resolution of proteins and other biomolecules
  • Can also be used to separate very hydrophobic macromolecules
ColumnColumn AdvantagesColumn Compatibility
Hypersil GOLD C18 Selectivity

 

  • Endcapped, ultrapure, silica-based C18 column
  • Exceptional peak shape and resolution for HPLC and LC/MS analyses using gradient methods

Uniguard Guard Cartridge Hold  

UHPLC Filters

Hypersil GOLD VANQUISH C18

 

  • Endcapped, ultrapure, silica-based C18 column
  • Packed to withstand high-pressure applications for more viscous mobile phase compositions or higher flow rates

UHPLC Filters

Hypersil GOLD Peptide C18
  • Endcapped, ultrapure, silica based C18 column
  • Includes a peptide-based QC test, providing ultimate confidence
  • Unparalleled sequence coverage and higher peak capacity
 
Accucore C18

 

  • Endcapped, solid core, high-efficiency, high-throughput C18 column
  • Achieve UHPLC efficiency on any instrument
  • Rapid separation of complex samples
  • Increased sensitivity and peak capacity

Uniguard Guard Cartridge Holders

Accucore XL C18

 

  • Endcapped, solid core, high-efficiency, high-throughput C18 column
  • Larger particles for increasing throughput when using older methods or older systems
  • Increased sensitivity and efficiency

Uniguard Guard Cartridge Holders

 

Accucore Vanquish
C18+, 1.5 µm

 

  • 1.5 µm solid core particles, packed to withstand high-pressure applications
  • Rapid separation of complex samples
  • Increased sensitivity and peak capacity

UHPLC Filters

Acclaim 120 C18

 

  • Endcapped, high-surface-area, silica-based C18 column
  • Increased resolution and retention
  • High retention and resolution of complex mixtures

Acclaim Guard Holder and Coupler

Acclaim VANQUISH C18

 

  • Endcapped, high-surface-area, silica-based C18 column
  • Packed to withstand high-pressure applications for more viscous mobile phase compositions or higher flow rates
  • Increased resolution and retention
  • High retention and resolution of complex mixtures
UHPLC Filters
ColumnColumn AdvantagesColumn Compatibility
Hypersil GOLD C8

 

  • Endcapped, ultrapure, silica-based C8 column
  • Excellent peak shape and resolution
Uniguard Guard Cartridge Holders

 

UHPLC Filters

Acclaim 120 C8

 

  • Endcapped, high-surface-area, silica-based C8 column
  • Increased resolution and retention
  • High retention and resolution of complex mixtures
Acclaim Guard Holder and Coupler

 

Accucore C8

 

  • Endcapped, solid core, high-efficiency, high-throughput C8 column
  • Increased sensitivity and peak capacity
Uniguard Guard Cartridge Holders

 

Accucore XL C8

 

  • Endcapped, solid core, high-efficiency, high-throughput C18 column
  • Larger particles for increasing throughput when using older methods or older systems
  • Increased sensitivity and peak capacity
Uniguard Guard Cartridge Holders

 

ColumnColumn AdvantagesColumn Compatibility
Hypersil GOLD C4
  • Endcapped, ultrapure, silica-based C4 column
  • Ideal for large peptides due to silica purity
  • Excellent peak shape and resolution

Uniguard Guard Cartridge Holders

UHPLC Filters

Accucore 150-C4

  • Endcapped, solid core, high-efficiency, high-throughput C4 column
  • Large pore size provides better peak shapes when analyzing larger peptides
  • Increased sensitivity and peak capacity

 

Uniguard Guard Cartridge Holders

Polar-modified C18 column phases

Polar-Endcapped C18 (U)HPLC ColumnsAmide-Embedded C18 (U)HPLC ColumnsSulfonamide-Embedded C18 (U)HPLC Columns
Polar-Endcapped C18 (U)HPLC Columns
Amide-Embedded C18 (U)HPLC Columns
Sulfonamide-Embedded C18 (U)HPLC Columns
  • Endcapped C18 phase with secondary polar selectivity
  • Excellent for applications with mixed polar and non-polar analytes
  • Can withstand 100% water mobile phase
  • Polar-embedded C18 phase with amide-embedded functional group
  • Provides great retention and excellent peak shapes for polar bases
  • Can withstand 100% water mobile phase
  • Polar-embedded C18 phase with sulfonamide-embedded functional group
  • Provides great retention and excellent peak shapes for polar bases and polar acids
  • Can withstand 100% water mobile phase

 

ColumnColumn AdvantagesColumn Compatibility
Hypersil GOLD aQ C18 Polar-Endcapped

 

  • Polar-endcapped, ultrapure, silica-based C18 column
  • Exceptional peak shape and resolution for HPLC and LC/MS analyses using gradient methods

Uniguard Guard Cartridge Holders

UHPLC Filters

Hypersil GOLD VANQUISH
aQ

 

  • Polar-endcapped, ultrapure, silica-based C18 column
  • Packed to withstand high-pressure applications for more viscous mobile phase compositions or higher flow rates

UHPLC Filters

 

Accucore aQ
C18 Polar-Endcapped

 

  • Polar-endcapped, solid core, high-efficiency, high-throughput C18 column
  • Achieve UHPLC efficiency on any instrument
  • Rapid separation of complex samples
  • Increased sensitivity and peak capacity

Uniguard Guard Cartridge Holders

 

ColumnColumn AdvantagesColumn Compatibility
Accucore Polar Premium

 

  • Endcapped, solid core, high-efficiency, high-throughput, polar-embedded C18 column
  • Achieve UHPLC efficiency on any instrument
  • Rapid separation of complex samples
  • Increased sensitivity and peak capacity
Uniguard Guard Cartridge Holders

 

Acclaim Polar Advantage II (PA 2)

 

 

  • Endcapped, high-surface-area, silica-based, polar-embedded C18 column
  • Increased resolution and retention
  • High retention and resolution of complex mixtures
Acclaim Guard Holder and Coupler

 

Acclaim VANQUISH Polar Advantage II (PA 2)
  • Endcapped, high-surface-area, silica-based, polar-embedded C18 column
  • Packed to withstand high-pressure applications for more viscous mobile phase compositions or higher flow rates
  • Increased resolution and retention
  • High retention and resolution of complex mixtures
UHPLC Filters
ColumnColumn AdvantageColumn Compatibility

Acclaim Polar Advantage (PA)

  • Endcapped, high-surface-area, silica-based, polar-embedded C18 column 
  • Increased resolution and retention
  • High retention and resolution of complex mixtures

Acclaim Guard Holder and Coupler

Specialty hydrophobic column phases

Accucore RP-MS (U)HPLC ColumnsC30 (U)HPLC Columns

Accucore RP-MS (U)HPLC Columns

C30 (U)HPLC Columns
  • Densely-bonded proprietary hydrophobic selectivity phase that allows for geometrical separation as well as hydrophobic retention
  • C18-like selectivity
  • Excellent for MS applications where speed and sensitivity are of the essence

 

  • Large, bulky phase that combines hydrophobic selectivity and geometrical separations
  • C30 ligands bend over themselves when bonded onto the surface of silica packing material, forming pockets that create geometrical interactions with analytes
  • C18-like selectivity 
  • Ideal for the separation of longer-chained isomers such as fatty acids and vitamin K

Specialty hydrophobic column phases

ColumnColumn AdvantageColumn Compatibility

Accucore RP-MS

  • Endcapped, solid core, high-efficiency, high-throughput column
  • Achieve UHPLC efficiency on any instrument
  • Rapid separation of complex samples
  • Increased sensitivity and peak capacity

Uniguard Guard Cartridge Holders

Column Column AdvantageColumn Compatibility

Accucore C30

  • Endcapped, solid core, high-efficiency, high-throughput C30 column
  • Achieve UHPLC efficiency on any instrument
  • Rapid separation of complex samples
  • Increased sensitivity and peak capacity

Uniguard Guard Cartridge Holders

Acclaim C30

  • Endcapped, high-surface area, silica-based, polar-embedded C30 column 
  • High retention and resolution of complex mixtures

Acclaim Guard Holder and Coupler

Phenyl-based column phases

Phenyl and Phenyl-Hexyl (U)HPLC Columns

 

Biphenyl (U)HPLC Columns

 

PFP (Pentafluorophenyl) (U)HPLC Columns

 

Phenyl and Phenyl-Hexyl (U)HPLC Columns
Biphenyl (U)HPLC Columns
PFP (Pentafluorophenyl) (U)HPLC Columns
  • Moderately hydrophobic traditional phenyl phases that separate samples via aromatic interactions such as pi-pi stacking and dipol-dipol interactions
  • Orthogonal selectivity to traditional hydrophobic phases for challenging separations that require aromatic interactions
  • Excellent for separations of aromatic and moderately polar analytes

 

  • Strongly hydrophobic phenyl phase that separates samples via aromatic interactions such as pi-pi stacking and dipol-dipol interactions
  • Orthogonal selectivity to traditional hydrophobic phases for challenging separations that require aromatic and strong hydrophobic interactions
  • Ideal for small molecule pharmaceutical and forensic applications such as the analysis of drugs of abuse
  • Strong aromatic interaction phase that separates analytes through more permanent pi-pi stacking
  • Orthogonal selectivity to traditional hydrophobic phases for challenging separations that require strong aromatic interactions
  • Excellent for aromatic isomers as well as polar and halogenated aromatic compounds
Column

 

Column AdvantagesColumn Compatibility

 

Hypersil GOLD Phenyl

  • Endcapped, ultrapure, silica-based phenyl column
  • Exceptional peak shape and resolution for HPLC and LC/MS analyses using gradient methods

Uniguard Guard Cartridge Holders

 

 

Accucore Phenyl-Hexyl

 

  • Endcapped, solid core, high-efficiency, high-throughput phenyl-hexyl column
  • Achieve UHPLC efficiency on any instrument
  • Rapid separation of complex samples
  • Increased sensitivity and peak capacity

Uniguard Guard Cartridge Holders

 

 

Column

 

Column AdvantagesColumn Compatibility

 

Accucore Biphenyl
  • Endcapped, solid core, high-efficiency, high-throughput biphenyl column
  • Achieve UHPLC efficiency on any instrument
  • Rapid separation of complex samples
  • Increased sensitivity and peak capacity

Uniguard Guard Cartridge Holders

Column

Column Advantages

Column Compatibility

Hypersil GOLD PFP

  • Endcapped, ultrapure, silica-based PFP column
  • Exceptional peak shape and resolution for HPLC and LC/MS analyses using gradient methods

Uniguard Guard Cartridge Holders

Hypersil GOLD VANQUISH PFP

  • Endcapped, ultrapure, silica-based PFP column
  • Packed to withstand high-pressure applications for more viscous mobile phase compositions or higher flow rates

 

Uniguard Guard Cartridge Holders

Accucore PFP

  • Endcapped, solid core, high-efficiency, high-throughput PFP column
  • Achieve UHPLC efficiency on any instrument
  • Rapid separation of complex samples
  • Increased sensitivity and peak capacity

Uniguard Guard Cartridge Holders

HPLC systems and consumables

Sample preparation

* Required field

Need similar selectivity to C18 and C8 columns but less retention? C4 reversed phase HPLC columns provides outstanding sensitivity and high efficiency; and its shorter chain length and lower hydrophobicity make it useful for hydrophobic polypeptides and proteins.

Trying to separate aromatic isomers or halogenated compounds? PFP reversed phase HPLC columns offer alternative selectivity to C18 and are well suited to the selective analysis of halogenated and polar compounds containing hydroxyl, carboxyl, nitro, or other polar groups.

Need to retain polar compounds or use 100% aqueous mobile phase? These polar endcapped C18 reversed phase HPLC columns provide a controlled interaction mechanism by which polar analytes can be retained and exhibit superior stability towards highly aqueous mobile phase.

Style Sheet for Global Design System
Style Sheet for Komodo Tabs
Style Sheet for Global Design System
Style Sheet for Komodo Tabs
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