Comparison of fluorescence intensity of eFluor 450 dye with Pacific Blue. Human peripheral blood cells (PBMCs) were stained with CD45RA Monoclonal Antibody (HI100), eFluor 450 (purple line) or CD45RA Monoclonal Antibody (MEM-56), Pacific Blue (blue dotted line).
 |  |  |  |  |  |  |
| 2 | 405 | 450/50 | 405 | 445 | (in buffer) 1 | flow cytometry |
The eFluor 450 dye is a violet-excitable, organic dye that emits at 450 nm and has spectral and performance properties similar to Pacific Blue dye. It exhibits equal or better fluorescence intensity when compared to Horizon V500 dye or Pacific Blue dye in multicolor applications. Stability data shows eFluor 450 exhibits minimal loss of fluorescence, when cells are exposed to a formaldehyde fixative treatment for up to 24 hours.
We offer eFluor 450 dye conjugated to primary antibodies for use in flow cytometry.
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- Equal or better fluorescence intensity when compared to several other violet-excited organic dyes
- Stable fluorescence following aldehyde fixation
- Helps extend options for multicolor panel design to the violet laser
Stability of eFluor 450 dye. Mouse splenocytes were stained with CD4 Monoclonal Antibody (RM4-5), eFluor 450 and analyzed immediately (red line). The sample was then fixed in 2% formaldehyde overnight at 4°C and analyzed again (green line).
Additional resources
Flow cytometry protocols handbook
Protocols that fit your needs in flow cytometry ranging from sample preparation to numerous cell stimulation conditions, staining, immunophenotyping, and data analysis strategies.
Fluorophore and reagent selection guide for flow cytometry
A handy reference poster featuring the broad range of our available dyes and labeling reagents.
Imaging protocol handbook
Protocols that fit your needs in imaging ranging from sample and assay preparation to staining, labeling, and data analysis strategies.
Fluorophore and reagent selection guide for cell imaging
A tool for selecting the optimal fluorescent dyes for your Invitrogen EVOS cell imaging systems.
Cy™ is a trademark or registered trademark of GE Healthcare.
Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Brilliant Ultra Violet™ and Brilliant Violet™ are trademarks or registered trademarks of Becton, Dickinson and Company or its affiliates, and are used under license.
For Research Use Only. Not for use in diagnostic procedures.