Flow cytometry analysis of human mononuclear cells using Alexa Fluor 594 streptavidin. Human mononuclear cells were incubated with CD4, Mouse Anti-Human (Biotin), washed and then stained with Streptavidin, Alexa Fluor 594 conjugate. Cells were washed and analyzed by flow cytometry using 532 nm excitation.
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| 5 | 561/594 | Texas Red | 590 | 618 | (in buffer) 3 (in antifade) 4 | microscopy; flow cytometry; fluorescence microplate reader |
Invitrogen Alexa Fluor 594 dye is a bright, red-fluorescent dye that can be excited using the 561 nm or 594 nm laser lines. It is commonly used with Alexa Fluor 350, Alexa Fluor 488 and Alexa Fluor 647 dyes for multiplexing. For stable signal generation in imaging and flow cytometry, Alexa Fluor 594 dye is pH-insensitive over a wide molar range. In addition, it is used in a range of SRM applications including dSTORM, structured illumination microscopy (SIM), stimulated emission depletion (STED) microscopy and in two-photon excitation (TPE) microscopy.
Probes with high fluorescence quantum yield and high photostability allow detection of low-abundance biological structures with great sensitivity. Alexa Fluor 594 dye molecules can be attached to proteins at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection.
We offer Alexa Fluor 594 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling and detection. In addition, reactive dye forms and protein labeling kits are available that allows you to generate your own antibody conjugates or probes.
Search Alexa Fluor 594 secondary antibodies Protein Labeling Reagents Selection Guide (NHS ester, maleimide, etc.)
Additional products
- Antibody and protein labeling kits
- Search Alexa Fluor 594 primary antibodies
- Super-resolution microscopy (SRM) applications
- Cell and Neuronal Tracing
- Streptavidin conjugates for signal amplification
- Labeled nucleotides for hybridization probes
- Phalloidin conjugates for actin staining
- Tyramide conjugates for ultimate sensitivity
- Annexin V conjugates for apoptosis imaging
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Fluorescence microscopy of bovine endothelial cells. Microtubules of fixed bovine pulmonary artery endothelial cells localized with alpha-Tubulin Monoclonal Antibody , which was subsequently visualized with Goat anti-Mouse IgG Cross-Adsorbed Antibody, Alexa Fluor 350. Next, the F-actin was labeled with Alexa Fluor 594 Phalloidin. Finally, the cells were incubated with Wheat Germ Agglutinin, Alexa Fluor 488 Conjugate to stain components of endosomal pathways. The superimposed and pseudocolored images were acquired sequentially using bandpass filter sets appropriate for DAPI, the Texas Red dye and FITC, respectively.
Additional resources
Flow cytometry protocols handbook
Protocols that fit your needs in flow cytometry ranging from sample preparation to numerous cell stimulation conditions, staining, immunophenotyping, and data analysis strategies.
Fluorophore and reagent selection guide for flow cytometry
A handy reference poster featuring the broad range of our available dyes and labeling reagents.
Imaging protocol handbook
Protocols that fit your needs in imaging ranging from sample and assay preparation to staining, labeling, and data analysis strategies.
Fluorophore and reagent selection guide for cell imaging
A tool for selecting the optimal fluorescent dyes for your Invitrogen EVOS cell imaging systems.
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