Invitrogen

CD45RA Monoclonal Antibody (MEM-56), Pacific Blue™

Product Details

MHCD45RA28

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

Assay-dependent

View 5 publications 5 publications

Miscellaneous PubMed (Misc)

View 5 publications 5 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

MEM-56

Immunogen

Human CD45RA

Conjugate

Pacific Blue™ Pacific Blue™ Pacific Blue™

Excitation/Emission Max

404/455 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

purified

Storage buffer

PBS with 4mg/mL BSA

Contains

0.1% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10371901

Target Information

CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing and these isoforms differ in their extracellular domains, however, they share identical transmembrane and cytoplasmic domains. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 is expressed on cells of the human hematopoietic lineage, with the exception of mature red cells and is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Diseases associated with CD45 dysfunction include severe combined immunodeficiency and Hepatitis C.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

N⁶-methyladenosine mRNA marking promotes selective translation of regulons required for human erythropoiesis.

Nature communications

Kuppers DA,Arora S,Lim Y,Lim AR,Carter LM,Corrin PD,Plaisier CL,Basom R,Delrow JJ,Wang S,Hansen He H,Torok-Storb B,Hsieh AC,Paddison PJ

MHCD45RA28 was used in Flow Cytometry to conclude that m6A mRNA marks promote the translation of a network of genes required for human erythropoiesis.

Thu Oct 10 00:00:00 EDT 2019

T lymphocytes and fractalkine contribute to myocardial ischemia/reperfusion injury in patients.

The Journal of clinical investigation

Boag SE,Das R,Shmeleva EV,Bagnall A,Egred M,Howard N,Bennaceur K,Zaman A,Keavney B,Spyridopoulos I

MHCD45RA28 was used in flow cytometry to investigate the contribution of lymphocytes to primary percutaneous coronary intervention

Mon Aug 03 00:00:00 EDT 2015

Proteome-wide analysis of HIV-specific naive and memory CD4(+) T cells in unexposed blood donors.

The Journal of experimental medicine

Campion SL,Brodie TM,Fischer W,Korber BT,Rossetti A,Goonetilleke N,McMichael AJ,Sallusto F

MHCD45RA28 was used in immunocytochemistry to analyze HIV-specific naive and memory CD4(+) T cells through two methods.

Mon Jun 30 00:00:00 EDT 2014

Chronic unpredictable mild stress impairs erythrocyte immune function and changes T-lymphocyte subsets in a rat model of stress-induced depression.

Environmental toxicology and pharmacology

Guan SZ,Liu JW,Fang EF,Ng TB,Lian YL,Ge H

MHCD45RA28 was used in flow cytometry to examine the changes in erythrocytes and T-lymphocytes and to explore the mechanisms involved in stress-induced depression using a rat depression model.

Wed Jan 01 00:00:00 EST 2014

Expression of integrin α2 receptor in human cord blood CD34+CD38-CD90+ stem cells engrafting long-term in NOD/SCID-IL2Rγ(c) null mice.

Stem cells (Dayton, Ohio)

Wong WM,Sigvardsson M,Åstrand-Grundström I,Hogge D,Larsson J,Qian H,Ekblom M

MHCD45RA28 was used in flow cytometry to identify integrin α2 as a novel stem cell marker.

Fri Feb 01 00:00:00 EST 2013

CD4 and CD8 T cell immune activation during chronic HIV infection: roles of homeostasis, HIV, type I IFN, and IL-7.

Journal of immunology (Baltimore, Md. : 1950)

Catalfamo M,Wilhelm C,Tcheung L,Proschan M,Friesen T,Park JH,Adelsberger J,Baseler M,Maldarelli F,Davey R,Roby G,Rehm C,Lane C

MHCD45RA28 was used in flow cytometry to investigate the regulation of the CD4 and CD8 T cell pools during HIV infection.

Tue Feb 15 00:00:00 EST 2011

Th17 central memory T cells are reduced by FTY720 in patients with multiple sclerosis.

Neurology

Mehling M,Lindberg R,Raulf F,Kuhle J,Hess C,Kappos L,Brinkmann V

MHCD45RA28 was used in flow cytometry to determine the effect of FTY720 treatment on Th17 cells

Tue Aug 03 00:00:00 EDT 2010

Loss of FOXP3 expression in natural human CD4+CD25+ regulatory T cells upon repetitive in vitro stimulation.

European journal of immunology

Hoffmann P,Boeld TJ,Eder R,Huehn J,Floess S,Wieczorek G,Olek S,Dietmaier W,Andreesen R,Edinger M

MHCD45RA28 was used in Flow cytometry/Cell sorting to study the epigenetic regulation of the FOXP3 gene locus in regulatory T cells following repetitive in vitro stimulation.

Wed Apr 01 00:00:00 EDT 2009

Functional regulatory T cells accumulate in aged hosts and promote chronic infectious disease reactivation.

Journal of immunology (Baltimore, Md. : 1950)

Lages CS,Suffia I,Velilla PA,Huang B,Warshaw G,Hildeman DA,Belkaid Y,Chougnet C

MHCD45RA28 was used in flow cytometry to characterize Tregs from older subjects and mice.

Fri Aug 01 00:00:00 EDT 2008

Comparative study of regulatory T cell function of human CD25CD4 T cells from thymocytes, cord blood, and adult peripheral blood.

Clinical & developmental immunology

Fujimaki W,Takahashi N,Ohnuma K,Nagatsu M,Kurosawa H,Yoshida S,Dang NH,Uchiyama T,Morimoto C

MHCD45RA28 was used in flow cytometry to assess the regulatory function of human CD25+CD4+ T cells at various stages of maturity

Fri Jan 02 00:00:00 EST 2009

In vivo evaluation of human hematopoiesis through xenotransplantation of purified hematopoietic stem cells from umbilical cord blood.

Nature protocols

Park CY,Majeti R,Weissman IL

MHCD45RA28 was used in flow cytometry to develop a xenotransplantation system using human hematopoietic stem cells and mice

Wed Apr 01 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: CD45; CD45 antigen; L-CA; Leukocyte common antigen; Lyt-4; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Receptor-type tyrosine-protein phosphatase C; T100; T200; T200 glycoprotein; T200 leukocyte common antigen

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Gene Aliases: B220; CD45; CD45R; GP180; L-CA; LCA; LY5; PTPRC; T200

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UniProt ID: (Human) P08575

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Entrez Gene ID: (Human) 5788

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Function(s)

protein tyrosine phosphatase activity transmembrane receptor protein tyrosine phosphatase activity protein binding protein kinase binding protein phosphatase protein modifying enzyme

Process(es)

negative regulation of T cell mediated cytotoxicity negative regulation of cytokine-mediated signaling pathway hematopoietic progenitor cell differentiation immunoglobulin biosynthetic process negative regulation of protein kinase activity protein dephosphorylation negative regulation of cell adhesion involved in substrate-bound cell migration cell surface receptor signaling pathway dephosphorylation T cell differentiation positive regulation of B cell proliferation peptidyl-tyrosine dephosphorylation B cell proliferation positive regulation of T cell proliferation cell cycle phase transition positive regulation of protein kinase activity bone marrow development stem cell development T cell receptor signaling pathway B cell receptor signaling pathway positive regulation of antigen receptor-mediated signaling pathway release of sequestered calcium ion into cytosol defense response to virus regulation of cell cycle positive regulation of hematopoietic stem cell migration positive regulation of stem cell proliferation

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CD45RA Monoclonal Antibody (MEM-56), Pacific Blue™

Product Details

MHCD45RA28

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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