Invitrogen

CD45RA Monoclonal Antibody (HI100), eFluor™ 450, eBioscience™

CD45RA Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 7

CD45RA Antibody (48-0458-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD45RO FITC (Product # 11-0457-42) and Mouse IgG2b K Isotype Control eFluor® 450 (Product # 48-4732-82) (left) or Anti-Human CD45RA eFluor® 450 (right). Cells in the lymphocyte gate were used for analysis.

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Product Details

48-0458-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.5 µg)/test

View 20 publications 20 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG2b, kappa

Recommended Isotype Control

Mouse IgG2b kappa Isotype Control (eBMG2b), eFluor™ 450, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

HI100

Conjugate

eFluor™ 450 eFluor™ 450 eFluor™ 450

Excitation/Emission Max

405/445 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1272059

Product Specific Information

Description: The HI100 monoclonal antibody reacts with human CD45RA, a 220 kDa molecule expressed by subpopulations of CD4+ peripheral T lymphocytes, CD8+ peripheral T lymphocytes, and B cells. The CD45RA+ T cell populations are mainly naive/virgin allowing the use of HI100 mAb as a phenotypic marker to discriminate T cell subsets.

Applications Reported: This HI100 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This HI100 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral mononuclear cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing and these isoforms differ in their extracellular domains, however, they share identical transmembrane and cytoplasmic domains. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 is expressed on cells of the human hematopoietic lineage, with the exception of mature red cells and is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Diseases associated with CD45 dysfunction include severe combined immunodeficiency and Hepatitis C.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

SARS-CoV-2 mRNA vaccination elicits a robust and persistent T follicular helper cell response in humans.

Cell

Mudd PA,Minervina AA,Pogorelyy MV,Turner JS,Kim W,Kalaidina E,Petersen J,Schmitz AJ,Lei T,Haile A,Kirk AM,Mettelman RC,Crawford JC,Nguyen THO,Rowntree LC,Rosati E,Richards KA,Sant AJ,Klebert MK,Suessen T,Middleton WD,Wolf J,Teefey SA,O'Halloran JA,Presti RM,Kedzierska K,Rossjohn J,Thomas PG,Ellebedy AH

48-0458-42 was used in Flow Cytometry to evaluate the T cell receptor sequences and phenotype of SARS-CoV-2 vaccine-induced lymph node follicular helper CD4+ T cells.

Thu Feb 17 00:00:00 EST 2022

Heterogeneous disease-propagating stem cells in juvenile myelomonocytic leukemia.

The Journal of experimental medicine

Louka E,Povinelli B,Rodriguez-Meira A,Buck G,Wen WX,Wang G,Sousos N,Ashley N,Hamblin A,Booth CAG,Roy A,Elliott N,Iskander D,de la Fuente J,Fordham N,O'Byrne S,Inglott S,Norfo R,Salio M,Thongjuea S,Rao A,Roberts I,Mead AJ

Published figure using CD45RA monoclonal antibody (Product # 48-0458-42) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2021

Longitudinal high-throughput TCR repertoire profiling reveals the dynamics of T-cell memory formation after mild COVID-19 infection.

eLife

Minervina AA,Komech EA,Titov A,Bensouda Koraichi M,Rosati E,Mamedov IZ,Franke A,Efimov GA,Chudakov DM,Mora T,Walczak AM,Lebedev YB,Pogorelyy MV

48-0458-42 was used in Flow Cytometry to use longitudinal high-throughput T-cell receptor (TCR) sequencing to track changes in the T-cell repertoire following two mild cases of COVID-19.

Tue Jan 05 00:00:00 EST 2021

Differences in Maturation Status and Immune Phenotypes of Circulating Helios⁺ and Helios^- Tregs and Their Disrupted Correlations With Monocyte Subsets in Autoantibody-Positive T1D Individuals.

Frontiers in immunology

Zhang Y,Zhang J,Shi Y,Shen M,Lv H,Chen S,Feng Y,Chen H,Xu X,Yang T,Xu K

Published figure using CD45RA monoclonal antibody (Product # 48-0458-42) in Flow Cytometry

Wed Sep 15 00:00:00 EDT 2021

Single-Cell Analyses Reveal Megakaryocyte-Biased Hematopoiesis in Myelofibrosis and Identify Mutant Clone-Specific Targets.

Molecular cell

Psaila B,Wang G,Rodriguez-Meira A,Li R,Heuston EF,Murphy L,Yee D,Hitchcock IS,Sousos N,O'Sullivan J,Anderson S,Senis YA,Weinberg OK,Calicchio ML,Iskander D,Royston D,Milojkovic D,Roberts I,Bodine DM,Thongjuea S,Mead AJ

Published figure using CD45RA monoclonal antibody (Product # 48-0458-42) in Flow Cytometry

Thu May 07 00:00:00 EDT 2020

The downregulated membrane expression of CD18 in CD34⁺ cells defines a primitive population of human hematopoietic stem cells.

Stem cell research & therapy

Mesa-Núñez C,Leon-Rico D,Aldea M,Damián C,Sanchez-Baltasar R,Sanchez R,Alberquilla O,Segovia JC,Bueren JA,Almarza E

Published figure using CD45RA monoclonal antibody (Product # 48-0458-42) in Flow Cytometry

Tue Apr 28 00:00:00 EDT 2020

MicroRNA‑155 inhibits the proliferation of CD8+ T cells via upregulating regulatory T cells in vitiligo.

Molecular medicine reports

Lv M,Li Z,Liu J,Lin F,Zhang Q,Li Z,Wang Y,Wang K,Xu Y

Published figure using CD45RA monoclonal antibody (Product # 48-0458-42) in Flow Cytometry

Tue Oct 01 00:00:00 EDT 2019

Targeting enhancer switching overcomes non-genetic drug resistance in acute myeloid leukaemia.

Nature communications

Bell CC,Fennell KA,Chan YC,Rambow F,Yeung MM,Vassiliadis D,Lara L,Yeh P,Martelotto LG,Rogiers A,Kremer BE,Barbash O,Mohammad HP,Johanson TM,Burr ML,Dhar A,Karpinich N,Tian L,Tyler DS,MacPherson L,Shi J,Pinnawala N,Yew Fong C,Papenfuss AT,Grimmond SM,Dawson SJ,Allan RS,Kruger RG,Vakoc CR,Goode DL,Naik SH,Gilan O,Lam EYN,Marine JC,Prinjha RK,Dawson MA

Published figure using CD45RA monoclonal antibody (Product # 48-0458-42) in Flow Cytometry

Thu Jun 20 00:00:00 EDT 2019

Targeting a CAR to the TRAC locus with CRISPR/Cas9 enhances tumour rejection.

Nature

Eyquem J,Mansilla-Soto J,Giavridis T,van der Stegen SJ,Hamieh M,Cunanan KM,Odak A,Gönen M,Sadelain M

Published figure using CD45RA monoclonal antibody (Product # 48-0458-42) in Flow Cytometry

Thu Mar 02 00:00:00 EST 2017

Human CD4⁺ T Cell Responses to an Attenuated Tetravalent Dengue Vaccine Parallel Those Induced by Natural Infection in Magnitude, HLA Restriction, and Antigen Specificity.

Journal of virology

Angelo MA,Grifoni A,O'Rourke PH,Sidney J,Paul S,Peters B,de Silva AD,Phillips E,Mallal S,Diehl SA,Kirkpatrick BD,Whitehead SS,Durbin AP,Sette A,Weiskopf D

48-0458 was used in Flow cytometry/Cell sorting to study the CD4 T cell responses elicited by a tetravalent live attenuated dengue vaccine and show that they resemble responses seen in humans naturally exposed to dengue virus.

Wed Mar 01 00:00:00 EST 2017

The distribution and function of human memory T cell subsets in lung cancer.

Immunologic research

Sheng SY,Gu Y,Lu CG,Zou JY,Hong H,Wang R

Published figure using CD45RA monoclonal antibody (Product # 48-0458-42) in Flow Cytometry

Thu Jun 01 00:00:00 EDT 2017

TIGIT expressing CD4+T cells represent a tumor-supportive T cell subset in chronic lymphocytic leukemia.

Oncoimmunology

Catakovic K,Gassner FJ,Ratswohl C,Zaborsky N,Rebhandl S,Schubert M,Steiner M,Gutjahr JC,Pleyer L,Egle A,Hartmann TN,Greil R,Geisberger R

Published figure using CD45RA monoclonal antibody (Product # 48-0458-42) in Flow Cytometry

Sat Jan 29 00:00:00 EST 2022

A Quantitative Analysis of Complexity of Human Pathogen-Specific CD4 T Cell Responses in Healthy M. tuberculosis Infected South Africans.

PLoS pathogens

Lindestam Arlehamn CS,McKinney DM,Carpenter C,Paul S,Rozot V,Makgotlho E,Gregg Y,van Rooyen M,Ernst JD,Hatherill M,Hanekom WA,Peters B,Scriba TJ,Sette A

48-0458 was used in Flow cytometry/Cell sorting to estimate the breadth of T cell responses for the first time in the context of an infection and human population setting.

Fri Jul 01 00:00:00 EDT 2016

Immunodominant Dengue Virus-Specific CD8+ T Cell Responses Are Associated with a Memory PD-1+ Phenotype.

Journal of virology

de Alwis R,Bangs DJ,Angelo MA,Cerpas C,Fernando A,Sidney J,Peters B,Gresh L,Balmaseda A,de Silva AD,Harris E,Sette A,Weiskopf D

48-0458 was used in Flow cytometry/Cell sorting to define DENV-specific CD8(+)T cell responses in general and those of HLA alleles associated with dominant responses.

Sun May 01 00:00:00 EDT 2016

The human CD8+ T cell responses induced by a live attenuated tetravalent dengue vaccine are directed against highly conserved epitopes.

Journal of virology

Weiskopf D,Angelo MA,Bangs DJ,Sidney J,Paul S,Peters B,de Silva AD,Lindow JC,Diehl SA,Whitehead S,Durbin A,Kirkpatrick B,Sette A

48-0458 was used in Flow cytometry/Cell sorting to characterise the CD8(+) T cell responses after live attenuated dengue vaccination.

Thu Jan 01 00:00:00 EST 2015

Automatic Generation of Validated Specific Epitope Sets.

Journal of immunology research

Carrasco Pro S,Sidney J,Paul S,Lindestam Arlehamn C,Weiskopf D,Peters B,Sette A

48-0458 was used in Flow cytometry/Cell sorting to develop a method to automatically select reference epitope sets according to a categorization system employed by the IEDB.

Mon Sep 26 00:00:00 EDT 2016

Transcriptional profile of tuberculosis antigen-specific T cells reveals novel multifunctional features.

Journal of immunology (Baltimore, Md. : 1950)

Arlehamn CL,Seumois G,Gerasimova A,Huang C,Fu Z,Yue X,Sette A,Vijayanand P,Peters B

48-0458 was used in Flow cytometry/Cell sorting to study the role of CD4(+) T cells in latent tuberculosis infection, showing that tuberculosis antigen-specific T cells have novel multifunctional features.

Mon Sep 15 00:00:00 EDT 2014

Memory T cells in latent Mycobacterium tuberculosis infection are directed against three antigenic islands and largely contained in a CXCR3+CCR6+ Th1 subset.

PLoS pathogens

Lindestam Arlehamn CS,Gerasimova A,Mele F,Henderson R,Swann J,Greenbaum JA,Kim Y,Sidney J,James EA,Taplitz R,McKinney DM,Kwok WW,Grey H,Sallusto F,Peters B,Sette A

48-0458 was used in Flow cytometry/Cell sorting to further define the immunological footprint of Mycobacterium tuberculosis CD4 T cell recognition.

Tue Jan 01 00:00:00 EST 2013

Type 1 diabetes-associated IL2RA variation lowers IL-2 signaling and contributes to diminished CD4+CD25+ regulatory T cell function.

Journal of immunology (Baltimore, Md. : 1950)

Garg G,Tyler JR,Yang JH,Cutler AJ,Downes K,Pekalski M,Bell GL,Nutland S,Peakman M,Todd JA,Wicker LS,Tree TI

48-0458 was used in Flow cytometry/Cell sorting to explore IL2RA gene polymorphisms in Treg dysfunction and associations with autoimmune and inflammatory diseases.

Tue May 01 00:00:00 EDT 2012

Vaccine

Zhou X,McElhaney JE

48-0458 was used in Flow cytometry/Cell sorting to suggest that the cytolytic activity of CD4+ T cells responding to influenza is preserved with ageing, while that of effector memory and effector CD8+ T cells becomes diminished.

Thu Mar 03 00:00:00 EST 2011

Bioinformatics

Protein Aliases: CD45; CD45 antigen; L-CA; Leukocyte common antigen; Lyt-4; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Receptor-type tyrosine-protein phosphatase C; T100; T200; T200 glycoprotein; T200 leukocyte common antigen

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Gene Aliases: B220; CD45; CD45R; GP180; L-CA; LCA; LY5; PTPRC; T200

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UniProt ID: (Human) P08575

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Entrez Gene ID: (Human) 5788

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Function(s)

protein tyrosine phosphatase activity transmembrane receptor protein tyrosine phosphatase activity protein binding protein kinase binding protein phosphatase protein modifying enzyme

Process(es)

negative regulation of T cell mediated cytotoxicity negative regulation of cytokine-mediated signaling pathway hematopoietic progenitor cell differentiation immunoglobulin biosynthetic process negative regulation of protein kinase activity protein dephosphorylation negative regulation of cell adhesion involved in substrate-bound cell migration cell surface receptor signaling pathway dephosphorylation T cell differentiation positive regulation of B cell proliferation peptidyl-tyrosine dephosphorylation B cell proliferation positive regulation of T cell proliferation cell cycle phase transition positive regulation of protein kinase activity bone marrow development stem cell development T cell receptor signaling pathway B cell receptor signaling pathway positive regulation of antigen receptor-mediated signaling pathway release of sequestered calcium ion into cytosol defense response to virus regulation of cell cycle positive regulation of hematopoietic stem cell migration positive regulation of stem cell proliferation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD45RA Monoclonal Antibody (HI100), eFluor™ 450, eBioscience™

CD45RA Antibody (48-0458-42) in Flow

Product Details

48-0458-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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