Flow cytometry protocols handbook
Protocols that fit your needs in flow cytometry ranging from sample preparation to numerous cell stimulation conditions, staining, immunophenotyping, and data analysis strategies.
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| 3 | 488/532 | 555/20 | 553 | 568 | (in buffer): 4 (in antifade): 4 | microscopy, flow cytometry, fluorescence microplate reader |
Probes with high fluorescence quantum yield and high photostability allow detection of low-abundance biological structures with great sensitivity. Invitrogen Alexa Fluor 555 dye molecules can be attached to proteins at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection.
We offer Alexa Fluor 555 dye, conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling and detection. In addition, reactive dye forms and protein labeling kits are available to allow you to generate your own antibody conjugates or probes.
Search Alexa Fluor 555 secondary antibodies Protein Labeling Reagents Selection Guide (NHS ester, maleimide, etc.)
Additional products
Figure 1.A muntjac skin fibroblast was fixed and permeabilized. Mitochondria were labeled with anti–OxPhos Complex V inhibitor protein mouse IgG1 and visualized using orange-fluorescent Alexa Fluor 555 goat anti–mouse IgG. F-actin was labeled with green-fluorescent Alexa Fluor 488 phalloidin, and the nucleus was stained with TO-PRO 3 iodide (pseudocolored magenta).
Figure 2.HeLa cells labeled using NucBlue Live ReadyProbes Reagent and Alexa Fluor Plus 555 Phalloidin show superb multiplexing capability and staining specificity. Cells were mounted in ProLong Glass Antifade Mountant. Images were generated using an EVOS M7000 Imaging System with an Olympus 60X Apochromat Oil objective using DAPI and RFP EVOS light cubes.
Additional resources
Fluorophore and reagent selection guide for flow cytometry
A handy reference poster featuring the broad range of our available dyes and labeling reagents.
Imaging protocol handbook
Protocols that fit your needs in imaging ranging from sample and assay preparation to staining, labeling, and data analysis strategies.
Fluorophore and reagent selection guide for cell imaging
A tool for selecting the optimal fluorescent dyes for your Invitrogen EVOS cell imaging systems.
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Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Brilliant Ultra Violet™ and Brilliant Violet™ are trademarks or registered trademarks of Becton, Dickinson and Company or its affiliates, and are used under license.
For Research Use Only. Not for use in diagnostic procedures.