Flow cytometry protocols handbook
Protocols that fit your needs in flow cytometry ranging from sample preparation to numerous cell stimulation conditions, staining, immunophenotyping, and data analysis strategies.
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R-phycoerythrin (R-PE) is an intensely bright phycobiliprotein isolated from red algae that exhibits extremely bright red-orange fluorescence with high quantum yields. In practical applications, the sensitivity of R-PE conjugates is usually 5 to 10 times greater than those of the corresponding fluorescein conjugates. In addition, R-PE exhibits:
- Long-wavelength fluorescence emission
- Large Stokes shifts
- Quench resistance due to the protein backbone
- Very high-water solubility
- Homogeneous structure with defined molecular weights
R-PE is a large molecule used for fluorescence-based detection, primarily in flow cytometry, microarray assays, ELISAs, and other applications that require high sensitivity, but not photostability. We offer a broad range of R-PE products, including primary antibodies, secondary antibodies, streptavidin, and tandem conjugates.
Search R-PE primary antibodies Search R-PE secondary antibodies
Figure 1. Flow cytometry histogram showing human mononuclear cells stained with anti-CD4 complexed with Invitrogen R‑phycoerythrin Zenon labeling reagent.
Figure 2. Flow cytometry dot plots showing CD4 APC positive cells. Normal human peripheral blood cells were unstimulated (left) or stimulated (right) with Cell Stimulation Cocktail (plus protein transport inhibitors). Cells were intracellularly stained with Anti-Human CD4 APC and Rat IgG2a K Isotype Control PE or Anti-Human IL-2 PE. Viable cells, as determined by Fixable Viability Dye eFluor 780, in the lymphocyte gate were used for analysis.
Additional resources
Fluorophore and reagent selection guide for flow cytometry
A handy reference poster featuring the broad range of our available dyes and labeling reagents.
Imaging protocol handbook
Protocols that fit your needs in imaging ranging from sample and assay preparation to staining, labeling, and data analysis strategies.
Fluorophore and reagent selection guide for cell imaging
A tool for selecting the optimal fluorescent dyes for your Invitrogen EVOS cell imaging systems.
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Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Brilliant Ultra Violet™ and Brilliant Violet™ are trademarks or registered trademarks of Becton, Dickinson and Company or its affiliates, and are used under license.
For Research Use Only. Not for use in diagnostic procedures.