Staining performance compared to PerCP-Cyanine5.5. Direct comparison of mouse splenocytes stained with anti-mouse CD4 (clone RM4-5) conjugated to either PerCP-eFluor 710 dye (red line) or PerCP-Cyanine5.5 (pink line).
The PerCP-eFluor 710 tandem dye was developed as an optimal choice for the FL3 channel on a blue laser line. It is consistently two to three times brighter than PerCP-Cyanine5.5 when evaluated in clone-to-clone comparison tests on identical samples. Although PerCP-eFluor 710 is slightly red-shifted in its emission compared to PerCP-Cyanine5.5 (710 nm vs. 685 nm), it uses the same filter sets.
Benefits of PerCP-eFluor 710 include
- Greater mean fluorescence intensity (MFI) as compared to PerCP-Cyanine5.5
- Minimizes the need for compensation out of the PE detector
- Stable performance when treated with formaldehyde fixation or exposed to ambient light
PerCP-eFluor 710 dye dashboard
 Initial brightness | The blue laser–excitable PerCP-eFluor 710 tandem dye is designed for use in flow cytometry applications. No change to your instrumentation is needed if you already use PE-Cy5.5 or PerCP-Cy5.5, allowing you to easily add PerCP-eFluor 710 to your multicolor panels. | ||
 Photostability in buffer |
| 488 | 710/50 | 633 | 710 | ||
 Laser line |  Common filter set |  Excitation max |  Emission max |
Effects of light exposure or fixation on PerCP-eFluor 710 dye. Human PBMCs were stained with Anti-CD4 PerCP-eFluor 710 dye (clone SK3) and analyzed immediately (red line). The sample was thendivided and either exposed to ambient light for six hours (blue line) or fixed in 2% formaldehyde overnight at 4°C (green line) and analyzed again.
PerCP-eFluor 710 dye products
We offer PerCP-eFluor 710 dye conjugated to primary antibodies for use in flow cytometry.
Learn more here:
See all PerCP-eFluor 710 Primary Antibodies
See all PerCP-eFluor 710 Secondary Antibodies