Flow cytometry protocols handbook
Protocols that fit your needs in flow cytometry ranging from sample preparation to numerous cell stimulation conditions, staining, immunophenotyping, and data analysis strategies.
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| 4 | 355 | 670/25, 630 LP | 349 | 659 | (in buffer) 3 | flow cytometry |
Invitrogen Brilliant Ultra Violet™ 661 dye (BUV661) is a tandem dye excited by the 349 nm UV laser and emits at 659 nm. This dye has a medium-to-high relative brightness and can be used for cell surface, intracellular, and nuclear staining. BUV661 may have some cross-laser excitation with the red laser.
When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye- conjugated antibodies in a staining panel, we recommend using Super Bright Complete Staining Buffer or Brilliant Stain Buffer to minimize any non-specific polymer interactions.
We offer BUV661 dye conjugated to primary antibodies for use in flow cytometry.
Additional products
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Spectral signature of Brilliant Ultra Violet™ 661 dye. Data acquired on a 5-laser Cytek Aurora and normal human peripheral blood cells stained with CD3 Monoclonal Antibody (SK7), Brilliant Ultra Violet™ 661 were used for analysis.
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Intracellular staining of mouse splenocytes using Brilliant Ultra Violet™ 661. C57BL/6 mouse splenocytes were unstimulated (left) or stimulated for 48 hours with CD3e Monoclonal Antibody, Functional Grade (right). Cells were then surface-stained with CD45R (B220) Monoclonal Antibody, FITC and stained intracellularly, using the Foxp3/Transcription Factor Staining Buffer Set and protocol, with 1.0 µg of Ki-67 Monoclonal Antibody, Brilliant Ultra Violet™ 661. Viable cells in the lymphocyte gate were used for analysis, as determined by LIVE/DEAD™ Fixable Blue Dead Cell Stain Kit, for UV excitation
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Cell surface staining of C57BL/6 mouse splenocytes using Brilliant Ultra Violet™ 661. C57BL/6 mouse splenocytes were stained with CD3e Monoclonal Antibody, FITC and 0.25 µg of Rat IgG2a kappa Isotype Control, Brilliant Ultra Violet™ 661 dye (left) or 0.25 µg of CD4 Monoclonal Antibody, Brilliant Ultra Violet™ 661 dye (right). Viable cells in the lymphocyte gate were used for analysis, as determined by 7-AAD Viability Staining Solution.
Brilliant Ultra Violet dye background
Brilliant Ultra Violet dyes are a polymer dye-based technology and compatible with both spectral flow cytometry as well as traditional flow cytometry. The UV laser has unique channels far from heavily occupied detectors, allowing for larger panels.
Additional resources
Fluorophore and reagent selection guide for flow cytometry
A handy reference poster featuring the broad range of our available dyes and labeling reagents.
Imaging protocol handbook
Protocols that fit your needs in imaging ranging from sample and assay preparation to staining, labeling, and data analysis strategies.
Fluorophore and reagent selection guide for cell imaging
A tool for selecting the optimal fluorescent dyes for your Invitrogen EVOS cell imaging systems.
Cy™ is a trademark or registered trademark of GE Healthcare.
Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Brilliant Ultra Violet™ and Brilliant Violet™ are trademarks or registered trademarks of Becton, Dickinson and Company or its affiliates, and are used under license.
For Research Use Only. Not for use in diagnostic procedures.