Flow cytometry protocols handbook
Protocols that fit your needs in flow cytometry ranging from sample preparation to numerous cell stimulation conditions, staining, immunophenotyping, and data analysis strategies.
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| 2 | 633 | 780/60 | 756 | 785 | (in buffer) 1 | flow cytometry |
Invitrogen APC-eFluor 780 dye is a near-infrared fluorescent dye designed to be a high-performance tandem fluorophore for multicolor flow cytometry. It is designed for use with flow cytometers equipped with a red laser and have an emission peak of 780 nm. With similar spectral characteristics to APC-Alexa Fluor 750, APC-eFluor 780 requires no changes to your instrument configuration for use, allowing you to easily add APC-eFluor 780 to your multicolor panels.
We offer APC-eFluor 780 dye conjugated to primary antibodies for use in flow cytometry.
Additional products
- Equal or better sensitivity than APC-H7 tandem conjugates
- Stable performance when treated with formaldehyde fixation or exposed to ambient light
- Uses same filter sets as existing comparative fluorochromes
Comparison of APC-eFluor 780 dye to APC-H7 dye using flow cytometry. Mouse splenocytes were stained with CD8a Monoclonal Antibody (53-6.7), APC-eFluor 780 (blue line) or CD8a monoclonal antibody conjugated to APC-H7 (red line).
Stability analysis of APC-eFluor 780. Human peripheral blood mononuclear cells (PBMCs) were stained with CD8a Monoclonal Antibody (RPA-T8), APC-eFluor 780 and analyzed immediately (red line) on flow cytometer. The sample was then divided and either exposed to ambient light for six hours (blue line) or fixed in 2% formaldehyde for 30 minutes at room temperature (green line) and analyzed again.
Additional resources
Fluorophore and reagent selection guide for flow cytometry
A handy reference poster featuring the broad range of our available dyes and labeling reagents.
Imaging protocol handbook
Protocols that fit your needs in imaging ranging from sample and assay preparation to staining, labeling, and data analysis strategies.
Fluorophore and reagent selection guide for cell imaging
A tool for selecting the optimal fluorescent dyes for your Invitrogen EVOS cell imaging systems.
Cy™ is a trademark or registered trademark of GE Healthcare.
Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Brilliant Ultra Violet™ and Brilliant Violet™ are trademarks or registered trademarks of Becton, Dickinson and Company or its affiliates, and are used under license.
For Research Use Only. Not for use in diagnostic procedures.