| Restriction Enzyme Name | Catalog # | Package size | FastDigest Name | Catalog # | Package size | Sequence |
|---|---|---|---|---|---|---|
| AluI | ER0011 ER0012 | 600 u 3000 u | FastDigest AluI | FD0014 | 100 rxns | AG^CT TC^GA |
| Alw21I (BsiHKAI) | ER0021 | 500 u | FastDigest Alw21I | FD0024 | 100 rxns | GWGCW^C C^WCGWG |
| Alw26I | ER0031 | 1000 u | FastDigest Alw26I | FD0034 | 100 rxns | GTCTCN^NNNN CAGAGNNNNN^ |
| Alw44I | ER0041 | 1000 u | FastDigest Alw44I | FD0044 | 200 rxns | G^TGCAC CACGT^G |
| BamHI | ER0051 ER0052 ER0053 ER0055 | 10000 u 20000 u 5 x 4000 u 4000 u | FastDigest BamHI | FD0054 FD0055 | 800 rxns 2500 rxns | G^GATCC CCTAG^G |
| BcnI (NciI) | ER0061 | 1000 u | FastDigest BcnI | FD0064 | 200 rxns | CC^SGG GGS^CC |
| BglI | ER0071 ER0072 | 5 x 2000 u 2000 u | FastDigest BglI | FD0074 | 200 rxns | GCCNNNN^NGGC CGGN^NNNNCCG |
| BglII | ER0081 ER0082 | 500 u 2500 u | FastDigest BglII | FD0083 FD0084 | 100 rxns 200 rxns | A^GATCT TCTAG^A |
| Bpu1102I (BlpI) | ER0091 ER0092 | 200 u 1000 u | FastDigest Bpu1102I | FD0094 | 50 rxns | GC^TNAGC CGANT^CG |
| Bsp68I (NruI) | ER0111 | 800 u | FastDigest RruI | FD2154 | 100 rxns | TCG^CGA AGC^GCT |
| Bsp119I (BstBI) | ER0121 | 2500 u | FastDigest Bsp119I | FD0124 | 200 rxns | TT^CGAA AAGC^TT |
| Bsp120I (PspOMI) | ER0131 | 1500 u | FastDigest Bsp120I | FD0134 | 200 rxns | G^GGCCC CCCGG^G |
| Bsu15I (ClaI) | ER0141 ER0142 ER0145 | 1000 u 3000 u 600 u | FastDigest Bsu15I | FD0143 FD0144 | 50 rxns 100 rxns | AT^CGAT TAGC^TA |
| BsuRI (HaeIII) | ER0151 | 3000 u | FastDigest BsuRI | FD0154 | 400 rxns | GG^CC CC^GG |
| Cfr10I (BsrFI) | ER0181 | 200 u | FastDigest Cfr10I | FD0184 | 50 rxns | R^CCGGY YGGCC^R |
| Cfr13I (Sau96I) | ER0191 | 1000 u | FastDigest Cfr13I | FD0194 | 100 rxns | G^GNCC CCNG^G |
| Csp6I (CviQI) | ER0211 | 1500 u | FastDigest Csp6I | FD0214 | 100 rxns | G^TAC CAT^G |
| DraI | ER0221 ER0223 | 7500 u 1500 u | FastDigest DraI | FD0224 | 200 rxns | TTT^AAA AAA^TTT |
| Eam1104I (EarI) | ER0231 ER0232 | 300 u 1500 u | FastDigest Eam1104I | FD0234 | 50 rxns | CTCTTCN^NNN GAGAAGNNNN^ |
| Eam1105I (AhdI) | ER0241 | 1000 u | FastDigest Eam1105I | FD0244 | 100 rxns | GACNNN^NNGTC CTGNN^NNNCAG |
| Ecl136II (EcoICRI) | ER0251 | 1500 u | FastDigest Ecl136II | FD0254 | 100 rxns | GAG^CTC CTC^GAG |
| EcoO109I (DraII) | ER0261 | 2000 u | FastDigest EcoO109I | FD0264 | 200 rxns | RG^GNCCY YCCNG^GR |
| EcoRI | ER0271 ER0272 ER0273 ER0275 | 5000 u 5 x 5000 u HC, 25000 u 10000 u | FastDigest EcoRI | FD0274 FD0275 | 2500 rxns 800 rxns | G^AATTC CTTAA^G |
| Eco31I (BsaI) | ER0291 ER0292 | 1000 u 5000 u | FastDigest Eco31I | FD0293 FD0294 | 50 rxns 100 rxns | GGTCTCN^NNNN CCAGAGNNNNN^ |
| Eco32I (EcoRV) | ER0301 ER0302 ER0303 ER0305 | 4000 u HC, 10000 u 5 x 2000 u 2000 u | FastDigest Eco32I | FD0303 FD0304 | 200 rxns 400 rxns | GAT^ATC CTA^TAG |
| Eco47I (AvaII) | ER0311 ER0312 | 800 u 4000 u | FastDigest Eco47I | FD0314 | 200 rxns | G^GWCC CCWG^G |
| Eco47III (AfeI) | ER0321 ER0322 | 200 u 1000 u | FastDigest Eco47III | FD0324 | 20 rxns | AGC^GCT TCG^CGA |
| Eco52I (EagI) | ER0331 ER0332 | 2500 u 500 u | FastDigest H137Eco52I | FD0334 | 50 rxns | C^GGCCG GCCGG^C |
| Eco57I (AcuI) | ER0341 ER0342 | 200 u 1000 u | FastDigest Eco57I | FD0344 | 20 rxns | CTGAAGNNNNNNNNNNNNNNNN^ GACTTCNNNNNNNNNNNNNN^NN |
| Eco72I (PmlI) | ER0361 | 2000 u | FastDigest Eco72I | FD0364 | 200 rxns | CAC^GTG GTG^CAC |
| Eco81I (Bsu36I) | ER0371 ER0372 | 2500 u 500 u | FastDigest Eco81I | FD0374 | 50 rxns | CC^TNAGG GGANT^CC |
| Eco88I (AvaI) | ER0381 | 1000 u | FastDigest Eco88I | FD0384 | 200 rxns | C^YCGRG GRGCY^C |
| Eco91I (BstEII) | ER0391 ER0392 | 1000 u 5000 u | FastDigest Eco91I | FD0394 | 200 rxns | G^GTNACC CCANTG^G |
| Eco105I (SnaBI) | ER0401 ER0402 | 3000 u 600 u | FastDigest Eco105I | FD0404 | 50 rxns | TAC^GTA ATG^CAT |
| Eco130I (StyI) | ER0411 | 2500 u | FastDigest Eco130I | FD0414 | 200 rxns | C^CWWGG GGWWC^C |
| Eco147I (StuI) | ER0421 ER0422 | 1000 u 5000 u | FastDigest Eco147I | FD0424 | 100 rxns | AGG^CCT TCC^GGA |
| ScaI | ER0431 ER0432 | 5000 u 1000 u | FastDigest ScaI | FD0434 | 100 rxns | AGT^ACT TCA^TGA |
| EheI (SfoI) | ER0441 | 500 u | FastDigest EheI | FD0443 FD0444 | 20 rxns 50 rxns | GGC^GCC CCG^CGG |
| Esp3I (BsmBI) | ER0451 ER0452 | 200 u 1000 u | FastDigest Esp3I | FD0454 | 20 rxns | CGTCTCN^NNNN GCAGAGNNNNN^ |
| GsuI (BpmI) | ER0461 ER0462 | 100 u 500 u | FastDigest GsuI | FD0464 | 20 rxns | CTGGAGNNNNNNNNNNNNNNNN^ GACCTCNNNNNNNNNNNNNN^NN |
| Hin1I (BsaHI) | ER0471 | 300 u | FastDigest Hin1I | FD0474 | 100 rxns | GR^CGYC CYGC^RG |
| Hin6I (HinP1I) | ER0481 | 2000 u | FastDigest Hin6I | FD0484 | 200 rxns | G^CGC CGC^G |
| HincII (HindlI) | ER0491 ER0492 | 500 u 2500 u | FastDigest HincII | FD0494 | 100 rxns | GTY^RAC CAR^YTG |
| HindIII | ER0501 ER0502 ER0503 ER0505 | 10000 u HC, 25,000 u 5 x 5000 u 5000 u | FastDigest HindIII | FD0504 FD0505 | 2500 rxns 800 rxns | A^AGCTT TTCGA^A |
| HpaII | ER0511 ER0512 | 1000 u 5000 u | FastDigest HpaII | FD0514 | 200 rxns | C^CGG GGC^C |
| KpnI | ER0521 ER0522 ER0523 | HC, 20,000 u 5 x 4000 u 4000 u | FastDigest KpnI | FD0524 | 300 rxns | GGTAC^C C^CATGG |
| Kpn2I (BspEI) | ER0531 ER0532 | 500 u 2500 u | FastDigest Kpn2I | FD0534 | 100 rxns | T^CCGGA AGGCC^T |
| MspI (HpaII) | ER0541 ER0542 | 3000 u 5 x 3000 u | FastDigest MspI | FD0544 | 400 rxns | C^CGG GGC^C |
| MvaI (BstNI) | ER0551 | 2000 u | FastDigest MvaI | FD0554 | 200 rxns | CC^WGG GGW^CC |
| MluI | ER0561 ER0562 | 1000 u 5000 u | FastDigest MluI | FD0564 | 100 rxns | A^CGCGT TGCGC^A |
| NcoI | ER0571 ER0572 ER0575 | 1000 u 2500 u 500 u | FastDigest NcoI | FD0573 FD0574 FD0575 | 20 rxns 100 rxns 300 rxns | C^CATGG GGTAC^C |
| NdeI | ER0581 ER0582 ER0585 | 500 u 2500 u 4000 u | FastDigest NdeI | FD0583 FD0584 FD0585 | 2500 rxns 300 rxns 100 rxns | CA^TATG GTAT^AC |
| NotI | ER0591 ER0592 ER0593 ER0595 | 500 u HC, 1500 u 1500 u 300 u | FastDigest NotI | FD0593 FD0594 FD0595 FD0596 | 20 rxns 50 rxns 150 rxns 250 rxns | GC^GGCCGC CGCCGG^CG |
| PaeI (SphI) | ER0601 ER0602 | 500 u 2500 u | FastDigest PaeI | FD0604 | 20 rxns | GCATG^C C^GTACG |
| PstI | ER0611 ER0612 ER0615 | 10000 u 5 x 3000 u 3000 u | FastDigest PstI | FD0614 FD0615 | 2500 rxns 800 rxns | CTGCA^G G^ACGTC |
| PvuI | ER0621 ER0622 | 300 u 1500 u | FastDigest PvuI | FD0624 | 20 rxns | CGAT^CG GC^TAGC |
| PvuII | ER0631 ER0633 ER0635 | 2500 u HC, 12500 u 5000 u | FastDigest PvuII | FD0634 | 400 rxns | CAG^CTG GTC^GAC |
| SalI | ER0641 ER0642 ER0645 | 2000 u 5 x 1500 u 1500 u | FastDigest SalI | FD0644 | 200 rxns | G^TCGAC CAGCT^G |
| SduI (Bsp1286I) | ER0651 | 500 u | FastDigest SduI | FD0654 | 50 rxns | GDGCH^C C^HCGDG |
| SmaI | ER0661 ER0662 ER0663 ER0665 | 2000 u HC, 6000 u 5 x 1200 u 1200 u | FastDigest SmaI | FD0663 FD0664 | 200 rxns 100 rxns | CCC^GGG GGG^CCC |
| TaqI | ER0671 ER0672 | 3000 u 5 x 3000 u | FastDigest TaqI | FD0674 | 400 rxns | T^CGA AGC^T |
| XbaI | ER0681 ER0682 ER0683 ER0685 | 3000 u HC, 7500 u 5 x 1500 u 1500 u | FastDigest XbaI | FD0684 FD0685 | 300 rxns 750 rxns | T^CTAGA AGATC^T |
| XhoI | ER0691 ER0692 ER0693 ER0695 | 5000 u HC, 10000 u 5 x 2000 u 2000 u | FastDigest XhoI | FD0694 FD0695 | 400 rxns 1200 rxns | C^TCGAG GAGCT^C |
| Bst1107I (BstZ17I) | ER0701 | 500 u | FastDigest Bst1107I | FD0704 | 100 rxns | GTA^TAC CAT^ATG |
| Van91I (PflMI) | ER0711 ER0712 | 400 u 2000 u | FastDigest Van91I | FD0714 | 100 rxns | CCANNNN^NTGG GGTN^NNNNACC |
| BclI | ER0721 ER0722 ER0725 | 3000 u 5000 u 1000 u | FastDigest BclI | FD0724 | 300 rxns | T^GATCA ACTAG^T |
| Mph1103I (NsiI) | ER0731 ER0732 | 1000 u 5000 u | FastDigest Mph1103I | FD0734 | 100 rxns | ATGCA^T T^ACGTA |
| CpoI (RsrII) | ER0741 ER0742 | 1000 u 200 u | FastDigest CpoI | FD0744 | 20 rxns | CG^GWCCG GCCWG^GC |
| MunI (MfeI) | ER0751 ER0752 | 300 u 1500 u | FastDigest MunI | FD0753 FD0754 | 20 rxns 50 rxns | C^AATTG GTTAA^C |
| Psp5II (PpuMI) | ER0761 | 500 u | FastDigest PpuMI (Psp5II) | FD0764 | 50 rxns | RG^GWCCY YCCWG^GR |
| SspI | ER0771 ER0772 | 500 u 2500 u | FastDigest SspI | FD0774 | 100 rxns | AAT^ATT TTA^TAA |
| Bsp143I (Sau3AI) | ER0781 ER0782 | 300 u 1500 u | FastDigest Bsp143I | FD0784 | 40 rxns | ^GATC CTAG^ |
| HinfI | ER0801 ER0802 ER0803 | HC, 10000 u 5 x 2000 u 2000 u | FastDigest HinfI | FD0804 | 400 rxns | G^ANTC CTNA^G |
| MboI | ER0811 ER0812 | 300 u 1500 u | FastDigest MboI | FD0814 | 50 rxns | ^GATC CTAG^ |
| MboII | ER0821 ER0822 | 300 u 1500 u | FastDigest MboII | FD0824 | 50 rxns | GAAGANNNNNNNN^ CTTCTNNNNNNN^N |
| BspTI (AflII) | ER0831 | 1000 u | FastDigest H81BspTI | FD0834 | 150 rxns | C^TTAAG GAATT^C |
| Pfl23II (BsiWI) | ER0851 | 300 u | FastDigest Pfl23II | FD0854 | 50 rxns | C^GTACG GCATG^C |
| BseGI (BtsCI) | ER0871 ER0872 | 500 u 2500 u | FastDigest BseGI | FD0874 | 100 rxns | GGATGNN^ CCTAC^NN |
| BseNI (BsrI) | ER0881 ER0882 | 5000 u 1000 u | FastDigest BseNI | FD0884 | 100 rxns | ACTGGN^ TGAC^CN |
| Bsh1285I (BsiEI) | ER0891 | 600 u | FastDigest Bsh1285I | FD0894 | 100 rxns | CGRY^CG GC^YRGC |
| Acc65I (Asp718I) | ER0901 ER0902 | 1000 u 5000 u | FastDigest Acc65I | FD0904 | 100 rxns | G^GTACC CCATG^G |
| VspI (AseI) | ER0911 ER0912 | 1000 u 5000 u | FastDigest VspI | FD0914 | 200 rxns | AT^TAAT TAAT^TA |
| Bsh1236I (BstUI) | ER0921 ER0922 | 500 u 2500 u | FastDigest Bsh1236I | FD0924 | 100 rxns | CG^CG GC^GC |
| Bsp1407I (BsrGI) | ER0931 ER0932 | 1500 u 300 u | FastDigest Bsp1407I | FD0933 FD0934 | 50 rxns 100 rxns | T^GTACA ACATG^T |
| Psp1406I (AclI) | ER0941 ER0942 | 300 u 1500 u | FastDigest AclI (Psp1406I) | FD0944 | 20 rxns | AA^CGTT TTGC^AA |
| Mva1269I (BsmI) | ER0961 ER0962 | 200 u 1000 u | FastDigest Mva1269I | FD0964 | 50 rxns | GAATGCN^ CTTAC^GN |
| NheI | ER0971 ER0972 ER0975 | 1000 u 2500 u 500 u | FastDigest NheI | FD0973 FD0974 | 50 rxns 100 rxns | G^CTAGC CGATC^G |
| Tru1I (MseI) | ER0981 ER0982 ER0983 | 300 u 1500 u HC, 1500 u | FastDigest Tru1I | FD0984 | 50 rxns | T^TAA AAT^T |
| AatII | ER0991 ER0992 | 1500 u 300 u | FastDigest AatII | FD0994 | 50 rxns | GACGT^C C^TGCAG |
| BshNI (BanI) | ER1001 | 2000 u | FastDigest BshNI | FD1004 | 300 rxns | G^GYRCC CCRYG^G |
| BpiI (BbsI) | ER1011 ER1012 | 200 u 1000 u | FastDigest BpiI | FD1014 | 20 rxns | GAAGACNN^NNNN CTTCTGNNNNNN^ |
| BstXI | ER1021 ER1022 | 2500 u 500 u | FastDigest BstXI | FD1024 | 100 rxns | CCANNNNN^NTGG GGTN^NNNNNACC |
| KspAI (HpaI) | ER1031 ER1032 | 500 u 2500 u | FastDigest H199KspAI | FD1034 | 50 rxns | GTT^AAC CA^TTG |
| MnlI | ER1071 ER1072 | 300 u 1500 u | FastDigest MnlI | FD1074 | 50 rxns | CCTCNNNNNNN^ GGAGNNNNNN^N |
| BseDI (BsaJI) | ER1081 ER1082 | 1500 u 300 u | FastDigest BseDI | FD1084 | 20 rxns | C^CNNGG GGNNC^C |
| PauI (BssHII) | ER1091 ER1092 | 200 u 1000 u | FastDigest PteI | FD2134 | 20 rxns | G^CGCGC CGCGC^G |
| RsaI | ER1121 ER1122 | 5000 u 1000 u | FastDigest RsaI | FD1124 | 100 rxns | GT^AC CA^TG |
| SacI | ER1131 ER1132 ER1135 | 1200 u 5 x 1200 u 2000 u | FastDigest SacI | FD1133 FD1134 | 100 rxns 200 rxns | GAGCT^C C^TCGAG |
| TaiI (MaeII) | ER1141 ER1142 | 2000 u 400 u | FastDigest TaiI | FD1144 | 50 rxns | ACGT^ ^TGCA |
| BspLI (NlaIV) | ER1151 ER1152 | 200 u 1000 u | FastDigest BspLI | FD1154 | 20 rxns | GGN^NCC CCN^NGG |
| BfmI (SfcI) | ER1161 ER1162 | 200 u 1000 u | FastDigest BfmI | FD1164 | 20 rxns | C^TRYAG GAYRT^C |
| Bpu10I | ER1181 | 200 u | FastDigest Bpu10I | FD1184 | 20 rxns | CC^TNAGC GGANT^CG |
| SdaI (SbfI) | ER1191 ER1192 | 300 u 1500 u | FastDigest SdaI | FD1194 | 20 rxns | CCTGCA^GG GG^ACGTCC |
| BseLI (BslI) | ER1201 | 500 u | FastDigest BseLI | FD1204 | 100 rxns | CCNNNNN^NNGG GGNN^NNNNNCC |
| MlsI (MscI) | ER1211 ER1212 | 1000 u 200 u | FastDigest MlsI | FD1214 | 50 rxns | TGG^CCA ACC^GGT |
| NsbI (FspI) | ER1221 | 400 u | FastDigest NsbI | FD1224 | 50 rxns | TGC^GCA ACG^CGT |
| AdeI (DraIII) | ER1231 | 500 u | FastDigest AdeI | FD1234 | 50 rxns | CACNNN^GTG GTG^NNNCAC |
| SmiI (SwaI) | ER1241 | 1000 u | FastDigest SmiI | FD1244 | 200 rxns | ATTT^AAAT TAAA^TTTA |
| BcuI (SpeI) | ER1251 ER1252 | 400 u 2000 u | FastDigest BcuI | FD1253 FD1254 | 20 rxns 50 rxns | A^CTAGT TGATC^A |
| BseMI (BsrDI) | ER1261 ER1262 | 500 u 100 u | FastDigest BseMI | FD1264 | 20 rxns | GCAATGNN^ CGTTAC^NN |
| MbiI (BsrBI) | ER1271 | 1000 u | FastDigest MbiI | FD1274 | 100 rxns | CCG^CTC GGC^GAG |
| PagI (BspHI) | ER1281 ER1282 | 400 u 2000 u | FastDigest PagI | FD1284 | 50 rxns | T^CATGA AGTAC^T |
| TatI | ER1291 ER1292 | 500 u 100 u | FastDigest TatI | FD1294 | 20 rxns | W^GTACW WCATG^W |
| XagI (EcoNI) | ER1301 | 1000 u | FastDigest XagI | FD1304 | 100 rxns | CCTNN^NNNAGG GGANNN^NNTCC |
| BplI | ER1311 ER1312 | 100 u 500 u | FastDigest BplI | FD1314 | 20 rxns | GAAGACNN^NNNN CTTCTGNNNNNN^ |
| PsyI (Tth111I) | ER1331 | 1000 u | FastDigest PsyI | FD1334 | 100 rxns | GACN^NNGTC CTGNN^NCAG |
| MssI (PmeI) | ER1341 ER1342 | 250 u 1250 u | FastDigest MssI | FD1344 | 100 rxns | GTTT^AAAC CAAA^TTTG |
| TasI (Tsp509I) | ER1351 ER1352 | 5000 u 1000 u | FastDigest TasI | FD1354 | 100 rxns | ^AATT TTAA^ |
| TaaI (HpyCH4III) | ER1361 ER1362 | 200 u 1000 u | FastDigest TaaI | FD1364 | 20 rxns | ACN^GT TG^NCA |
| SchI (MlyI) | ER1371 | 1000 u | FastDigest SchI | FD1374 | 100 rxns | GAGTCNNNNN^ CTCAGNNNNN |
| XapI (ApoI) | ER1381 | 500 u | FastDigest XapI | FD1383 FD1384 | 50 rxns 100 rxns | R^AATTY YTTAA^R |
| CaiI (AlwNI) | ER1391 | 500 u | FastDigest CaiI | FD1394 | 50 rxns | CAGNNN^CTG GTC^NNNGAC |
| BseMII (BspCNI) | ER1401 | 100 u | FastDigest H49BseMII | FD1404 | 20 rxns | GCAATGNN^ CGTTAC^NN |
| ApaI | ER1411 ER1415 | 3000 u 5000 u | FastDigest ApaI | FD1414 | 300 rxns | GGGCC^C C^CCGGG |
| Bme1390I (ScrFI) | ER1421 ER1422 | 2500 u 500 u | FastDigest H27Bme1390I | FD1424 | 100 rxns | CC^NGG GGN^CC |
| BoxI (PshAI) | ER1431 | 500 u | FastDigest BoxI | FD1434 | 100 rxns | GACNN^NNGTC CTGNN^NNCAG |
| BseSI (Bme1580I) | ER1441 | 500 u | FastDigest BseSI | FD1444 | 50 rxns | GKGCM^C C^MCGKG |
| BseXI (BbvI) | ER1451 ER1452 | 100 u 500 u | FastDigest BseXI | FD1454 | 20 rxns | GCAGCNNNNNNNN^NNNN CGTCGNNNNNNNNNNNN^ |
| BshTI (AgeI) | ER1461 ER1462 | 1000 u 200 u | FastDigest BshTI | FD1464 | 20 rxns | A^CCGGT TGGCC^A |
| XceI (NspI) | ER1471 ER1472 | 500 u 2500 u | FastDigest XceI | FD1474 | 50 rxns | RCATG^Y Y^GTACR |
| XmiI (AccI) | ER1481 ER1482 | 2000 u 400 u | FastDigest XmiI | FD1484 | 50 rxns | GT^M K AC CAK M^TG |
| NmuCI (Tsp45I) | ER1511 | 200 u | FastDigest NmuCI | FD1514 | 20 rxns | ^GTSAC CASTG^ |
| PdiI (NaeI) | ER1521 ER1522 | 1000 u 200 u | FastDigest PdiI | FD1524 | 50 rxns | GCC^GGC CGG^CCG |
| PdmI (XmnI) | ER1531 ER1532 | 500 u 2500 u | FastDigest PdmI | FD1534 | 100 rxns | GAANN^NNTTC CTTNN^NNAAG |
| PsuI (BstYI) | ER1551 | 500 u | FastDigest PsuI | FD1554 | 150 rxns | R^GATCY YCTAG^R |
| XmaJI (AvrII) | ER1561 ER1562 | 1000 u 200 u | FastDigest XmaJI | FD1564 | 20 rxns | C^CTAGG GGATC^C |
| Hpy8I (MjaIV) | ER1571 ER1572 | 200 u 1000 u | FastDigest Hpy8I | FD1574 | 20 rxns | GTN^NAC CAN^NTG |
| LweI (SfaNI) | ER1621 ER1622 | 100 u 500 u | FastDigest BmsI | FD2124 | 20 rxns | GCATCNNNNN^NNNN CGTAGNNNNNNNNN^ |
| OliI (AleI) | ER1631 ER1632 | 1000 u 200 u | FastDigest OliI | FD1634 | 20 rxns | CACNN^NNGTG GTGNNNNCAC |
| SatI (Fnu4HI) | ER1641 ER1642 | 200 u 1000 u | FastDigest SatI | FD1644 | 20 rxns | GC^NGC CGN^CG |
| TauI | ER1651 ER1652 | 50 u 250 u | FastDigest TauI | FD1654 | 20 rxns | GCSG^C C^GS CG |
| FspAI | ER1661 ER1662 | 500 u 100 u | FastDigest FspAI | FD1664 | 20 rxns | RTGC^GCAY YACGCGTR |
| DpnI | ER1701 ER1702 ER1705 | 1000 u 2500 u 500 u | FastDigest DpnI | FD1703 FD1704 | 100 rxns 50 rxns | GA^TC CT^AG |
| BseJI (BsaBI) | ER1711 | 2000 u | FastDigest BseJI | FD1714 | 200 rxns | GATNN^NNATC CTANN^NNTAG |
| AasI (DrdI) | ER1721 | 300 u | FastDigest AasI | FD1724 | 25 rxns | GACNNNN^NNGTC CTGNN^NNNNCAG |
| HpyF10VI (MwoI) | ER1731 ER1732 | 300 u 1500 u | FastDigest HpyF10VI | FD1734 | 20 rxns | GCNNNNN^NNGC CGNN^NNNNNCG |
| BveI (BspMI) | ER1741 | 250 u | FastDigest BveI | FD1744 | 50 rxns | ACCTGCNNNN^NNNN TGGACGNNNNNNNN^ |
| PfoI | ER1751 | 200 u | FastDigest PfoI | FD1754 | 20 rxns | T^CCNGGA AGGNCC^T |
| FspBI (BfaI) | ER1761 ER1762 | 500 u 2500 u | FastDigest H165FspBI | FD1764 | 50 rxns | C^TAG GAT^C |
| PfeI (TfiI) | ER1781 | 500 u | FastDigest PfeI | FD1784 | 50 rxns | G^AWTC CTWA^G |
| SsiI (AciI) | ER1791 | 200 u | FastDigest SsiI | FD1794 | 20 rxns | C^CGC GGC^G |
| FaqI (BsmFI) | ER1811 | 100 u | FastDigest FaqI | FD1814 | 20 rxns | GGGACNNNNNNNNNN^NNNN CCCTGNNNNNNNNNNNNNN^ |
| SfiI | ER1821 | 1000 u | FastDigest SfiI | FD1824 | 150 rxns | GGCCNNNN^NGGCC CCGGN^NNNNCCGG |
| Hin1II (NlaIII) | ER1831 | 300 u | FastDigest Hin1II | FD1834 | 100 rxns | CATG^ ^GTAC |
| HhaI | ER1851 | 2000 u | FastDigest HhaI | FD1854 | 200 rxns | GCG^C C^GCG |
| HpyF3I (DdeI) | ER1881 ER1882 | 500 u 2500 u | FastDigest HpyF3I | FD1884 | 50 rxns | C^TNAG GANT^C |
| SgsI (AscI) | ER1891 ER1892 | 300 u 1500 u | FastDigest SgsI | FD1894 | 100 rxns | GG^CGCGCC CCGCGC^GG |
| CseI (HgaI) | ER1901 | 100 u | FastDigest H107CseI | FD1904 | 20 rxns | GACGCNNNNN^NNNNN CTGCGNNNNNNNNNN^ |
| LguI (SapI) | ER1931 ER1932 | 100 u 500 u | FastDigest LguI | FD1934 | 20 rxns | GCTCTTCN^NNN CGAGAAGNNNN^ |
| AjuI | ER1951 | 100 u | FastDigest AjuI | FD1954 | 20 rxns | NNNNN^NNNNNNNGAANNNNNNNTTGGNNNNNNNNNNN^ ^NNNNNNNNNNNNCTTNNNNNNNAACCNNNNNN^NNNNN |
| Ppu21I (BsaAI) | ER1971 | 500 u | FastDigest BsaAI (Ppu21I) | FD1974 | 50 rxns | YAC^GTR RTG^CAY |
| RseI (MslI) | ER2001 ER2002 | 1000 u 200 u | FastDigest RseI | FD2004 | 20 rxns | CAYNN^NNRTG GTRNN^NNYAC |
| MreI (Sse232I) | ER2021 | 300 u | FastDigest MreI | FD2024 | 20 rxns | CG^CCGGCG GCGGCC^GC |
| BspOI (BmtI) | ER2041 | 200 u | FastDigest BspOI | FD2044 | 20 rxns | GCTAG^C C^GATCG |
| AanI (PsiI) | ER2061 | 200 u | FastDigest AanI | FD2064 | 20 rxns | TTA^TAA AAT^ATT |
| Lsp1109I (BbvI) | ER2071 | 200 u | FastDigest Lsp1109I | FD2074 | 100 rxns | GCAGCNNNNNNNN^NNNN CGTCGNNNNNNNNNNNN^ |
| MauBI | ER2081 | 100 u | FastDigest MauBI | FD2084 | 20 rxns | CG^CGCGCG GCGCGC^GC |
| SfaAI (AsiSI) | ER2091 | 1000 u | FastDigest SfaAI | FD2094 | 100 rxns | GCGAT^CGC CGC^TAGCG |
| TscaAI (TspRI) | ER2101 | 1000 u | FastDigest TscAI | FD2104 | 100 rxns | NNCASTGNN^ ^NNGTSACNN |
| PacI | ER2201 ER2202 | 250 u 1250 u | FastDigest PacI | FD2204 | 25 rxns | TTAAT^TAA AAT^TAATT |