Table 2.1 Molecular Probes thiol-reactive dyes excited with visible light.
Derivative | Abs * | Em * | Maleimide | Haloacetamide | Bromomethyl | Halide | Cystine C or Thiosulfate T |
---|---|---|---|---|---|---|---|
Alexa Fluor 488 | 495 | 519 | A10254 M | ||||
Alexa Fluor 532 | 532 | 553 | A10255 | ||||
Alexa Fluor 546 | 556 | 575 | A10258 M | ||||
Alexa Fluor 555 | 555 | 565 | A20346 | ||||
Alexa Fluor 568 | 578 | 603 | A20341 M | ||||
Alexa Fluor 594 | 590 | 617 | A10256 M | ||||
Alexa Fluor 633 | 632 | 647 | A20342 M | ||||
Alexa Fluor 647 | 650 | 665 | A20347 | ||||
Alexa Fluor 660 | 663 | 690 | A20343 | ||||
Alexa Fluor 680 | 679 | 702 | A20344 | ||||
Alexa Fluor 750 | 749 | 775 | A30459 | ||||
BODIPY FL | 505 | 513 | B10250 | D6003 | B20340 C T30453 T | ||
BODIPY TMR | 542 | 574 | B30466 | T30454 T | |||
BODIPY TR | 589 | 617 | T30455 T | ||||
BODIPY 493/503 | 493 | 503 | B2103 | ||||
BODIPY 499/508 | 499 | 508 | D20350 | ||||
BODIPY 507/545 | 508 | 543 | D6004 | ||||
BODIPY 577/618 | 577 | 618 | D20351 | ||||
BODIPY 630/650 | 625 | 640 | B22802 | T30456 T | |||
4-Dimethylamino phenylazophenyl | 419 | NA | D1521 | ||||
Eosin | 524 | 544 | E118 5 | ||||
Fluorescein | 494 | 518 | F150 5 | I30451 5 I30452 6 | B1355 5 | ||
Lucifer yellow | 426 | 531 | L1338 | ||||
NBD | 478 | 541 | I9 † D2004 | ||||
Oregon Green 488 | 496 | 524 | O6034 5 | O6010 M | |||
PyMPO | 415 | 570 | M6026 | ||||
QSY 7 | 560 | NA | Q10257 | ||||
QSY 9 | 562 | NA | Q30457 | ||||
QSY 35 | 475 | NA | Q20348 | ||||
Rhodamine Red | 570 | 590 | R6029 M | ||||
Sulfonerhodamine | 555 | 580 | B10621 § | ||||
Tetramethylrhodamine | 555 | 580 | T6027 5 T6028 6 | T6006 5 | |||
Texas Red | 595 | 615 | T6008 M | T6009 M | |||
* Absorption (Abs) and emission (Em) maxima, in nm. † Iodoacetate ester. ‡ Like the NBD probes, ABD-F (F6053) is a benz-2-oxa-1,3-diazole, except that it is sulfonated (i.e., an SBD probe) instead of nitrated (i.e., an NBD probe); its reaction product with dimethylaminoethanethiol has Abs/Em maxima of 376/510 nm. § Bifunctional crosslinker. 5 = 5-Isomer. 6 = 6-Isomer. M = Mixed isomers. C = BODIPY FL L-cystine. T = TS-Link fluorescent thiosulfate. NA = Not applicable. |
For Research Use Only. Not for use in diagnostic procedures.