Table 20.2 Summary of the pH response of our LysoSensor probes.
| Cat. No. | LysoSensor Probe | Abs/Em * (nm) | pKa † | Useful pH Range † |
|---|---|---|---|---|
| L7533 | LysoSensor Blue DND-167 | 373/425 | 5.1 | 4.5–6.0 |
| L7534 | LysoSensor Green DND-153 | 442/505 | 7.5 | 6.5–8.0 |
| L7535 | LysoSensor Green DND-189 | 443/505 | 5.2 | 4.5–6.0 |
| L7545 | LysoSensor Yellow/Blue DND-160 | 384/540 ‡ 329/440 § | 4.2 | 3.5–6.0 |
| * Absorption (Abs) and fluorescence emission (Em) maxima, at pH 5; values may vary somewhat in cellular environments. † All pKa values were determined in vitro; values are likely to be different in cells. ‡ At pH 3. § At pH 7. | ||||
For Research Use Only. Not for use in diagnostic procedures.