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- Long-Wavelength Rhodamines, Texas Red Dyes and QSY Quenchers—Section 1.6
- Fluorophores and Their Amine-Reactive Derivatives—Chapter 1
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Table 1.11 R0 values for QSY and dabcyl quenchers.
Donor |
Acceptor
| ||||
---|---|---|---|---|---|
QSY 35 | dabcyl | QSY 7 and QSY 9 | QSY 21 | ||
Alexa Fluor 350 | 47 | 50 | |||
Alexa Fluor 488 | 44 | 49 | 64 | ||
Alexa Fluor 546 | 25 | 29 | 67 | ||
Alexa Fluor 555 | 45 | ||||
Alexa Fluor 568 | 56 | 75 | |||
Alexa Fluor 594 | 77 | ||||
Alexa Fluor 647 | 69 | ||||
R0 values in angstroms (Å) represent the distance at which fluorescence resonance energy transfer from the donor dye to the acceptor dye is 50% efficient (Förster radius). Values were calculated from spectroscopic data as outlined (see Fluorescence Resonance Energy Transfer (FRET)—Note 1.2). |
For Research Use Only. Not for use in diagnostic procedures.