| 2020-07-27 | Extraction and Purification of Functional G Protein-Coupled Receptors from Expression Systems | Cell membrane protein extraction and isolation | GPCR Extraction and Stabilization Reagent |
| 2017-04-20 | ProcartaPlex Human Immuno-Oncology Checkpoint Panel: detection of soluble isoforms of immuno-oncology checkpoint markers | Multiplex Immunoassay | ProcartaPlex Human Immuno-Oncology Checkpoint Panel |
| 2016-09-20 | Characterization of antibody specificity using immunoprecipitation and mass spectrometry | Antibody Production, Mass Spectrometry, Immunoprecipitation | Antibodies |
| 2016-07-01 | Optimizing protein A purification of monoclonal antibodies when using the ExpiCHO Expression System. | Affinity Purification, Antibody Production | ExpiCHO Expression System |
| 2015-12-15 | Guide to gels and transfer methods for high molecular weight proteins, featuring the Pierce Power Blotter. | Electrophoresis and Staining, Western Blotting | Pierce Power Blotter |
| 2015-11-02 | Pierce Power Stainer: a fast, reliable device for Coomassie protein staining and destaining. | Electrophoresis and Staining, Western Blotting | Pierce Power Stainer |
| 2014-05-22 | Transfer EMSA gels using the Pierce G2 Fast Blotter. The Pierce G2 Fast Blotter provides rapid semi-dry transfer of DNA (TBE) gels to nylon membrane. | Electrophoresis and Staining, Western Blotting | Pierce G2 Fast Blotter – Power Stainer Upgrade |
| 2014-05-19 | An improved method for highly efficient isolation of primary mouse embryonic fibroblasts. Easily isolate and culture primary mouse embryonic fibroblasts (MEF). | Lysis and Extraction | Pierce Mouse Embryonic Fibroblast Isolation Kit |
| 2014-05-02 | Pierce qIP Assay: a novel, HTS-compatible protein-protein interaction assay system. A cell-based, quantitative co-immunoprecipitation method for protein interaction analysis involving a sensitive luciferase assay strategy. | Immunoprecipitation, Luciferase Assays | Pierce Agarose qIP Protein Interaction Kit, Tluc and HA Tags |
| 2014-04-02 | One-hour procedure to isolate primary cardiomyocytes from neonatal mouse and rat hearts. Easily isolate and culture highly viable, functional primary cardiomyocytes. | Lysis and Extraction | Pierce Primary Cardiomyocyte Isolation Kit |
| 2014-03-24 | Glycoprotein expression in a human IVT system. Expression of partially N-linked glycoproteins using the 1-Step Human Coupled in vitro Translation System. | Protein Expression | 1-Step Human Coupled IVT Kit - DNA |
| 2014-03-24 | Benchmarking experiments with Zeba Spin Desalting Columns. Protein recovery performance comparisons of Thermo Scientific Zeba Spin Desalting Columns to other commercially available desalting resins and columns. | Dialysis and Desalting | Zeba Micro Spin Desalting Columns, 7K MWCO, 75 µL |
| 2014-03-03 | Calculate the number of immobilized proteins per bead of agarose affinity supports. A consideration of relative sizes and dimensions of agarose resin beads, antibodies, proteins, chemical modification groups, and affinity ligands. | Affinity Purification | AminoLink Plus Coupling Resin |
| 2014-02-19 | Improved, all-in-one B-PER Reagent for bacterial protein extraction. B-PER Complete Reagent provides gentle, efficient, and cost effective protein extraction from E. coli and other bacterial cells. | Lysis and Extraction | B-PER Complete Bacterial Protein Extraction Reagent |
| 2014-01-28 | An efficient method for the isolation of highly functional primary neurons. Easily and reproducibly isolate and culture primary neurons. | Lysis and Extraction | Pierce Primary Neuron Isolation Kit |
| 2014-01-16 | Expression of highly active proteins using a cell-free human IVT system. Active caspase-3 produced by cell-free protein expression with the 1-Step Human High-Yield In Vitro Translation Kit is more functional than recombinant caspase-3 expressed in bacteria. | Protein Expression | 1-Step Human High-Yield Mini IVT Kit |
| 2014-01-10 | System for high-throughput, high-yield in vitro translation. HTP protein expression using 1-Step Human High-Yield IVT Kits and Pierce 96-well Microdialysis Plates. | Protein Expression | 1-Step Human High-Yield Mini IVT Kit |
| 2013-11-12 | Luciferase assays and BioTek Synergy microplate readers. Validation and comparison of single-step flash and dual-spectral luciferase reporter gene assays using the Synergy line of microplate readers. | Luciferase Assays | Pierce Renilla Luciferase Flash Assay Kit |
| 2013-10-22 | Choosing a vector and purification method for in vitro protein expression. Strategies for optimizing expression and purification of functional proteins produced using the Thermo Scientific 1-Step High Yield IVT protein expression system. | Protein Expression, Fusion Tag Methods | pT7CFE1-NHis Vector for Mammalian Cell-Free Protein Expression |
| 2013-09-11 | Analysis of androgen-dependent and -independent regulation of transcriptional activity. Monitor activity using a sensitive and optimized magnetic ChIP kit. | Immunoprecipitation, Molecular Biology | Pierce Magnetic ChIP Kit |
| 2013-08-29 | Efficient mammalian membrane protein extraction. Fast and simple enrichment of integral membrane proteins and membrane-associated proteins. | Lysis and Extraction | Mem-PER Plus Membrane Protein Extraction Kit |
| 2013-08-19 | Specific labeling, enrichment and quantitation of S-nitrosylated peptides using iodoTMT reagents. Measure protein and peptide cysteine modifications using multiplex quantitative mass spectrometry. | Mass Spectrometry, Post-translational Modification | iodoTMTsixplex Label Reagent Set, 1 x 0.2 mg |
| 2013-08-02 | Performance characterization of Pierce Glutathione Superflow Agarose. Purity, dynamic binding capacity, reusability and chemical compatibility properties of a glutathione resin for FPLC purification of GST tagged fusion proteins. | Fusion Tag Methods, Affinity Purification | Pierce Glutathione Superflow Agarose |
| 2013-07-11 | Performance characterization of HisPur Ni-NTA Superflow Agarose. Purity, dynamic binding capacity, reusability and chemical compatibility properties of a nickel-charged IMAC resin for FPLC purification of His-tagged proteins. | Fusion Tag Methods, Affinity Purification | HisPur Ni-NTA Superflow Agarose |
| 2013-06-26 | Improved Western blot detection using CCD digital imaging. Comparison of cooled-CCD digital imaging versus X-ray film for sensitivity, dynamic range and signal linearity with enhanced chemiluminescence (ECL). | Western Blotting, Electrophoresis and Staining | myECL Imager |
| 2013-06-24 | Versatile luciferases: new tools for reporter assays. Microplate luminometers equipped with reagent dispensers and optical filters provide optimal performance for luciferase assays with Cypridina, Gaussia, Renilla and firefly luciferase reporter genes. | Luciferase Assays | Pierce Gaussia Luciferase Flash Assay Kit |
| 2013-06-05 | Carrier protein activation and conjugation data for immunogen preparation. Experiments with KLH variants and other immunogens to validate solubility, maleimide activation consistency, peptide conjugation efficiency and immunogenicity. | Antibody Production | Imject mcKLH (in PBS) |
| 2013-06-04 | A versatile mass spectrometry sample preparation procedure for complex protein samples. Prepare high-quality peptide digests with high recovery from cultured cells. | Mass Spectrometry, Sample Preparation | Pierce Mass Spec Sample Prep Kit for Cultured Cells |
| 2013-05-22 | Protocol for in vitro protein expression using mRNA templates. Use the 1-Step Human Coupled DNA IVT kit to efficiently translate mRNA templates. | Protein Expression | 1-Step Human Coupled IVT Kit - DNA |
| 2013-05-02 | Performance characterization of HisPur Cobalt Superflow Agarose. Purity, dynamic binding capacity, reusability and chemical compatibility properties of a cobalt-charged IMAC resin. | Fusion Tag Methods, Affinity Purification | HisPur Cobalt Superflow Agarose |
| 2013-04-30 | Activation of the antioxidant response pathway by pesticide chemicals. Simultaneous measurement of the activation of ARE- and NF-κB-driven luciferase reporters by parkinsonian neurotoxins in neuronal cells. | Luciferase Assays | Pierce Gaussia Luciferase Flash Assay Kit |
| 2013-04-18 | Separation characteristics of dialysis membranes. Molecular weight cut-off (MWCO) specifications and rates of buffer exchange with Slide-A-Lyzer Dialysis Devices and Snakeskin Dialysis Tubing. | Dialysis and Desalting | Slide-A-Lyzer G2 Dialysis Cassettes, 10K MWCO, 3 mL |
| 2013-03-08 | Monitoring neuronal differentiation using multiplexed luciferase reporters. Characterization of early neuronal differentiation markers in precursor cell lines using multiplexed luciferase reporters and immunofluorescence imaging. | Luciferase Assays, IHC and Cell Imaging | Pierce Cypridina Luciferase Flash Assay Kit |
| 2013-03-07 | Chromatography cartridges for desalting and affinity purification. Data using the cartridge format for automated liquid chromatography (LC) systems. | Affinity Purification, Dialysis and Desalting | Zeba Micro Spin Desalting Columns, 7K MWCO, 75 µL |
| 2013-01-24 | Simultaneous dual-emission detection of luciferase reporter assays. Performing dual-spectral luciferase assays using the BMG Labtech POLARstar Omega plate reader. | Luciferase Assays, Protein Expression | Pierce Cypridina-Firefly Luciferase Dual Assay Kit |
| 2012-12-11 | Quantitative cancer stem cell phosphoprotein profiling. Using tandem mass labeling and LC-MS/MS to profile perturbations of an autocrine loop. | Mass Spectrometry, Post-translational Modification | Pierce Phosphoprotein Enrichment Chromatography Cartridges, 5mL |
| 2012-11-12 | Phosphoprotein enrichment from cell and tissue samples. Isolate phosphorylated proteins with an easy-to-use spin format | Post-translational Modification, Affinity Purification | Pierce Phosphoprotein Enrichment Chromatography Cartridges, 5mL |
| 2012-10-11 | Cell-free protein expression system for generating stable isotope-labeled proteins. Rapidly express protein standards for quantitative mass spectrometry. | Mass Spectrometry | 1-Step Heavy Protein IVT Kit |
| 2012-07-18 | Eliminate endotoxins from protein and antibody samples. A highly specific affinity resin enables endotoxin removal from various protein sources, sizes and charges. | Sample Preparation | Pierce High Capacity Endotoxin Removal Resin |
| 2012-07-09 | Removing endotoxins using a spin-column format. Fast and effective removal of endotoxins from protein samples. | Sample Preparation | Pierce High Capacity Endotoxin Removal Resin |
| 2012-06-13 | Luciferase assays in hard-to-transfect Jurkat cells. Obtain strong signals in cells with low transfection efficiency. | Luciferase Assays | Pierce Gaussia Luciferase Flash Assay Kit |
| 2012-05-18 | Fractionate subcellular proteins from tissue samples. Obtain proteins from five cellular components using a tissue-optimized kit. | Lysis and Extraction | Subcellular Protein Fractionation Kit for Tissues |
| 2012-05-10 | Method to isolate functional synaptosomes. Enrich neuronal synaptic proteins while maintaining phosphoprotein integrity. | Lysis and Extraction | Syn-PER Synaptic Protein Extraction Reagent |
| 2012-04-18 | Complete protein protection from proteases and phosphatases. Protease, phosphatase and combination inhibitor tablets provide a convenient format to protect proteins from degradation and dephosphorylation. | Lysis and Extraction, Sample Preparation | Pierce Protease Inhibitor Tablets, EDTA-free |
| 2012-03-05 | Optimized protein extraction reagent for neuronal tissue and primary cells. Obtain high yields of functional neuronal protein from all neuronal cell compartments. | Lysis and Extraction | N-PER Neuronal Protein Extraction Reagent |
| 2012-02-28 | Highly sensitive multiplex luciferase reporter assays. Monitor changes in NFkB and CRE promoter activity using bioluminescent reporter assays. | Luciferase Assays, Protein Expression | Pierce Cypridina-Firefly Luciferase Dual Assay Kit |
| 2012-02-24 | Using antibody-binding proteins for antibody purification. Choose a format based on the specific application and scale. | Antibody Purification, Affinity Purification | Pierce Protein A Plus Agarose |
| 2011-10-07 | Improved immobilization and conjugation of glycoproteins. Efficient aldehyde-hydrazide covalent coupling using a catalyst and an optimized buffer. | Immunoprecipitation, Protein Immobilization | GlycoLink IP Kit |
| 2011-06-01 | Western blotting using enhanced chemiluminescence. Choose the perfect substrate for your needs. | Western Blotting | Pierce ECL Western Blotting Substrate |
| 2011-06-01 | Serine hydrolase active-site probes for activity-based enzyme profiling. New fluorophosphonate probes selectively label and enrich active serine hydrolases. | Protein Labeling, Protein Assays | ActivX TAMRA-FP Serine Hydrolase Probe |
| 2011-06-01 | Selective enrichment of sialylated glycopeptides using TiO2 beads. Affinity purification of glycoproteins for mass spectrometry based on titanium dioxide beads (TiO2). | Mass Spectrometry | Pierce TiO2 Phosphopeptide Enrichment and Clean-up Kit |
| 2011-06-01 | Perform IP and co-IP experiments with high yield and low background. New Protein A/G magnetic beads enable manual and automated applications. | Immunoprecipitation, Affinity Purification | Pierce Protein A/G Magnetic Beads |
| 2011-06-01 | Optimize liquid chromatography with a peptide retention time calibration mixture. New mixture of 15 heavy peptides streamlines assay design for targeted peptide quantification. | Chromatography, Mass Spectrometry | Pierce Peptide Retention Time Calibration Mixture |
| 2011-06-01 | Calibration solutions for Thermo Scientific Mass Spectrometers. Ready-to-use standards enable quick instrument calibration. | Mass Spectrometry | Pierce LTQ ESI Positive Ion Calibration Solution |
| 2011-06-01 | New dimensions for low-volume sample dialysis. Self-contained devices give you the solution for high-protein recovery. | Dialysis and Desalting, Sample Preparation | Slide-A-Lyzer MINI Dialysis Device, 10K MWCO, 0.1 mL |
| 2011-06-01 | Introducing a Western blot substrate that is chemiluminescent and chemifluorescent. Detect your target by film, CCD cameras or laser-based imagers. | Western Blotting | Pierce ECL Plus Western Blotting Substrate |
| 2011-06-01 | Develop mid- to high-throughput SRM and MRM assays. New application-specific peptide libraries for quantitative mass spectrometry workflows. | Peptides, Mass Spectrometry | PEPotec SRM Custom Peptide Libraries |
| 2011-04-05 | Measure activation of small GTPases via their specific downstream effectors. Pull-down kits enrich active GTPases from cell or tissue lysates. | Protein Assays, Immunoprecipitation | Active Rac1 Pull-Down and Detection Kit |
| 2011-04-05 | GTPase enrichment using a new active-site probe. Introducing a desthiobiotin nucleotide probe for studying GTPases. | Protein Labeling, Affinity Purification | Pierce GTPase Enrichment Kit with GTP Probe |
| 2011-04-05 | Enhanced immunostaining means more signal and better sensitivity. Achieve better protein detection using a fraction of your valuable primary antibody. | IHC and Cell Imaging | Pierce Immunostain Enhancer |
| 2011-04-05 | Detection and localization of active GTPases in neuronal cell differentiation. GTPase pull-down kits enable powerful signal transduction studies. | IHC and Cell Imaging, Affinity Purification | Active Rac1 Pull-Down and Detection Kit |
