从这里开始学习或进一步提升您的蛋白质生物学知识
This modular, animated, and narrated eLearning course was developed to provide a succinct, contextual summary of common laboratory methods and techniques required to achieve optimal results in protein assays and experiments. There are knowledge checks throughout the course to test what you have learned.
视频演示了western blot流程中的每一个步骤，包括SDS-PAGE、转膜、封闭、使用抗体进行探针检测、化学发光检测及印迹剥离等步骤.
回顾质谱的发展历史、应用领域及方法论等，包括离子化（ESA，MALDI）、质量分析仪（TOF，四级杆，离子阱），离子检测器、MS/MS、LC-MS/MS及样品制备等内容.
该指南提供了如何选择适合底物，并根据底物选择起始的一抗和二抗稀释浓度所需的相关信息.
Pierce™蛋白方法文库是蛋白分析实验技术相关的百科文库。该文库会持续扩展及更新，无论是对实验新人，还是对经验丰富的研究人员来说都是非常有价值的资源.
Learn about methods and technologies for efficient cell lysis, protein extraction and fractionation, targeted inhibition of unwanted protease and phosphatase activity, and convenient devices and high performance resins for the purification and clean-up of proteins and antibodies for downstream applications.
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Learn about methods and technologies for separation and analysis of proteins by polyacrylamide gel electrophoresis (PAGE). Understand gel types (pouring, precast, gradient, 1D, 2D, etc.), gel and running buffer systems, sample preparation and loading, molecular weight markers (protein ladders), and gel stains.
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Learn about methods and technologies for western blot detection and imaging. Understand the reagents and instruments used for the efficient membrane transfer and detection of proteins, including wash and blocking buffers, secondary antibody conjugates, and chemiluminescent, colorimetric or fluorescent detection substrates.
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Learn about methods and technologies for identifying and measuring proteins. Understand protein quantitation reagents, ELISA and other immunoassays, protein interaction and activity assays, enzyme reporter assays, protein microarrays, and immunoprecipitation and pull-down tools.
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Learn about methods and technologies for peptide and protein analysis by high performance liquid chromatography and mass spectrometry (LC/MS). Understand sample prep tools, mass and isotope labeling strategies for quantitative proteomics, and the basics of MS data and analysis.
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Learn about methods and technologies for protein labeling, modification, and crosslinking. Understand the chemical functional groups and reaction chemistries required to crosslink and conjugate proteins to one another; to label proteins with biotin, PEG, or fluorescent dyes; or to reduce, cleave, and fragment proteins for use in assays.
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Learn about methods and technologies for preparing and using antibodies and recombinant proteins in protein analysis techniques. Understand how specific primary and secondary antibodies are produced, screened, purified, and modified to enable their use in a myriad of protein detection (identification and measurement) applications.
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查看蛋白质生物学学习中心内容——蛋白质试验方法文章、产品应用纪要、选择指南、网络讲座、技术手册等。
Videos that demonstrate each step in the western blot workflow, including SDS-PAGE, transfer, blocking, probing with antibodies, chemiluminescent detection, and stripping the blot.
A review of the enzyme-linked immunsorbant assay (ELISA), including formats (sandwich), detection methods (direct, indirect), plate coating techniques, antibodies and other probes, buffers, and kits.
Strategies for proteomic analyses, including those for relative quantitation (SILAC, ICAT, ICPL and isobaric tags) and for absolute quantitation (via SRM), and both labeled and label-free techniques.
蛋白质生物学应用说明是类似期刊形式的文章，其中讲述了使用我们丰富多样的产品所进行的研究应用及概念验证性实验.
蛋白质生物学选择指南文库提供通往不同网页的一系列链接，这些网页包含了对比信息及筛选工具，可帮助您从多种产品中选择适合研究所需的产品.
仅供科研使用，不可用于诊断目的。
