The Thermo Scientific Pierce
Renilla Luciferase Flash Assay Kit provides researchers with a highly sensitive intracellular assay for transcriptional activity of regulatory elements in mammalian whole cell lysate.
Features of the
Renilla Luciferase Flash Assay Kit:
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Sensitive—greater sensitivity allows utilization of smaller numbers of cells
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Cost effective—highest sensitivity assays result in decreased reagent consumption
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Time-saving—assays require minimal sample handling
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Automation-friendly—amenable to high throughput screens
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Convenient—contains a universal cell lysis buffer and optimized flash assay reagent
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Safe—allows one to perform non-radioactive assays
This Flash Assay Kit contains reagents for measuring the activity of
Renilla luciferase in mammalian cell lysates. When used with Thermo Scientific Green
Renilla Luc Plasmids, the kit provides an extremely sensitive bioluminescent reporter assay system for intracellular detection of promoter or pathway activity. The signal produced by Green
Renilla Luc is considerably greater than signal from either native firefly or
Renilla luciferases assayed under similar conditions. Light output captured using a luminometer can be correlated with the amount of
Renilla luciferase protein produced and used to determine the activity of the promoter driving
Renilla expression.
Includes:Cell lysis buffer, reaction buffer and substrate
Requires:Renilla luciferase and luminometer or other instrument capable of monitoring luminescence, such as Thermo Scientific Luminoskan Ascent and Varioskan Flash Microplate Readers.
Applications:• Promoter studies for analyzing
cis- regulatory elements and
trans-acting factors
• Drug screening
• siRNA and miRNA screening
• Multiplexed assays to study off-target effects
• Protein localization reporter assays
• Signal transduction pathway analysis
• RNA splicing studies
The Green
Renilla luciferase is a 36kDa protein produced by a derivative of the wild type
Renilla luciferase gene from the sea pansy,
Renilla reniformis. Compared to the wild type luciferase, Green
Renilla is more stable in serum and has an the emission spectrum that is shifted toward the green region. The protein provides extremely bright flash signal that decays rapidly. The Pierce
Renilla Flash Assay Kit reagent optimizes the this signal for use in flash-type luciferase reporter assays. The kit also is compatible for flash assays with other
Renilla luciferases and derivatives that use coelenterazine as a substrate.
More Product Data•
Luciferase assays in hard-to-transfect Jurkat cells•
Versatile luciferases: microplate luminometers and flash luciferase assaysRelated ProductsPierce™ Renilla-Firefly Luciferase Dual Assay KitFor Research Use Only. Not for use in diagnostic procedures.