Figure 1. Absorption and fluorescence emission spectra of NovaFluor Blue 610-70S dye.
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| 3 | 488 | 610/20 | 492 | 616 | (in buffer) 5 | flow cytometry |
NovaFluor Blue 610 dyes are available with two different levels of brightness: NovaFluor Blue 610-30S and NovaFluor Blue 610-70S, allowing for differential matching of the dye brightness to the antigen density or abundance, without panel redesign. NovaFluor Blue 610-70S dye is the brighter dye.
NovaFluor Blue 610-70S dye can be used to replace PE/Dazzle 594, BD Horizon PE-CF594, or PE-Texas Red dye using the 488 nm blue laser line for excitation. As compared with these PE tandem dyes, NovaFluor Blue 610-70S dye shows much less cross-excitation and lower spectral spillover with the 561 nm yellow-green laser line, allowing detection of an additional marker labeled with, for example, NovaFluor Yellow 610 dye. The macromolecule-based NovaFluor Blue 610 dyes produce highly stable fluorescence, and stained samples retain their fluorescence intensity and spectral signature when stored at 4°C. Use NovaFluor dyes with CellBlox Plus Blocking Buffer to block non-specific binding of NovaFluor labels, PE and APC tandems observed with macrophages and monocytes.
- Narrow emission spectrum and minimal cross-laser excitation for better resolution
- Higher intensity allows it to be paired with medium-to-low abundance antigens
- Can serve as a replacement for PE-tandem dyes using the blue laser, with reduced cross-laser excitation and fluorescent spillover
- Single PE-tandems can be replaced by NovaFluor Blue 610-70S and NovaFluor Yellow 610 dyes to allow detection of an additional marker in a flow cytometry panel
- NovaFluor Blue 610-30S and NovaFluor Blue 610-70S can be used together and unmixed using a spectral flow cytometer
- Produces highly stable fluorescence and is compatible with all buffers tested
Figure 2. Spectral signature of NovaFluor Blue 610-70S dye. Normal human peripheral blood cells stained with anti-CD4 antibodies (clone SK3) conjugated to NovaFluor Blue 610-70S dye were used for analysis. Data were acquired on a 5-laser Cytek Aurora system.
Figure 3. Staining of normal human peripheral blood cells with side scatter and unstained (left) or CD19 Monoclonal Antibody, NovaFluor Blue 610-70S (right). Data were acquired in the B6 channel on a 5-laser Cytek Aurora system, and singlet cells were used for analysis.
NovaFluor Blue 610-70S dye background
NovaFluor dyes, including NovaFluor Blue 610 dyes, are built using Phiton technology and are compatible with both spectral flow cytometry as well as traditional flow cytometry. NovaFluor dyes exhibit narrow emission spectra and minimal cross-laser excitation, reducing spectral spillover for better marker resolution. In addition, their unique spectral signatures can provide the opportunity to detect additional markers in flow cytometry panels by opening up previously unusable channels.
We offer NovaFluor dyes conjugated to primary antibodies for use in flow cytometry, as well as NovaFluor Antibody Conjugation Kits, NovaFluor CD4 Label Characterization Kits, and custom conjugation services.
Additional resources
Flow cytometry protocols handbook
Protocols that fit your needs in flow cytometry ranging from sample preparation to numerous cell stimulation conditions, staining, immunophenotyping, and data analysis strategies.
Fluorophore and reagent selection guide for flow cytometry
A handy reference poster featuring the broad range of our available dyes and labeling reagents.
Imaging protocol handbook
Protocols that fit your needs in imaging ranging from sample and assay preparation to staining, labeling, and data analysis strategies.
Fluorophore and reagent selection guide for cell imaging
A tool for selecting the optimal fluorescent dyes for your Invitrogen EVOS cell imaging systems.
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