Flow cytometry protocols handbook
Protocols that fit your needs in flow cytometry ranging from sample preparation to numerous cell stimulation conditions, staining, immunophenotyping, and data analysis strategies.
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| 3 | 532 | 555/20 | 534 | 553 | (in buffer): 2 (in antifade): 3 | microscopy, flow cytometry, fluorescence microplate reader |
Alexa Fluor 532 is a yellow-fluorescent dye with excitation designed for use with the frequency-doubled Nd:YAG laser line. Alexa Fluor 532 dye is pH-insensitive over a wide molar range, helping ensure that you get stable signals in live-cell imaging applications.
Alexa Fluor 532 conjugates exhibit an excitation maximum at 532 nm and emission maximum at 554 nm. It is particularly useful in multicolor applications, including super-resolution microscopy using dSTORM.
We offer Alexa Fluor 532 dye conjugated to a variety of antibodies and peptides optimized for cellular labeling and detection. In addition, reactive dye forms and protein labeling kits are available to enable you to generate your own antibody conjugates or probes.
Protein Labeling Reagents Selection Guide (NHS ester, maleimide, etc.) Search Alexa Fluor 532 primary antibodies
Figure 1. Human mononuclear cells were stained with mouse anti human CD4 biotin, washed, stained with streptavidin, Alexa Fluor 532 conjugate, washed, and analyzed by flow cytometry using 532 nm excitation.
Figure 2. A bovine pulmonary artery endothelial cell labeled with mouse monoclonal anti-a-tubulin antibody in combination with Alexa Fluor 532 goat anti-mouse IgG antibody to stain microtubules. The image was acquired using a bandpass filter set (excitation/emission 535 +- 17.5/590 +- 17.5 nm).
Additional resources
Fluorophore and reagent selection guide for flow cytometry
A handy reference poster featuring the broad range of our available dyes and labeling reagents.
Imaging protocol handbook
Protocols that fit your needs in imaging ranging from sample and assay preparation to staining, labeling, and data analysis strategies.
Fluorophore and reagent selection guide for cell imaging
A tool for selecting the optimal fluorescent dyes for your Invitrogen EVOS cell imaging systems.
Cy™ is a trademark or registered trademark of GE Healthcare.
Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Brilliant Ultra Violet™ and Brilliant Violet™ are trademarks or registered trademarks of Becton, Dickinson and Company or its affiliates, and are used under license.
For Research Use Only. Not for use in diagnostic procedures.