Comparison of fluorescence intensity of eFluor 506 dye with Horizon V500. Mouse splenocytes were stained with CD4 Monoclonal Antibody (RM4-5), eFluor 506 (purple line) or Horizon V500 (blue histogram), using the same concentration of antibody.
 |  |  |  |  |  |  |
| 1 | 405 | 510/50 | 419 | 508 | (in buffer) 1 | flow cytometry |
The eFluor 506 organic dye is a violet-laser excitable fluorophore with an emission peak of 506 nm and is optimized for use in flow cytometry applications. With similar spectra characteristics to AmCyan and Horizon V500 dyes, conjugates of eFluor 506 can be easily added to your multicolor panels without change in instrument configuration for use.
We offer eFluor 506 dye conjugated to primary antibodies for use in flow cytometry.
Additional products
- Direct alternative for AmCyan and Horizon V500 fluorophores
- Similar or better fluorescence intensity than other 500 nm-emitting organic dyes
- When combined with all five Super Bright dye antibody conjugates, can provide 6 detection channels of the violet laser
Stability of eFluor 506 dye. Mouse splenocytes were stained with CD4 Monoclonal Antibody (RM4-5), eFluor 506 and either left unfixed (blue line), or were fixed for 30 minutes in Foxp3/Transcription Factor Buffer Set (purple line) or 30 minutes in IC Fixation Buffer followed by methanol (orange line).
Additional resources
Flow cytometry protocols handbook
Protocols that fit your needs in flow cytometry ranging from sample preparation to numerous cell stimulation conditions, staining, immunophenotyping, and data analysis strategies.
Fluorophore and reagent selection guide for flow cytometry
A handy reference poster featuring the broad range of our available dyes and labeling reagents.
Imaging protocol handbook
Protocols that fit your needs in imaging ranging from sample and assay preparation to staining, labeling, and data analysis strategies.
Fluorophore and reagent selection guide for cell imaging
A tool for selecting the optimal fluorescent dyes for your Invitrogen EVOS cell imaging systems.
Cy™ is a trademark or registered trademark of GE Healthcare.
Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Brilliant Ultra Violet™ and Brilliant Violet™ are trademarks or registered trademarks of Becton, Dickinson and Company or its affiliates, and are used under license.
For Research Use Only. Not for use in diagnostic procedures.