Flow cytometry protocols handbook
Protocols that fit your needs in flow cytometry ranging from sample preparation to numerous cell stimulation conditions, staining, immunophenotyping, and data analysis strategies.
 |  |  |  |  |  |  |
| 4 | 488 | 530/30 | 490 | 525 | (in buffer) 2 (in antifade) 4 | microscopy, flow cytometry, fluorescence microplate reader |
Invitrogen Alexa Fluor 488 dye is a bright, green-fluorescent dye with excitation designed for use with the 488 nm laser line. For stable signal generation in imaging and flow cytometry, Alexa Fluor 488 dye is pH-insensitive over a wide molar range. We offer Alexa Fluor 488 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates, optimized for cellular labeling and detection. In addition, reactive dye forms and protein labeling kits are available to allow you to generate your own antibody conjugates or probes.
Protein Labeling Reagents Selection Guide (NHS ester, maleimide, etc.) Search Alexa Fluor 488 secondary antibodies
Probes with high fluorescence quantum yield and high photostability allow detection of low-abundance biological structures with greater sensitivity. Alexa Fluor 488 dye molecules can be attached to proteins at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection.
The actin cytoskeleton of this muntjack fibroblast was visualized using the green-fluorescent Alexa Fluor 488 Phalloidin, and the nucleus was stained with TO-PRO-3 reagent.
C57BL/6 mouse splenocytes were stained intracellularly, using the Foxp3/Transcription Factor Staining Buffer Set and protocol, with CD4 Monoclonal Antibody, APC and 0.125 µg of Mouse IgG2a kappa Isotype Control, Alexa Fluor 488 (left) or 0.125 µg of FOXP3 Monoclonal Antibody, Alexa Fluor 488 (right). Cells in the lymphocyte gate were used for analysis.
Additional resources
Fluorophore and reagent selection guide for flow cytometry
A handy reference poster featuring the broad range of our available dyes and labeling reagents.
Imaging protocol handbook
Protocols that fit your needs in imaging ranging from sample and assay preparation to staining, labeling, and data analysis strategies.
Fluorophore and reagent selection guide for cell imaging
A tool for selecting the optimal fluorescent dyes for your Invitrogen EVOS cell imaging systems.
Cy™ is a trademark or registered trademark of GE Healthcare.
Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Brilliant Ultra Violet™ and Brilliant Violet™ are trademarks or registered trademarks of Becton, Dickinson and Company or its affiliates, and are used under license.
For Research Use Only. Not for use in diagnostic procedures.