Qdot 565 probe

Qdot 565 probe is an excellent orange-fluorescent label with outstanding brightness and photostability for imaging and flow cytometry applications. With an emission maximum of ~565 nm, Qdot 565 probe can be excited using a 350, 488, or 525 nm laser line or standard TRITC filter. Optimum imaging results with brighter signals are achieved with a Qdot 565 filter or light cube.

Made from nanocrystals of semiconductor material, Qdot probes are broadly compatible with standard fluorescence techniques, but there are some novel aspects to their use that can be optimized to obtain the best possible results. In general they have an extremely broad excitation range and narrow emission with a large Stokes shift. Optimal brightness is generally achieved using a specific Qdot filter.

Organic fluorophores and Qdot probes can be readily combined in multiplexed flow cytometry or imaging studies. Longer-wavelength Qdot probes are also ideal for multiplexed western blots.

Qdot 565 probe dashboard


Initial brightness
 
 
 
 
 

For best results use a Qdot-optimized filter and PBS or a mounting medium without antifade.

 

 

Photostability in buffer

 
 
 
 
 
 350, 488, 525
TRITCUV565 
 
Laser line
Common filter set
Excitation max
Emission max		 

Qdot 565 products

The exceptional brightness and photostability of Qdot probes make it easier to detect low-abundance targets. With narrow and symmetric emission spectra, Qdot probes are also ideal for multiplexed fluorescence detection using a combination of Qdot probes and organic dyes.

qdot565-figure1

Qdot 565 conjugation kits

We’ve developed functionalized versions of the Qdot 565 label for the preparation of custom conjugates of proteins and other biomolecules. The first—a carboxyl-derivatized form—can be coupled to amine groups of proteins and modified oligonucleotides. The second—an amine-derivatized PEG form—can be coupled to isothiocyanates and succinimidyl esters (or with native carboxylic acids using water-soluble carbodiimides).

AmineAldehyde-, ketone, carboxylate or phosphate
Qdot probes and accessories

Qdot probes and accessories

Qdot Probes are made from nanocrystals of semiconductor material and are broadly compatible with standard fluorescence techniques, but there are some novel aspects to their use that can be optimized to obtain the best possible results.