Flow cytometry protocols handbook
Protocols that fit your needs in flow cytometry ranging from sample preparation to numerous cell stimulation conditions, staining, immunophenotyping, and data analysis strategies.
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Invitrogen BODIPY FL dye is a bright, green-fluorescent dye with excitation and emission similar to fluorescein (FITC) or Alexa Fluor 488 dye. It has a high extinction coefficient and fluorescence quantum yield and is relatively insensitive to solvent polarity and pH change. In contrast to the highly water-soluble fluorophores Alexa Fluor 488 dye and fluorescein (FITC), BODIPY dyes have unique hydrophobic properties excellent for staining lipids, membranes, and other lipophilic compounds.
BODIPY FL dye has a relatively long excited-state lifetime (typically 5 nanoseconds or longer), which is useful for fluorescence polarization–based assays and a large two-photon cross-section for multiphoton excitation.
We offer BODIPY FL dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling and detection. In addition, reactive dye forms and protein labeling kits are available to help you generate your own protein conjugates or probes.
Additional products
Fluorescence microscopic analysis of cellular components in BPAEC.FluoCells Prepared Slide #2, shows bovine pulmonary artery endothelial cells (BPAEC) stained with alpha-Tubulin Monoclonal Antibody in conjunction with Goat anti-Mouse IgG Cross-Adsorbed Secondary Antibody, BODIPY FL for labeling microtubules. Texas Red-X Phalloidin was used for labeling F-actin and DAPI for labeling the nuclei. This multiple-exposure image was acquired using bandpass optical filter sets appropriate for DAPI, FITC and Texas Red dye.
Additional resources
Fluorophore and reagent selection guide for flow cytometry
A handy reference poster featuring the broad range of our available dyes and labeling reagents.
Imaging protocol handbook
Protocols that fit your needs in imaging ranging from sample and assay preparation to staining, labeling, and data analysis strategies.
Fluorophore and reagent selection guide for cell imaging
A tool for selecting the optimal fluorescent dyes for your Invitrogen EVOS cell imaging systems.
Cy™ is a trademark or registered trademark of GE Healthcare.
Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Brilliant Ultra Violet™ and Brilliant Violet™ are trademarks or registered trademarks of Becton, Dickinson and Company or its affiliates, and are used under license.
For Research Use Only. Not for use in diagnostic procedures.