Flow cytometry protocols handbook
Protocols that fit your needs in flow cytometry ranging from sample preparation to numerous cell stimulation conditions, staining, immunophenotyping, and data analysis strategies.
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| 4 | 488 | FITC | 488 | 510 | (in buffer) 2 (in antifade) 2 | microscopy, flow cytometry |
Invitrogen Green Fluorescent Protein (GFP) is a useful expression label in the blue channel for flow cytometry and imaging applications. GFP can be excited by the 488 nm laser line and is optimally detected at 510 nm. It is a versatile biological marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression in vivo.
We offer a series of GFP Invitrogen CellLight fusion constructs of signal peptides or cell structure proteins with emGFP for accurate and specific targeting to subcellular structures, including the cytoskeleton, mitochondria, and secretory compartments.
To complement these tools, we also offer anti-GFP antibodies and antibody conjugates for a wide variety of applications, including imaging, western blotting, and flow cytometry. Our anti-GFP antibodies are suited for detection of native GFP, GFP variants, and CellLight fusion proteins.
Dual labeling of NIH 3T3 cells with GFP and anti-GFP antibody. NIH 3T3 cells were transiently transfected with a Green Fluorescent Protein (GFP) expression vector, plated and allowed to attach and proliferate. The cells were fixed and labeled with our GFP Polyclonal Antibody, Alexa Fluor 594. Cells expressing GFP showed dual labeling of both GFP (green fluorescence) and the anti-GFP antibody (red fluorescence). In this overlay of fluorescence and differential interference contrast (DIC) micrographs, the GFP-transfected cells exhibit green and red signals that overlap to yield yellow, and DAPI stains the nuclei with a light-blue fluorescence. In the untransfected cells, the DAPI-stained nuclei exhibited a bright-blue fluorescence.
Live-cell imaging of HeLa cells with CellLight reagents using BacMam technology. HeLa cells were transduced with CellLight Mitochondria-GFP, BacMam 2.0 and CellLight Talin-RFP. The following day, cells were stained with Hoechst 33342 and live-cell imaging was performed using DeltaVision Core microscope and standard DAPI/FITC/TRITC filter sets.
CellLight reagents
CellLight reagents are ready-to-use fluorescent protein constructs targeted to specific subcellular structures. These reagents provide a simple and effective method for introducing targeted intracellular labels within living cells. Simply add the reagent to your cells, incubate overnight, and you’re ready to image your cells.
Additional resources
Fluorophore and reagent selection guide for flow cytometry
A handy reference poster featuring the broad range of our available dyes and labeling reagents.
Imaging protocol handbook
Protocols that fit your needs in imaging ranging from sample and assay preparation to staining, labeling, and data analysis strategies.
Fluorophore and reagent selection guide for cell imaging
A tool for selecting the optimal fluorescent dyes for your Invitrogen EVOS cell imaging systems.
Cy™ is a trademark or registered trademark of GE Healthcare.
Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Brilliant Ultra Violet™ and Brilliant Violet™ are trademarks or registered trademarks of Becton, Dickinson and Company or its affiliates, and are used under license.
For Research Use Only. Not for use in diagnostic procedures.
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