Table 23.1 Spectral characteristics of Molecular Probes dyes.
| Dye | Excitation Maximum (nm)* | Emission Maximum (nm)* |
|---|---|---|
| Acridine orange | 500 (+DNA) 460 (+RNA) | 526 (+DNA) 650 (+RNA) |
| Alexa Fluor 350 dye, 7-amino-4-methylcoumarin (AMC) | 346 | 442 |
| Alexa Fluor 405 dye | 402 | 421 |
| Alexa Fluor 430 dye | 434 | 539 |
| Alexa Fluor 488 dye | 495 | 519 |
| Alexa Fluor 514 dye | 518 | 540 |
| Alexa Fluor 532 dye | 531 | 554 |
| Alexa Fluor 546 dye | 556 | 573 |
| Alexa Fluor 555 dye | 555 | 565 |
| Alexa Fluor 568 dye | 578 | 603 |
| Alexa Fluor 594 dye | 590 | 617 |
| Alexa Fluor 610 dye | 612 | 628 |
| Alexa Fluor 633 dye | 632 | 647 |
| Alexa Fluor 635 dye | 633 | 647 |
| Alexa Fluor 647 dye | 650 | 668 |
| Alexa Fluor 660 dye | 663 | 690 |
| Alexa Fluor 680 dye | 679 | 702 |
| Alexa Fluor 700 dye | 702 | 723 |
| Alexa Fluor 750 dye | 749 | 775 |
| Alexa Fluor 790 dye | 782 | 805 |
| Allophycocyanin | 650 | 660 |
| 7-Aminoactinomycin D (7-AAD) † | 546 | 647 |
| BCECF indicator | 482 (low pH) 503 (high pH) | 520 (low pH) 528 (high pH) |
| Bimane | 380 | 458 |
| BOBO-1, BO-PRO-1 | 462 | 481 |
| BODIPY 630/650 dye | 630 | 650 |
| BODIPY 650/665 dye | 650 | 665 |
| BODIPY FL dye | 505 | 513 |
| BODIPY TMR-X dye | 542 | 574 |
| BODIPY TR-X dye | 589 | 617 |
| Calcein | 494 | 517 |
| Calcium Crimson indicator | 590 | 615 |
| Calcium Green indicators | 506 | 533 |
| Calcium Orange indicator | 549 | 576 |
| Carboxy SNARF indicators | 548 (low pH) 576 (high pH) | 587 (low pH) 635 (high pH) |
| 6-Carboxyrhodamine 6G | 525 | 555 |
| Cascade Blue dye | 400 | 420 |
| Cascade Yellow dye | 402 | 545 |
| DAPI † | 358 | 461 |
| Di-8-ANEPPS, Di-4-ANEPPS ‡ | 468 | 635 |
| DiA ‡ | 456 | 590 |
| DiD (DiIC18(5)) | 644 | 665 |
| DiI (DiIC18(3)) | 549 | 565 |
| DiO (DiOC18(3)) | 484 | 501 |
| DiR (DiIC18(7)) | 750 | 779 |
| ELF 97 alcohol | 345 | 530 |
| Eosin | 524 | 544 |
| ER Tracker Blue-White DPX ‡ | 372 | 556 |
| EthD-1 † | 528 | 617 |
| Ethidium bromide † | 518 | 605 |
| Fluo-3 indicator | 506 | 526 |
| Fluo-4 indicator | 494 | 516 |
| Fluorescein, FITC | 494 | 518 |
| FM 1-43, FM 1-43FX ‡ | 479 | 598 |
| FM 4-64, FM 4-64FX ‡ | 506 | 750 |
| Fura-2 indicator | 363 (low [Ca2+]) 335 (high [Ca2+]) | 512 (low [Ca2+]) 505 (high [Ca2+]) |
| Fura Red indicator | 472 (low [Ca2+]) 436 (high [Ca2+]) | 657 (low [Ca2+]) 637 (high [Ca2+]) |
| Hoechst 33258, Hoechst 33342 † | 352 | 461 |
| 7-Hydroxy-4-methylcoumarin | 360 | 455 |
| Indo-1 indicator | 346 (low [Ca2+]) 330 (high [Ca2+]) | 475 (low [Ca2+]) 401 (high [Ca2+]) |
| JC-1 | 498 (monomer) 593 (aggregate) | 525 (monomer) 595 (aggregate) |
| JC-9 | 506 | 525 (monomer) 635 (aggregate) |
| JOE dye | 525 | 555 |
| Lissamine rhodamine B | 570 | 590 |
| Lucifer yellow CH | 428 | 536 |
| LysoSensor Blue DND-167 | 374 | 425 |
| LysoSensor Green DND-153, DND-189 | 442 | 505 |
| LysoSensor Yellow/Blue DND-160 (PDMPO) | 384 (low pH) 329 (high pH) | 540 (low pH) 440 (high pH) |
| LysoTracker Green | 504 | 511 |
| LysoTracker Red | 577 | 592 |
| Magnesium Green indicator | 506 | 531 |
| Marina Blue dye | 365 | 460 |
| MitoTracker Green FM | 490 | 516 |
| MitoTracker Orange CMTMRos | 551 | 576 |
| MitoTracker Red CMXRos | 578 | 599 |
| Monobromobimane § | 394 | 490 |
| NBD amines | 465 | 535 |
| NeuroTrace 500/525 green-fluorescent Nissl stain † | 500 | 525 |
| Nile red ‡ | 549 | 628 |
| Oregon Green 488 dye and Oregon Green 488 BAPTA indicators | 496 | 524 |
| Oregon Green 514 dye | 511 | 530 |
| Pacific Blue dye | 410 | 455 |
| Pacific Orange dye | 400 | 551 |
| POPO-1, PO-PRO-1 † | 434 | 456 |
| Propidium iodide † | 536 | 617 |
| Rhodamine 110 | 496 | 520 |
| Rhodamine Red dye | 570 | 590 |
| R-phycoerythrin | 565 | 575 |
| Resorufin | 571 | 585 |
| RH 414 ‡ | 500 | 635 |
| Rhod-2 indicator | 552 | 578 |
| Rhodamine Green dye | 502 | 527 |
| Rhodamine 123 | 507 | 529 |
| ROX dye | 580 | 605 |
| Sodium Green indicator | 507 | 535 |
| SYTO blue-fluorescent nucleic acid stains 40, 41, 42, 43 † | 428 ± 6 | 454 ± 13 |
| SYTO blue-fluorescent nucleic acid stains 44, 45 † | 450 ± 5 | 478 ± 7 |
| SYTO green-fluorescent nucleic acid stains 12, 13, 16, 21, 23, 24 † | 494 ± 6 | 515 ± 7 |
| SYTO green-fluorescent nucleic acid stains 11, 14, 15, 20, 22, 25 † | 515 ± 7 | 543 ± 13 |
| SYTO orange-fluorescent nucleic acid stains 80, 81, 82, 83 † | 537 ± 7 | 552 ± 8 |
| SYTO orange-fluorescent nucleic acid stains 84, 85 † | 567 | 583 |
| SYTO red-fluorescent nucleic acid stains 17, 59, 61, 64 † | 615 ± 15 | 632 ± 13 |
| SYTO red-fluorescent nucleic acid stains 60, 62, 63 † | 655 ± 3 | 675 ± 3 |
| SYTOX Blue nucleic acid stain † | 445 | 470 |
| SYTOX Green nucleic acid stain † | 504 | 523 |
| SYTOX Orange nucleic acid stain † | 547 | 570 |
| Tetramethylrhodamine, Rhodamine B | 555 | 580 |
| Texas Red and Texas Red-X dye | 595 | 615 |
| TOTO-1, TO-PRO-1 † | 514 | 533 |
| TOTO-3, TO-PRO-3 † | 642 | 660 |
| X-rhod-1 indicator | 580 | 602 |
| YOYO-1, YO-PRO-1 † | 491 | 509 |
| YOYO-3, YO-PRO-3 † | 612 | 631 |
| * Fluorescence excitation and emission maxima in nm are listed here, and fluorescence spectra are available at (thermofisher.com/spectraviewer). Transmittance curves and other specific microscopy filter information can be found through microscope manufacturers or at filter manufacturer websites, including Chroma Technology Corp. (www.chroma.com), Omega Optical, Inc. (www.omegafilters.com) and Semrock, Inc. (www.semrock.com). † Excitation and emission peaks listed for dye/nucleic acid complex. ‡ Excitation and emission peaks listed for lipid-bound dye. § Excitation and emission peaks listed for glutathione-conjugated dye. | ||
For Research Use Only. Not for use in diagnostic procedures.