Table 19.3 Parallel performance comparison of fluo-3 and fluo-4 on Molecular Devices FLIPR system.
| Indicator | µM * | Time *(min) |
Fluorescence †
| Increase § | |
|---|---|---|---|---|---|
| Basal | Stimulated ‡ | ||||
| Fluo-3 | 4 | 60 | 1700 | 5700 | 3.4-fold |
| Fluo-4 | 4 | 60 | 4900 | 21300 | 4.3-fold |
| Fluo-4 | 2 | 30 | 1200 | 5400 | 4.5-fold |
| * CHO cells stably transfected with rat muscarinic M1 receptors were loaded with fluo-3 AM or fluo-4 AM according to standard protocols, with variations in indicator concentration (µM) and incubation time, as shown. † Relative fluorescence intensities after subtraction of background fluorescence. ‡ Cells were stimulated by addition of the muscarinic agonist carbachol (50 µM). § Ratio of stimulated to basal fluorescence intensities. | |||||
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