Table 19.6 Fluorescent indicators for Zn2+.
| Indicator | Water-Soluble Salt | Cell-Permeant Ester | Ex/Em * | Kd for Zn2+ † |
|---|---|---|---|---|
| FluoZin-1 | F24180 | F24181 | 495/515 | 8 µM |
| FluoZin-2 | NA | F24189 | 495/525 | 2 µM |
| FluoZin-3 | F24194 | F24195 | 494/516 | 15 nM ** |
| RhodZin-3 | R36350 | R36351 | 550/575 | 65 nM |
| Newport Green DCF | N7990 | N7991 | 505/535 | 1 µM |
| Newport Green PDX | NA | N24191 | 495/520 | 30 µM |
| * Excitation (Ex) and Emission (Em) maxima, in nm. † Dissociation constant of the indicator Zn2+ complex measured in 50 mM MOPS, pH 7.0, at 22°C. ‡ Excitation peak shifts upon binding Zn2+. § Emission peak shifts upon binding Zn2+. ** Kd determined in 135 mM NaCl, 1.1 mM EGTA, 20 mM HEPES, pH 7.4, 0–10 µM free Zn2+ at 22°C. NA = Not available. | ||||
For Research Use Only. Not for use in diagnostic procedures.